SYBR Green II 

SYBR Green II is a fluorescent nucleic acid dye that mainly binds single-stranded nucleotides. SYBR Green II is sensitive to oligonucleotides or larger nucleic acid polymers in a variety of cells and gels. SYBR Green II can be used to study cell structure, membrane integrity or function, and cell cycle distribution. Wavelength 484/515 nm^[1].

SYBR Green II binds to RNA or single-stranded DNA, and emits fluorescence. SYBR Green II can be used for RNA/DNA staining before or after electrophoresis^[2].
This product is supplied as a concentrate dissolved in DMSO. It is recommended to dilute it 10,000-fold before use.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

581.51

C₂₈H₂₈IN₃OS

195199-08-7

Liquid

Light yellow to orange

CN1/C(OC2=CC=CC=C12)=C/C3=C4C=CC=CC4=[N+](C5=CC=CC=C5)C(SCCN(C)C)=C3.[I-]

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

• [1]. Yue S T, et al. Substituted unsymmetrical cyanine dyes with selected permeability: U.S. Patent 5,658,751[P]. 1997-8-19.

  [2]. Berdalet E, et al., Quantifying RNA and DNA in planktonic organisms with SYBR Green II and nucleases. Part B. Quantification in natural samples[J]. 2005.