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  4. 4-Nitrophenyl phosphate

4-Nitrophenyl phosphate  (Synonyms: p-Nitrophenyl phosphate)

Cat. No.: HY-116022 Pureza: 99.48%
COA
SDS
Instrucciones de manejo Technical Support

4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases.4-Nitrophenyl phosphate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.

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4-Nitrophenyl phosphate

4-Nitrophenyl phosphate Estructura química

No. CAS : 330-13-2

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in Water En stock
Solid
5 mg En stock
10 mg En stock
25 mg En stock
50 mg En stock
100 mg En stock
200 mg   Obtener un presupuesto  
500 mg   Obtener un presupuesto  

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Based on 3 publication(s) in Google Scholar

Other Forms of 4-Nitrophenyl phosphate:

4-Nitrophenyl phosphate Related Antibodies

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Descripciòn

4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases.4-Nitrophenyl phosphate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

In Vitro

1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Nitrophenyl phosphate Related Antibodies
Peso molecular

219.09

Fòrmula

C6H6NO6P
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

O=P(O)(O)OC(C=C1)=CC=C1[N+]([O-])=O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

H2O : 100 mg/mL (456.43 mM; Need ultrasonic)

DMSO : 100 mg/mL (456.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5643 mL 22.8217 mL 45.6433 mL
5 mM 0.9129 mL 4.5643 mL 9.1287 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación

Purity: 99.48%

SDS (393 KB)

COA (248 KB) HNMR (241 KB) LCMS (95 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.5643 mL 22.8217 mL 45.6433 mL 114.1084 mL
5 mM 0.9129 mL 4.5643 mL 9.1287 mL 22.8217 mL
10 mM 0.4564 mL 2.2822 mL 4.5643 mL 11.4108 mL
15 mM 0.3043 mL 1.5214 mL 3.0429 mL 7.6072 mL
20 mM 0.2282 mL 1.1411 mL 2.2822 mL 5.7054 mL
25 mM 0.1826 mL 0.9129 mL 1.8257 mL 4.5643 mL
30 mM 0.1521 mL 0.7607 mL 1.5214 mL 3.8036 mL
40 mM 0.1141 mL 0.5705 mL 1.1411 mL 2.8527 mL
50 mM 0.0913 mL 0.4564 mL 0.9129 mL 2.2822 mL
60 mM 0.0761 mL 0.3804 mL 0.7607 mL 1.9018 mL
80 mM 0.0571 mL 0.2853 mL 0.5705 mL 1.4264 mL
100 mM 0.0456 mL 0.2282 mL 0.4564 mL 1.1411 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Nitrophenyl phosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl330-13-2p-NitrophenylBiochemical Assay Reagentssubstrateprotein tyrosine phosphatases405 nmalkaline phosphataseALPInhibitorinhibitorinhibit

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Nombre del producto:
4-Nitrophenyl phosphate
Cat. No.:
HY-116022
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