3'OMe-m7GpppAmpG  (Synonyms: m7(3'OMeG)(5')ppp(5')(2'OMeA)pG)

3’OMe-m7GpppAmpG (m7(3'OMeG)(5')ppp(5')(2'OMeA)pG) is a trinucleotide Cap1 analog with the structure m7 (3'OMeG)(5') ppp (5')(2'OMeA) pG, and also functions as a cis-acting ligase ribozyme inhibitor. 3’OMe-m7GpppAmpG effectively reduces free 5'-triphosphate groups on RNA transcripts, thereby enabling efficient co-transcriptional capping of in vitro transcribed mRNA. 3’OMe-m7GpppAmpG is not only widely used in the preparation of modified mRNA including trivalent influenza vaccine candidates, but also applicable to studies related to SARS-CoV-2 infection and other relevant research^[1][2][3][4].

3’OMe-m7GpppAmpG (4 mM; 2 h) supports the production of translationally active HA-encoding mRNA that expresses functional HA protein in HEK293 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1159.69

C₃₃H₄₅N₁₅O₂₄P₄

2089461-55-0

O[C@@H]1[C@H](OC)[C@@H](COP(OP(OP(OC[C@@H]2[C@@H](OP([O-])(OC[C@@H]3[C@@H](O)[C@@H](O)[C@H](N4C(N=C(N)NC5=O)=C5N=C4)O3)=O)[C@@H](OC)[C@H](N6C(N=CN=C7N)=C7N=C6)O2)(O)=O)(O)=O)(O)=O)O[C@H]1N8C(N=C(N)NC9=O)=C9[N+](C)=C8

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mazunina EP, et al. Trivalent mRNA vaccine-candidate against seasonal flu with cross-specific humoral immune response. Front Immunol. 2024;15:1381508. Published 2024 Apr 16.  [Content Brief]

  [2]. Su C I, et al. A cis-acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioning[J]. Nature Communications, 2024, 15(1): 6607.

  [3]. Tusup M, et al. Design of in vitro transcribed mRNA vectors for research and therapy[J]. Chimia, 2019, 73(5): 391-391.

  [4]. Kim S, et al. Innate immune responses against mRNA vaccine promote cellular immunity through IFN-β at the injection site[J]. Nature communications, 2024, 15(1): 7226.