Vadadustat prodrug-1

Vadadustat prodrug-1 (Compound 11) (100 μM; 1-10 min irradiation) enables complete and rapid near-infrared-induced photorelease of Vadadustat (HY-101277) within 10 min under physiological conditions, and exhibits excellent stability under white light, reductive environments and physiological temperatures^[1].
Vadadustat prodrug-1 (10 min irradiation prior to 1 h incubation) potently inhibits PHD2 only after 10 min of irradiation at 650 nm, with an IC₅₀ of 71.6 nM, and shows no activity under dark conditions^[1].
Vadadustat prodrug-1 (5-10 μM; 24 h reoxygenation, 10 min irradiation where applicable) exerts dose-dependent neuroprotective effects on HT22 cells treated with oxygen-glucose deprivation/reoxygenation (OGD/R). It enhances cell viability, increases the number of viable cells and reduces the number of dead cells only after 10 min of 650 nm irradiation, with no effect under dark conditions^[1].
Vadadustat prodrug-1 (5-10 μM; 10 min irradiation where applicable) dose-dependently stabilizes HIF-1α in HT22 cells, reduces OGD/R-induced ROS accumulation, restores SOD activity and mitochondrial membrane potential, and decreases MDA levels ^[1], but only after 10 min of irradiation with 650 nm light.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.