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  4. 4-Nitrophenyl phosphate disodium hexahydrate

4-Nitrophenyl phosphate (disodium hexahydrate)  (Synonyms: p-Nitrophenyl phosphate (disodium hexahydrate))

製品番号: HY-116022A 純度: 99.80%
COA 取扱説明書 Technical Support

4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.

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4-Nitrophenyl phosphate disodium hexahydrate

4-Nitrophenyl phosphate (disodium hexahydrate) 構造式

CAS 番号 : 333338-18-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
1 g $50 在庫あり
5 g $150 在庫あり
10 g $200 在庫あり
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 2 publication(s) in Google Scholar

Other Forms of 4-Nitrophenyl phosphate disodium hexahydrate:

4-Nitrophenyl phosphate (disodium hexahydrate) 関連抗体

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製品説明

4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

体外実験

1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Nitrophenyl phosphate (disodium hexahydrate) 関連抗体
分子量

371.14

分子式

C6H6NOP.6H2O.2Na
CAS 番号
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=[N+]([O-])C1=CC=C(C=C1)OP(O[Na])(O[Na])=O.[6H2O]
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外: 

H2O : 100 mg/mL (269.44 mM; Need ultrasonic)

DMSO : 10 mg/mL (26.94 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6944 mL 13.4720 mL 26.9440 mL
5 mM 0.5389 mL 2.6944 mL 5.3888 mL
10 mM 0.2694 mL 1.3472 mL 2.6944 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.80%

COA (252 KB) HNMR (252 KB) RP-HPLC (91 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.6944 mL 13.4720 mL 26.9440 mL 67.3600 mL
5 mM 0.5389 mL 2.6944 mL 5.3888 mL 13.4720 mL
10 mM 0.2694 mL 1.3472 mL 2.6944 mL 6.7360 mL
15 mM 0.1796 mL 0.8981 mL 1.7963 mL 4.4907 mL
20 mM 0.1347 mL 0.6736 mL 1.3472 mL 3.3680 mL
25 mM 0.1078 mL 0.5389 mL 1.0778 mL 2.6944 mL
H2O 30 mM 0.0898 mL 0.4491 mL 0.8981 mL 2.2453 mL
40 mM 0.0674 mL 0.3368 mL 0.6736 mL 1.6840 mL
50 mM 0.0539 mL 0.2694 mL 0.5389 mL 1.3472 mL
60 mM 0.0449 mL 0.2245 mL 0.4491 mL 1.1227 mL
80 mM 0.0337 mL 0.1684 mL 0.3368 mL 0.8420 mL
100 mM 0.0269 mL 0.1347 mL 0.2694 mL 0.6736 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Nitrophenyl phosphate disodium hexahydrate Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl phosphate disodium hexahydrate333338-18-4p-Nitrophenyl phosphate disodium hexahydrateBiochemical Assay Reagentssubstrateprotein tyrosine phosphatases405 nmalkaline phosphataseALPInhibitorinhibitorinhibit

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製品名:
4-Nitrophenyl phosphate disodium hexahydrate
製品番号:
HY-116022A
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