1. Anti-infection
  2. Influenza Virus
  3. 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid

2,3-Dehydro-2-deoxy-N-acetylneuraminic acid  (Synonyms: Neu5Ac2en; DANA)

Cat. No.: HY-125798 Pureza: 98.0%
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N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid (Neu5Ac2en) is a potent neuraminidase (sialidase) inhibitor. N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid shows inhibitory activities against human neuraminidase enzymes with IC50s of 143, 43, 61, and 74 μM for NEU1, NEU2, NEU3, and NEU4, respectively. Anti-influenza virus activity.

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No. CAS : 24967-27-9

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Revisión del cliente

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    2,3-Dehydro-2-deoxy-N-acetylneuraminic acid purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Oct 9.  [Abstract]

    Coimmunoprecipitation assays were performed to explore the Ub levels of exogenous Flag-tagged Nectin-4 in KU-19-19 cells with or without transfection of HA-tagged Ub and the treatment of MG132 (10 μmol/L), 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (Neu5Ac2en) (80 μmol/L), or P-3Fax-Neu5Ac (200 μmol/L). The expression and Ub levels of Flag-tagged Nectin-4 were detected by Western blot. All cell samples were treated with MG132 (10 μmol/L) before collecting cells. The results showed that after the addition of Neu5Ac2en to inhibit neuraminidases and enhance sialylation in bladder cancer cells, there was an increase in the ubiquitination levels of exogenous Nectin-4.

    2,3-Dehydro-2-deoxy-N-acetylneuraminic acid purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2023 Dec;14(6):2851-2865.  [Abstract]

    After inhibit Neu2 function by 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (DANA, 50 μM), then cultured with 15 mM lactate and differentiation for 3 days. Then detected MyHC protein level by western blot. The results showed that DANA diminished MyHC protein expression despite lactate treatment.

    2,3-Dehydro-2-deoxy-N-acetylneuraminic acid purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2023 Dec;14(6):2851-2865.  [Abstract]

    Representative photographs of MyHC, immunofluorescence staining in C2C12 cells differentiated for 3 days under L-lactate (10 mM) and 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid (DANA, 50 μM) treatment. The results showed that the growing number of myotubes induced by lactate treatment was decreased in the presence of DAN. Scale bar = 100 μm.
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    Descripciòn

    N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid (Neu5Ac2en) is a potent neuraminidase (sialidase) inhibitor. N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid shows inhibitory activities against human neuraminidase enzymes with IC50s of 143, 43, 61, and 74 μM for NEU1, NEU2, NEU3, and NEU4, respectively. Anti-influenza virus activity[1][2].

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 IC50
    168 3
    Compound: NeuAc2en
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    143 3
    Compound: 1, DANA
    Inhibition of human NEU1 expressed in HEK293 cells
    Inhibition of human NEU1 expressed in HEK293 cells
    [PMID: 18068975]
    HEK-293T IC50
    0.04 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK293 IC50
    168 3
    Compound: NeuAc2en
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    45.5 3
    Compound: NeuAc2en
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK-293T IC50
    0.06 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography
    Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography
    [PMID: 19450982]
    HEK293 IC50
    43 3
    Compound: 1, DANA
    Inhibition of human NEU2 expressed in HEK293 cells
    Inhibition of human NEU2 expressed in HEK293 cells
    [PMID: 18068975]
    HEK293 IC50
    70.3 3
    Compound: NeuAc2en
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate
    [PMID: 18694948]
    HEK-293T IC50
    0.07 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK293 IC50
    45.5 3
    Compound: NeuAc2en
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    73.1 3
    Compound: NeuAc2en
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK-293T IC50
    0.143 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK293 IC50
    61 3
    Compound: 1, DANA
    Inhibition of human NEU3 expressed in HEK293 cells
    Inhibition of human NEU3 expressed in HEK293 cells
    [PMID: 18068975]
    HEK-293T IC50
    49 3
    Compound: 1; DANA
    Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay
    Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay
    [PMID: 29425031]
    HEK-293T IC50
    0.04 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK-293T IC50
    0.06 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography
    Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography
    [PMID: 19450982]
    HEK293 IC50
    70.3 3
    Compound: NeuAc2en
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate
    [PMID: 18694948]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    0.07 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK293 IC50
    74 3
    Compound: 1, DANA
    Inhibition of human NEU4 expressed in HEK293 cells
    Inhibition of human NEU4 expressed in HEK293 cells
    [PMID: 18068975]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu2 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu2 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    0.143 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu3 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu3 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK293 IC50
    73.1 3
    Compound: NeuAc2en
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK-293T IC50
    49 3
    Compound: 1; DANA
    Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay
    Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay
    [PMID: 29425031]
    HEK-293T IC50
    49 3
    Compound: 1; DANA
    Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay
    Inhibition of human CathA-IRES-6His-tagged NEU1 expressed in HEK293T cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by fluorescence based assay
    [PMID: 29425031]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu4 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu4 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu3 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu3 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu4 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu4 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu2 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu2 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    0.143 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU1 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK293 IC50
    143 3
    Compound: 1, DANA
    Inhibition of human NEU1 expressed in HEK293 cells
    Inhibition of human NEU1 expressed in HEK293 cells
    [PMID: 18068975]
    HEK-293T IC50
    0.04 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU2 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu1 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK293 IC50
    168 3
    Compound: NeuAc2en
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu4 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu4 expressed in HEK293T cells using 4MU-Neu5Ac as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    0.06 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography
    Inhibition of human NEU3 transiently transfected in HEK293T cells by fluorimetric high-performance liquid chromatography
    [PMID: 19450982]
    HEK293 IC50
    43 3
    Compound: 1, DANA
    Inhibition of human NEU2 expressed in HEK293 cells
    Inhibition of human NEU2 expressed in HEK293 cells
    [PMID: 18068975]
    HEK-293T IC50
    50 3
    Compound: 2; DANA
    Inhibition of human Neu4 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    Inhibition of human Neu4 expressed in HEK293T cells using GM3 as substrate incubated for 30 mins by HPLC analysis
    [PMID: 27095056]
    HEK-293T IC50
    0.07 2
    Compound: 2, Neu5Ac2en, DANA
    Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis
    Inhibition of human NEU4 transiently transfected in HEK293T cells by fluorimetric analysis
    [PMID: 19450982]
    HEK293 IC50
    45.5 3
    Compound: NeuAc2en
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    61 3
    Compound: 1, DANA
    Inhibition of human NEU3 expressed in HEK293 cells
    Inhibition of human NEU3 expressed in HEK293 cells
    [PMID: 18068975]
    HEK293 IC50
    70.3 3
    Compound: NeuAc2en
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using ganglioside GM3 substrate
    [PMID: 18694948]
    HEK293 IC50
    73.1 3
    Compound: NeuAc2en
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    74 3
    Compound: 1, DANA
    Inhibition of human NEU4 expressed in HEK293 cells
    Inhibition of human NEU4 expressed in HEK293 cells
    [PMID: 18068975]
    In Vitro

