β1-3,4,6 Galactosidase 

β1-3,4,6 Galactosidase is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing β1-3 β1-4 and β1-6 linked galactose residues from oligosaccharides, with β1-6 linked galactose residues at a slower rate^[1].

others

Product Information
This product is expressed by recombinant E. coli. The buffer composition is 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol. It can be used to release β(1→3)-, β(1→4) and β(1→6)-linked galactose from the non-reducing end of complex oligosaccharides.

Instructions
1. Add substrate according to the reaction system, 50 mM sodium acetate, 5 mM CaCl2, pH 5.0, mix well, and add appropriate amount of β1-3,4,6-galactosidase (according to the reaction requirements);
2. After mixing, incubate the sample at 37°C for 2-4 h.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and low-speed shaking can be used for mixing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9031-11-2

Liquid

Colorless to light yellow

[b1-3,4,6 Galactosidase]

>2000U/mL

One unit is defined as the amount of enzyme that releases 1 nmol of galactose per minute from Gal β1-3 Glcnacβ1-3 Galβ1-4GLC at 37 °C, pH 5.0.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Andre Schutzer Godoy, et al. Crystal structure of β1→6-galactosidase from Bifidobacterium bifidum S17: trimeric architecture, molecular determinants of the enzymatic activity and its inhibition by α-galactose. FEBS J. 2016 Nov;283(22):4097-4112.  [Content Brief]