4-Nitrophenyl phosphate ditromethamine
Based on 2 publication(s) in Google Scholar
4-Nitrophenyl phosphate ditromethamine is an enzyme substrate commonly used in biochemical assays to measure the activity of various phosphatases, including alkaline phosphatase. 4-Nitrophenylphosphate has unique chemical properties that allow it to be hydrolyzed by phosphatases, releasing a yellow product that can be detected spectrophotometrically.
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- CAS No.: 68189-42-4
- Formula: C14H28N3O12P
- Molecular Weight:461.36
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Nitrophenyl phosphate ditromethamine
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Biological Activity
1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 68189-42-4
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Molecular Weight 461.36
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Formula C14H28N3O12P
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SMILES
O=P(O)(O)OC1=CC=C([N+]([O-])=O)C=C1.OCC(CO)(N)CO.OCC(CO)(N)CO
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Synonyms
p-Nitrophenyl phosphate ditromethamine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
Histone Deacetylase Inhibitors Downregulate Calcium Pyrophosphate Crystal Formation in Human Articular Chondrocytes. [Abstract]2022 Feb 26;23(5):2604. PMID: 35269745 -
J Chromatogr A
An in-line method for high-throughput screening of protein tyrosine phosphatase receptor type O inhibitors by capillary electrophoresis based on electrophoretically mediated microanalysis. [Abstract]2024 Jan 4:1713:464511. PMID: 38007841
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
4-Nitrophenyl phosphate ditromethamine Related Classifications
HY-116022B Related Classifications
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