β-1,4-Galactosyltransferase, neisseria meningitides 

beta-1,4-Galactosyltransferase (LgtB) (EC 2.4.1.90) (B4GALT1 (LgtB)) is often used in biochemical studies. beta-1,4-Galactosyltransferase (LgtB) catalyzes the reaction involving UDP-galactose and N-acetylglucosamine for the production of galactose beta-1,4-N-acetylglucosamine^[1].

Product Information
This enzyme is used to link Gal to oligosaccharide chains with GlcNAc or Glc as non-reducing ends in a β1,4 connection. This product is recombinantly expressed in E. coli, N-terminal His Tag, molecular weight 35 kDa, storage solution components 20 mM Tris-HCl, pH 7.5, 0.5 mM NaCl, 1 mM DTT.

Instructions
1. Add substrate according to the reaction system, 5 mM MnCl₂, and appropriate buffer salts (such as Tris-HCl) can be added to adjust the pH to 6.0, and add appropriate amount of enzyme for reaction (according to the reaction requirements);
2. After mixing, incubate the sample at 37 °C for 2-4h.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed at a low speed.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9054-94-8

Liquid

Colorless to light yellow

[β-1,4-Galactosyltransferase, neisseria meningitides]

2.4.1.90

≥1 U/mL

One unit is defined as the amount of enzyme required per minute to convert 1μmol of Gal from UDP-Gal to GlcNAc to form galβ1,4GlcNAc at 37℃

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Alavi A, et al. Evaluation of beta 1,4-galactosyltransferase in rheumatoid arthritis and its role in the glycosylation network associated with this disease. Glycoconj J. 1995 Jun;12(3):206-10.  [Content Brief]