4-Nitrophenyl phosphate
Based on 3 publication(s) in Google Scholar
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases.4-Nitrophenyl phosphate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.
For research use only. We do not sell to patients.
- Purity: 99.48%
- CAS No.: 330-13-2
- Formula: C6H6NO6P
- Molecular Weight:219.09
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Nitrophenyl phosphate
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Biological Activity
1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 330-13-2
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Appearance Solid
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Molecular Weight 219.09
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Formula C6H6NO6P
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Color White to off-white
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SMILES
O=P(O)(O)OC(C=C1)=CC=C1[N+]([O-])=O
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Synonyms
p-Nitrophenyl phosphate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
Histone Deacetylase Inhibitors Downregulate Calcium Pyrophosphate Crystal Formation in Human Articular Chondrocytes. [Abstract]2022 Feb 26;23(5):2604. PMID: 35269745 -
Am J Physiol Cell Physiol
cGMP-dependent protein kinase II determines β-catenin accumulation that is essential for uterine decidualization in mice. [Abstract]2019 Dec 1;317(6):C1115-C1127. PMID: 31509448 -
J Chromatogr A
An in-line method for high-throughput screening of protein tyrosine phosphatase receptor type O inhibitors by capillary electrophoresis based on electrophoretically mediated microanalysis. [Abstract]2024 Jan 4:1713:464511. PMID: 38007841
Solvent & Solubility
H2O : 100 mg/mL (456.43 mM; Need ultrasonic)
DMSO : 100 mg/mL (456.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):199-205. [Content Brief]
[2]. Qi S , et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020 Jun 7;145(11):3871-3877. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.5643 mL | 22.8217 mL | 45.6433 mL | 114.1084 mL |
| 5 mM | 0.9129 mL | 4.5643 mL | 9.1287 mL | 22.8217 mL | |
| 10 mM | 0.4564 mL | 2.2822 mL | 4.5643 mL | 11.4108 mL | |
| 15 mM | 0.3043 mL | 1.5214 mL | 3.0429 mL | 7.6072 mL | |
| 20 mM | 0.2282 mL | 1.1411 mL | 2.2822 mL | 5.7054 mL | |
| 25 mM | 0.1826 mL | 0.9129 mL | 1.8257 mL | 4.5643 mL | |
| 30 mM | 0.1521 mL | 0.7607 mL | 1.5214 mL | 3.8036 mL | |
| 40 mM | 0.1141 mL | 0.5705 mL | 1.1411 mL | 2.8527 mL | |
| 50 mM | 0.0913 mL | 0.4564 mL | 0.9129 mL | 2.2822 mL | |
| 60 mM | 0.0761 mL | 0.3804 mL | 0.7607 mL | 1.9018 mL | |
| 80 mM | 0.0571 mL | 0.2853 mL | 0.5705 mL | 1.4264 mL | |
| 100 mM | 0.0456 mL | 0.2282 mL | 0.4564 mL | 1.1411 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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