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PNPP 

Cat. No.: HY-15928 Purity: 99.85%
Handling Instructions

PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

For research use only. We do not sell to patients.

PNPP Chemical Structure

PNPP Chemical Structure

CAS No. : 4264-83-9

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in Water USD 66 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 96 In-stock
Estimated Time of Arrival: December 31
10 g   Get quote  
50 g   Get quote  

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Description

PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

In Vitro

Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm[1].

Molecular Weight

263.05

Formula

C₆H₄NNa₂O₆P

CAS No.

4264-83-9

SMILES

O=P([O-])([O-])OC1=CC=C([N+]([O-])=O)C=C1.[Na+].[Na+]

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 160 mg/mL (608.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8016 mL 19.0078 mL 38.0156 mL
5 mM 0.7603 mL 3.8016 mL 7.6031 mL
10 mM 0.3802 mL 1.9008 mL 3.8016 mL
*Please refer to the solubility information to select the appropriate solvent.

Purity: 99.85%

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PNPP
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HY-15928
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