    N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid (Neu5Ac2en) (10-100 μM) signifcantly inhibits sialidase activity in INS-1D cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    N-acetyl-2,3-dehydro-2-Deoxyneuraminic Acid (Neu5Ac2en) (10 mg/kg; i.p.; daily) attenuates pulmonary fibrosis in a mouse model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Mice (Mouse model of pulmonary fibrosis)[3]
    Dosage: 10 mg/kg
    Administration: I.p.; daily (starting at day 10 after Bleomycin, and then euthanized at day 21)
    Result: Inhibition of sialidases starting at day 10 after bleomycin attenuates fibrosis.
    Ensayo clínico
    Peso molecular

    291.25

    Fòrmula

    C11H17NO8

    No. CAS
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC[C@@H](O)[C@H]([C@@]1([H])[C@@H]([C@H](C=C(O1)C(O)=O)O)NC(C)=O)O

    Envío

    Room temperature in continental US; may vary elsewhere.

    Almacenamiento
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvente y solubilidad
    In Vitro: 

    H2O : 33.33 mg/mL (114.44 mM; Need ultrasonic)

    DMSO : 1 mg/mL (3.43 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4335 mL 17.1674 mL 34.3348 mL
    5 mM 0.6867 mL 3.4335 mL 6.8670 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Calculadora de molaridad

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureza y Documentación

    Purity: ≥99.0%

    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.4335 mL 17.1674 mL 34.3348 mL 85.8369 mL
    H2O 5 mM 0.6867 mL 3.4335 mL 6.8670 mL 17.1674 mL
    10 mM 0.3433 mL 1.7167 mL 3.4335 mL 8.5837 mL
    15 mM 0.2289 mL 1.1445 mL 2.2890 mL 5.7225 mL
    20 mM 0.1717 mL 0.8584 mL 1.7167 mL 4.2918 mL
    25 mM 0.1373 mL 0.6867 mL 1.3734 mL 3.4335 mL
    30 mM 0.1144 mL 0.5722 mL 1.1445 mL 2.8612 mL
    40 mM 0.0858 mL 0.4292 mL 0.8584 mL 2.1459 mL
    50 mM 0.0687 mL 0.3433 mL 0.6867 mL 1.7167 mL
    60 mM 0.0572 mL 0.2861 mL 0.5722 mL 1.4306 mL
    80 mM 0.0429 mL 0.2146 mL 0.4292 mL 1.0730 mL
    100 mM 0.0343 mL 0.1717 mL 0.3433 mL 0.8584 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Nombre del producto:
    2,3-Dehydro-2-deoxy-N-acetylneuraminic acid
    Cat. No.:
    HY-125798
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