Sulfo-NHS-SS-Biotin sodium

Name: Sulfo-NHS-SS-Biotin sodium
Brand: MedChemExpress
SKU: HY-111496
Rating: 5.0 (4 reviews)

製品番号: HY-111496 純度: 97.0%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Sulfo-NHS-SS-biotin sodium is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin sodium can be used for the labeling and purifying of cell-surface protein.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 325143-98-4

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 113	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 113	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$85	在庫あり	Estimated Time of Arrival: December 31
10 mg	$140	在庫あり	Estimated Time of Arrival: December 31
25 mg	$238	在庫あり	Estimated Time of Arrival: December 31
50 mg	$310	在庫あり	Estimated Time of Arrival: December 31
100 mg	$400	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 4 publication(s) in Google Scholar

顧客検証

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 May 31:1-21. [Abstract]; 293T cells were treated with 1 mL PBS-CM supplemented with Sulfo-NHS-SS-Biotin sodium (0.5 mg/mL; 30 min; 40 °C) with gentle agitation. Immunoblot analysis of cell surface-biotinylated ZDHHC5 was performed.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Sep 29;26(19):9522.; HT-22 cells were incubated in Hank's balanced salt solution containing Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 30 min; 40 ℃) to biotinylate membrane proteins. Expression level of biotinylated GFRα1 following 24 h exposure to 2 mM METH was measured.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Jan 10:154047. [Abstract]; Total TrkB, pTrkB (Tyr516), membrane TrkB, biotinylated TrkB, and HAP1 expression levels in HT-22 cells after incubation with METH (2 mM) for 24 h. HT-22 cells were first biotinylated in Hanks’ balanced salt solution (HBSS) containing 1 mg/mL Sulfo-NHS-SS-biotin sodium for 30 min at 4 °C, then rinsed three times with HBSS.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Jan 10:154047. [Abstract]; Levels of total TrkB, pTrkB (Tyr516), biotinylated TrkB, BDNF, and apoptosis-related proteins in hippocampal slices after incubation with METH (2 mM) for 24 h. Hippocampal slices were first biotinylated in Hanks’ balanced salt solution (HBSS) containing 1 mg/mL Sulfo-NHS-SS-biotin sodium for 30 min at 4 °C, then rinsed three times with HBSS.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11. [Abstract]; BV2 cells were collected and were labeled with Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 1 h; 4 ℃;). Membrane proteins extracted were analyzed by Western blotting.

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製品説明

IC₅₀ & Target

IC50: cell-surface protein^[1]

体外実験

Sulfo-NHS-SS-Biotin sodium is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes^[1].
Sulfo-NHS-SS-Biotin sodium reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides^[1].
Sulfo-NHS-SS-Biotin sodium has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns^[1].
Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS).
The biotinylation reactions are terminated with 100 mM glycine in PBS.
After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010).
Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

606.69

分子式

C₁₉H₂₇N₄NaO₉S₄

CAS 番号

325143-98-4

Appearance

Solid

Color

White to yellow

SMILES

O=C(ON1C(C(S(=O)(O[Na])=O)CC1=O)=O)CCSSCCNC(CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O)=O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (164.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6483 mL	8.2414 mL	16.4829 mL
5 mM	0.3297 mL	1.6483 mL	3.2966 mL
10 mM	0.1648 mL	0.8241 mL	1.6483 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 97.0%

Select Batch:

データシート (271 KB) SDS (252 KB)

COA (245 KB) HNMR (300 KB) MS (231 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Chia CP,et al. Phagocytosis in Dictyostelium discoideum is inhibited by antibodies directed primarily against common carbohydrate epitopes of a major cell-surface plasma membrane glycoprotein.Exp Cell Res. 1989 Mar;181(1):11-26. [Content Brief]

[2]. Jo M, et al. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 1;70(21):8948-58. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6483 mL	8.2414 mL	16.4829 mL	41.2072 mL
	5 mM	0.3297 mL	1.6483 mL	3.2966 mL	8.2414 mL
	10 mM	0.1648 mL	0.8241 mL	1.6483 mL	4.1207 mL
	15 mM	0.1099 mL	0.5494 mL	1.0989 mL	2.7471 mL
	20 mM	0.0824 mL	0.4121 mL	0.8241 mL	2.0604 mL
	25 mM	0.0659 mL	0.3297 mL	0.6593 mL	1.6483 mL
	30 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3736 mL
	40 mM	0.0412 mL	0.2060 mL	0.4121 mL	1.0302 mL
	50 mM	0.0330 mL	0.1648 mL	0.3297 mL	0.8241 mL
	60 mM	0.0275 mL	0.1374 mL	0.2747 mL	0.6868 mL
	80 mM	0.0206 mL	0.1030 mL	0.2060 mL	0.5151 mL
	100 mM	0.0165 mL	0.0824 mL	0.1648 mL	0.4121 mL