α-Cyano-4-hydroxycinnamic acid (Synonyms: α-Cyano-4-hydroxycinnamate)

Name: α-Cyano-4-hydroxycinnamic acid
Brand: MedChemExpress
SKU: HY-107641
Rating: 5.0 (11 reviews)

Cat. No.: HY-107641 Purity: 99.92%: Data Sheet
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α-Cyano-4-hydroxycinnamic acid (α-Cyano-4-hydroxycinnamate) is a potent and non-competitive inhibitor of monocarboxylate transporters (MCTs). α-Cyano-4-hydroxycinnamic acid inhibits mitochondrial pyruvate transporter with a K_i of 6.3 μM. α-Cyano-4-hydroxycinnamic acid is used as a matrix to facilitate peptide ionization in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applications.

For research use only. We do not sell to patients.

CAS No. : 28166-41-8

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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Based on 11 publication(s) in Google Scholar

Other Forms of α-Cyano-4-hydroxycinnamic acid:

α-Cyano-4-hydroxycinnamic acid-d₄ Get quote
α-Cyano-4-hydroxycinnamic acid (Standard) Get quote

11 Publications Citing Use of MCE α-Cyano-4-hydroxycinnamic acid

Bio/Physico-chemical Assay

Cell Imaging/Staining

: α-Cyano-4-hydroxycinnamic acid purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2026 Feb 11;34(2):245-262.e8. [Abstract]; RAW 264.7 macrophages were infected with the WT E. faecalis and incubated in cell culture media in the presence or absence of α-Cyano-4-hydroxycinnamic acid (CHC; 5 mM) for 6 h prior to measurement of NF-kB-driven SEAP reporter activity.

: α-Cyano-4-hydroxycinnamic acid purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jul 8;14(11):4297-4317. [Abstract]; α-cyano-4-hydroxycinnamate (4-CIN; 36 mM; 3.6 μL; ICV) showed little influence on lactate production in C57BL/6 male mice. Lactate levels in the ischemic brain at 6 h after cerebral ischemia were measured.

: α-Cyano-4-hydroxycinnamic acid purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jul 8;14(11):4297-4317. [Abstract]; α-cyano-4-hydroxycinnamate (4-CIN; 36 mM; 3.6 μL; ICV) markedly reduce the protein lactylation levels of ischemic brain tissues at 6 h after cerebral ischemia in C57BL/6 male mice.

: α-Cyano-4-hydroxycinnamic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Apr;20(4):752-768. [Abstract]; Preincubation of RTH-149 cells with 100 µM α-Cyano-4-hydroxycinnamic acid (CHC) blocked the HG-induced accumulation of oxidized proteins.

: α-Cyano-4-hydroxycinnamic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Apr;20(4):752-768. [Abstract]; α-Cyano-4-hydroxycinnamic acid (CHC) prevented the formation of KFERQ-PA-mCherry1 puncta after 16 h exposure to high-glucose.

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MCT1 MCT2 MCT4

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	2 x 10² μM Compound: 116	Inhibition of EGFR in human A431 cells Inhibition of EGFR in human A431 cells	[PMID: 1479375]
A549	GI₅₀	52.7 μM Compound: 8; CHC	Antiproliferative activity against human A549 cells expressing MCT1 assessed as growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells expressing MCT1 assessed as growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36584238]
HCT-116	IC₅₀	1650 μM Compound: CHC	Cytotoxicity against human HCT-116 cells overexpressing MCT-1 assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human HCT-116 cells overexpressing MCT-1 assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 33221062]
HT-29	EC₅₀	9.8 μM Compound: CHCA	Agonist activity at GPR35 receptor in human HT-29 cells after 10 mins by dynamic mass redistribution assay Agonist activity at GPR35 receptor in human HT-29 cells after 10 mins by dynamic mass redistribution assay	[PMID: 24900447]
HT-29	IC₅₀	30.4 μM Compound: CHCA	Desensitization of GPR35 receptor in human HT-29 cells assessed as inhibition of zaprinast-induced dynamic mass redistribution after 10 mins Desensitization of GPR35 receptor in human HT-29 cells assessed as inhibition of zaprinast-induced dynamic mass redistribution after 10 mins	[PMID: 24900447]
HeLa	IC₅₀	2140 μM Compound: CHC	Cytotoxicity against human HeLa cells overexpressing MCT-1 assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human HeLa cells overexpressing MCT-1 assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 33221062]
MCF7	GI₅₀	108 μM Compound: 8; CHC	Antiproliferative activity against human MCF7 cells expressing MCT1 assessed as growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells expressing MCT1 assessed as growth inhibition incubated for 72 hrs by MTT assay	[PMID: 36584238]
Oocyte	IC₅₀	24 μM Compound: 4-CIN	TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes	[PMID: 10417314]
Oocyte	IC₅₀	425 μM Compound: 4-CIN	TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes	[PMID: 10417314]
Oocyte	IC₅₀	991 μM Compound: alpha-Cyano-4-hydroxycinnamate	TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes	[PMID: 11101640]
SiHa	EC₅₀	10.7 μM Compound: CHC	Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay	[PMID: 24095010]
SiHa	IC₅₀	10.7 μM Compound: CHC	Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting	[PMID: 24095010]
SiHa	IC₅₀	43.5 μM Compound: CHC	Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting	[PMID: 24095010]
U2OS	EC₅₀	70.7 μM Compound: CHCA	Agonist activity at GPR35 receptor in human U2OS cells coexpressing Gal4-VP16-TEV assessed as beta arrestin translocation after 5 hrs by beta lactamase reporter gene assay Agonist activity at GPR35 receptor in human U2OS cells coexpressing Gal4-VP16-TEV assessed as beta arrestin translocation after 5 hrs by beta lactamase reporter gene assay	[PMID: 24900447]

