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TNS sodium  (Synonyms: 6-(p-Tolylamino)naphthalene-2-sulfonate sodium)

Art. -Nr.: HY-W250727 Reinheit: 99.97%
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SDS
Handling Instructions Technical Support

TNS (6-(p-Tolylamino) naphthalene-2-sulfonate) sodium is a fluorescent probe used to detect the conformational state of proteins. TNS sodium binds to the central hydrophobic calyx of β-lactoglobulin (β-LG) to form a 1:1 complex, which stabilizes the folded conformation of β-LG. TNS sodium shows weak fluorescent signals in aqueous solution, but its fluorescence intensity increases significantly upon binding to the hydrophobic domains of proteins, with the maximum excitation wavelength and maximum emission wavelength located at 321 nm and 446 nm, respectively.

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TNS sodium

TNS sodium Chemische Struktur

CAS. Nr. : 53313-85-2

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Based on 1 publication(s) in Google Scholar

TNS sodium Related Antibodies

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1 Publications Citing Use of MCE TNS sodium

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Beschreibung

TNS (6-(p-Tolylamino) naphthalene-2-sulfonate) sodium is a fluorescent probe used to detect the conformational state of proteins. TNS sodium binds to the central hydrophobic calyx of β-lactoglobulin (β-LG) to form a 1:1 complex, which stabilizes the folded conformation of β-LG. TNS sodium shows weak fluorescent signals in aqueous solution, but its fluorescence intensity increases significantly upon binding to the hydrophobic domains of proteins, with the maximum excitation wavelength and maximum emission wavelength located at 321 nm and 446 nm, respectively[1].

In Vitro

TNS (10 μM TNS, 10 μM β-LG; 298 K) sodium binds to purified bovine β-lactoglobulin (β-LG) at a distance of 2.5 nm from the Trp19 residue of β-LG at pH 7.0[1].
TNS (sodium) (25 μM TNS, 25 μM β-LG; incubated with 0-1.2 M NaCl) binding to purified bovine β-lactoglobulin (β-LG) at pH 7.0 is disrupted by increasing ionic strength (via NaCl), with the most significant disruption occurring at NaCl concentrations between 0 M and 0.25 M[1].
TNS (10 μM TNS, 10 μM β-LG; 20 min) sodium binding to purified bovine β-lactoglobulin (β-LG) at pH 7.0 stabilizes its fold, increasing the guanidine hydrochloride concentration required for unfolding from a midpoint of 2.3 M to 3.9 M[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TNS sodium Related Antibodies
Molekulargewicht

335.36

Formel

C17H14NNaO3S
CAS. Nr.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C2C=C(NC3=CC=C(C)C=C3)C=CC2=C1)(O[Na])=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 4 mg/mL (11.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9819 mL 14.9094 mL 29.8187 mL
5 mM 0.5964 mL 2.9819 mL 5.9637 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation

Purity: 99.97%

SDS (393 KB)

COA (247 KB) HNMR (260 KB) RP-HPLC (246 KB) MS (68 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9819 mL 14.9094 mL 29.8187 mL 74.5468 mL
5 mM 0.5964 mL 2.9819 mL 5.9637 mL 14.9094 mL
10 mM 0.2982 mL 1.4909 mL 2.9819 mL 7.4547 mL
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TNS sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TNS53313-85-26-(p-Tolylamino)naphthalene-2-sulfonateFluorescent Dyesodium dodecylbenzene sulfonateguanidine hydrochlorideTrp19 residueelectrostatic interactionscentral hydrophobic calyxbovine β-LGβ-lactoglobulinsodium dodecyl sulfatehydrophobic interactionshydrogen bondsInhibitorinhibitorinhibit

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