Tyrosinase, Mushroom  (Synonyms: Polyphenol oxidase)

Tyrosinase (EC 1.14.18.1) (Polyphenol oxidase) is a rate-limiting enzyme that controls the production of melanin. Tyrosinase is mainly found in melanosomes synthesized by skin melanocytes. Tyrosinase can be used in research on Parkinson's disease, melanoma and pigmentation^[1][2][3].

Product Information
Optimal pH: 6.0-7.0
Theoretical molecular weight: 128 kDa (tetramer)

Tyrosinase activity assay
Enzyme solution preparation
Dissolve in ddH₂O at a concentration of 1 mg/mL. Further dilute to a concentration of 200-400 U/mL.
Experimental method
Adjust the spectrophotometer to 280 nm and 25°C. Add the sample to each colorimetric tube in the following order:
1mL 0.5 M Phosphate buffer, pH 6.5
1 mL 0.001 M L-Tyrosine
0.9 mL ddH₂O
Oxygen is introduced into the colorimetric tube through a capillary for 4-5 min to oxidize the reaction mixture. Place the colorimetric tube on the spectrophotometer and record the A280 at 4-5 min as the absorbance of the blank group. Add 0.1 mL of appropriately diluted enzyme and record the A280 at 10-12 min.
Calculation
1U = the amount of enzyme that causes the absorbance at 280 nm to increase by 0.001 per minute.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9002-10-2

Solid

Light brown to brown

[Tyrosinase, Mushroom]

1.14.18.1

≥500 U/mg soild

One unit is defined as the amount of enzyme that causes an increase in the absorbance at 280 nm of 0.001 in per minute at 25°C, pH 6.5, using L-tyrosine as substrate.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sánchez-Ferrer A, et al. Tyrosinase: a comprehensive review of its mechanism. Biochim Biophys Acta. 1995 Feb 22;1247(1):1-11.  [Content Brief]

  [2]. Peng LH, et al. Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin. Phytomedicine. 2013 Sep 15;20(12):1082-7.  [Content Brief]

  [3]. Nagatsu T, et al. Neuromelanin in Parkinson's Disease: Tyrosine Hydroxylase and Tyrosinase. Int J Mol Sci. 2022 Apr 10;23(8):4176.  [Content Brief]