In Vitro

α-Cyano-4-hydroxycinnamic acid (α-Cyano-4-hydroxycinnamate) inhibits monocarboxylates transport such as lactate and pyruvate^[2].
α-Cyano-4-hydroxycinnamic acid (CHC; 0.5 mM and 1 mM) of 1 mM has a significant inhibitory effect on branching morphogenesis and decreases the epithelial perimeter and area of lung explants in a dose dependent way^[2].
At 100 μM concentration, α-Cyano-4-hydroxycinnamic acid rapidly and almost totally inhibits O₂ uptake by rat heart mitochondria oxidizing pyruvate. Inhibition can be detected at concentrations of inhibitor as low as 1 μM although inhibition took time to develop at this concentration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

189.17

Formula

C₁₀H₇NO₃

CAS No.

28166-41-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)/C(C#N)=C/C1=CC=C(O)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (1057.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2863 mL	26.4313 mL	52.8625 mL
5 mM	1.0573 mL	5.2863 mL	10.5725 mL
10 mM	0.5286 mL	2.6431 mL	5.2863 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (11.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (246 KB) HNMR (273 KB) RP-HPLC (248 KB)

Handling Instructions (2659 KB)

References

[1]. A P Halestrap, et al. The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors. Biochem J. 1975 Apr; 148(1): 85-96. [Content Brief]

[2]. Sara Granja, et al. The Monocarboxylate Transporter Inhibitor α-cyano-4-hydroxycinnamic Acid Disrupts Rat Lung Branching. Cell Physiol Biochem. 2013;32(6):1845-56. [Content Brief]

[3]. Makoto Watanabe, et al. Improvement of Mass Spectrometry Analysis of Glycoproteins by MALDI-MS Using 3-aminoquinoline/α-cyano-4-hydroxycinnamic Acid. Anal Bioanal Chem. 2013 May;405(12):4289-93. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.2863 mL	26.4313 mL	52.8625 mL	132.1563 mL
	5 mM	1.0573 mL	5.2863 mL	10.5725 mL	26.4313 mL
	10 mM	0.5286 mL	2.6431 mL	5.2863 mL	13.2156 mL
	15 mM	0.3524 mL	1.7621 mL	3.5242 mL	8.8104 mL
	20 mM	0.2643 mL	1.3216 mL	2.6431 mL	6.6078 mL
	25 mM	0.2115 mL	1.0573 mL	2.1145 mL	5.2863 mL
	30 mM	0.1762 mL	0.8810 mL	1.7621 mL	4.4052 mL
	40 mM	0.1322 mL	0.6608 mL	1.3216 mL	3.3039 mL
	50 mM	0.1057 mL	0.5286 mL	1.0573 mL	2.6431 mL
	60 mM	0.0881 mL	0.4405 mL	0.8810 mL	2.2026 mL
	80 mM	0.0661 mL	0.3304 mL	0.6608 mL	1.6520 mL
	100 mM	0.0529 mL	0.2643 mL	0.5286 mL	1.3216 mL

α-Cyano-4-hydroxycinnamic acid Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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α-Cyano-4-hydroxycinnamic acid (Synonyms: α-Cyano-4-hydroxycinnamate)

Customer Review

Other Forms of α-Cyano-4-hydroxycinnamic acid:

α-Cyano-4-hydroxycinnamic acid Related Antibodies

11 Publications Citing Use of MCE α-Cyano-4-hydroxycinnamic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Monocarboxylate Transporter Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

α-Cyano-4-hydroxycinnamic acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

α-Cyano-4-hydroxycinnamic acid Related Classifications

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