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ADHP  (Synonyms: 10-Acetyl-3,7-dihydroxyphenoxazine)

Cat. No.: HY-101880 Purity: 99.90%
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ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

For research use only. We do not sell to patients.

CAS No. : 119171-73-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of ADHP:

ADHP Related Antibodies

Top Publications Citing Use of Products

    ADHP purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 May 10:696:137863.  [Abstract]

    ADHP (Amplex Red) (1 mg/mL; 5 μL; 10 s) was used to monitor the cascade enzymatic reactions in artificial cells.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

    In Vitro

    To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s 1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ADHP Related Antibodies
    Molecular Weight

    257.24

    Formula

    C14H11NO4
    CAS No.
    Appearance

    Solid

    Color

    Light brown to brown

    Emission (Em)

    585
    Excitation (Ex)

    571
    SMILES

    CC(N1C2=C(C=C(O)C=C2)OC3=CC(O)=CC=C13)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 116.67 mg/mL (453.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8874 mL 19.4371 mL 38.8742 mL
    5 mM 0.7775 mL 3.8874 mL 7.7748 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.90%

    SDS (393 KB)

    COA (248 KB) HNMR (259 KB) RP-HPLC (244 KB) LCMS (102 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    ADHP, 4-ABAH, 2-ABAH, 4-BAH, 4-FBAH, 4-NBAH, 4-TFMBAH, 3-DMABAH, NaN3 and isoniazid are dissolved in DMSO and subsequently diluted into assay buffer. The final concentration of DMSO in the reaction is less than 0.5 % (v/v), which does not affect fluorescence of the oxidized ADHP product 7-hydroxyl-3H-phenoxazin-3-one (resorufin). Reactions of ADHP (20 μM) are incubated with MPO (2.8 nm) in assay buffer and initiated by the addition of 1/10th volume H2O2 from a serial dilution basin. To determine the effect that the simplest benzoic acid hydrazide inhibitor or its analog 4-TFMBAH has on the heme catalytic ability of MPO, MPO (1.2 μM) is incubated for 10 min with different concentrations of BAH inhibitor (0, 0.025, 0.25, 2.5, 12.5 and 25 mM) with ADHP (40 μM) and timing of the reaction is measured following addition of H2O2 (20 μM) ADHP. All reactions are measured in assay buffer at room temperature. Samples of 20 μL are added to non-reducing sample loading buffers, and then loaded without prior heating and resolved by 4-15% gradient SDS-polyacrylamide gel electrophoresis[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.8874 mL 19.4371 mL 38.8742 mL 97.1855 mL
    5 mM 0.7775 mL 3.8874 mL 7.7748 mL 19.4371 mL
    10 mM 0.3887 mL 1.9437 mL 3.8874 mL 9.7186 mL
    15 mM 0.2592 mL 1.2958 mL 2.5916 mL 6.4790 mL
    20 mM 0.1944 mL 0.9719 mL 1.9437 mL 4.8593 mL
    25 mM 0.1555 mL 0.7775 mL 1.5550 mL 3.8874 mL
    30 mM 0.1296 mL 0.6479 mL 1.2958 mL 3.2395 mL
    40 mM 0.0972 mL 0.4859 mL 0.9719 mL 2.4296 mL
    50 mM 0.0777 mL 0.3887 mL 0.7775 mL 1.9437 mL
    60 mM 0.0648 mL 0.3240 mL 0.6479 mL 1.6198 mL
    80 mM 0.0486 mL 0.2430 mL 0.4859 mL 1.2148 mL
    100 mM 0.0389 mL 0.1944 mL 0.3887 mL 0.9719 mL
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    ADHP Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ADHP119171-73-210-Acetyl-3,7-dihydroxyphenoxazineFluorescent DyeInhibitorinhibitorinhibit

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