4-Nitrophenyl phosphate disodium hexahydrate
Based on 2 publication(s) in Google Scholar
4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 333338-18-4
- Formula: C6H6NOP.6H2O.2Na
- Molecular Weight:371.14
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 4-Nitrophenyl phosphate disodium hexahydrate
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Biological Activity
Description
In Vitro
1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 333338-18-4
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Appearance Solid
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Molecular Weight 371.14
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Formula C6H6NOP.6H2O.2Na
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Color Off-white to light yellow
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SMILES
O=[N+]([O-])C1=CC=C(C=C1)OP(O[Na])(O[Na])=O.[6H2O]
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Synonyms
p-Nitrophenyl phosphate disodium hexahydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
Histone Deacetylase Inhibitors Downregulate Calcium Pyrophosphate Crystal Formation in Human Articular Chondrocytes. [Abstract]2022 Feb 26;23(5):2604. PMID: 35269745 -
J Chromatogr A
An in-line method for high-throughput screening of protein tyrosine phosphatase receptor type O inhibitors by capillary electrophoresis based on electrophoretically mediated microanalysis. [Abstract]2024 Jan 4:1713:464511. PMID: 38007841
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (269.44 mM; Need ultrasonic)
DMSO : 10 mg/mL (26.94 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 2):199-205. [Content Brief]
[2]. Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.6944 mL | 13.4720 mL | 26.9440 mL | 67.3600 mL |
| 5 mM | 0.5389 mL | 2.6944 mL | 5.3888 mL | 13.4720 mL | |
| 10 mM | 0.2694 mL | 1.3472 mL | 2.6944 mL | 6.7360 mL | |
| 15 mM | 0.1796 mL | 0.8981 mL | 1.7963 mL | 4.4907 mL | |
| 20 mM | 0.1347 mL | 0.6736 mL | 1.3472 mL | 3.3680 mL | |
| 25 mM | 0.1078 mL | 0.5389 mL | 1.0778 mL | 2.6944 mL | |
| H2O | 30 mM | 0.0898 mL | 0.4491 mL | 0.8981 mL | 2.2453 mL |
| 40 mM | 0.0674 mL | 0.3368 mL | 0.6736 mL | 1.6840 mL | |
| 50 mM | 0.0539 mL | 0.2694 mL | 0.5389 mL | 1.3472 mL | |
| 60 mM | 0.0449 mL | 0.2245 mL | 0.4491 mL | 1.1227 mL | |
| 80 mM | 0.0337 mL | 0.1684 mL | 0.3368 mL | 0.8420 mL | |
| 100 mM | 0.0269 mL | 0.1347 mL | 0.2694 mL | 0.6736 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
4-Nitrophenyl phosphate disodium hexahydrate Related Classifications
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