3α-Hydroxysteroid Dehydrogenase, Microorganism  (Synonyms: 3α-HSD)

3α-Hydroxysteroid Dehydrogenase, Microorganism (3α-HSD) is an enzyme encoded by the AKR1C4 gene, which can catalyze the conversion of 3-ketosteroids into 3α-hydroxy compounds. 3α-Hydroxysteroid dehydrogenase plays an important role in the inactivation of androgen DHT, and can convert DHT into 3α-androstanediol with weak androgen activity, which can be used in the research of hirsutism^[1].

Product Information
This product is a genetically recombinant product and has no animal origin. 3α-Hydroxysteroid dehydrogenase is used clinically to catalyze the dehydrogenation reaction of hydroxysteroids and is used for the clinical detection of total bile acid.

Molecular weight: about 28 kDa (SDS-PAGE detection)
Isoelectric point: 4.8
Optimum pH: 7.0-9.0
Inhibitors: Hg²⁺, Ag⁺, etc.
Instructions
Dissolved in ddH2O at concentrations below 50 mg/mL to avoid repeated freezing and thawing. Or dissolve in buffer according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9028-56-2

Solid

White to off-white

[3a-Hydroxysteroid Dehydrogenase, Microorganism]

1.1.1.50

≥50 U/mg protein

One unit is defined as the amount of enzyme required to oxidize 1 μmol androsterone per min at pH 8.9 at 25°C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Anne Z Steiner, et al. 3alpha-Hydroxysteroid dehydrogenase type III deficiency: a novel mechanism for hirsutism. J Clin Endocrinol Metab. 2008 Apr;93(4):1298-303.   [Content Brief]