p-Nitrophenyl α-L-fucoside
Based on 1 Customer Validation
p-Nitrophenyl α-L-fucoside (PNP α-L-Fuc) is a specific substrate of Pecten maximus α-L-fucosidase, with a Km value of 650 µM. p-Nitrophenyl α-L-fucoside serves as a substrate for ClAgl29A and ClAgl29B. Enzymatic hydrolysis of p-Nitrophenyl α-L-fucoside releases p-nitrophenol and α-L-fucose, where the latter undergoes mutarotation over time to form β-L-fucose. p-Nitrophenyl α-L-fucoside is not the preferred substrate for ClAgl29A and ClAgl29B.
For research use only. We do not sell to patients.
- Purity : 98.80%
- CAS No.: 10231-84-2
- Formula: C12H15NO7
- Molecular Weight:285.25
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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BCIP
Biological Activity
Description
In Vitro
p-Nitrophenyl α-L-fucoside is weakly hydrolyzed by recombinant ClAgl29A and ClAgl29B, with kcat/Km values of 0.236 s-1 mM-1 and 0.352 s-1 mM-1, respectively. Their catalytic efficiency is much lower than that towards p-nitrophenyl α-L-glucoside[1].
p-Nitrophenyl α-L-fucoside is a specific substrate of purified Pecten maximus α-L-fucosidase, with a Km value of 650 µM. Hydrolysis of this substrate reveals that the enzyme adopts a retaining catalytic mechanism[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 10231-84-2
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Appearance Solid
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Molecular Weight 285.25
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Formula C12H15NO7
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Color White to off-white
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SMILES
O[C@@H]([C@@H]([C@@H]([C@H](C)O1)O)O)[C@@H]1OC2=CC=C([N+]([O-])=O)C=C2
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Synonyms
PNP α-L-Fuc
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (87.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 4 mg/mL (14.02 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Shishiuchi R, et al. Discovery of α-l-Glucosidase Raises the Possibility of α-l-Glucosides in Nature. ACS Omega. 2022;7(50):47411-47423. Published 2022 Dec 9. [Content Brief]
[2]. Berteau O, et al. Characterization of a new alpha-L-fucosidase isolated from the marine mollusk Pecten maximus that catalyzes the hydrolysis of alpha-L-fucose from algal fucoidan (Ascophyllum nodosum). Glycobiology. 2002;12(4):273-282. [Content Brief]
[3]. Miura T, et al. Purification and characterization of extracellular 1,2-alpha-L-fucosidase from Bacillus cereus. J Biosci Bioeng. 2005;99(6):629-635. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.5057 mL | 17.5285 mL | 35.0570 mL | 87.6424 mL |
| 5 mM | 0.7011 mL | 3.5057 mL | 7.0114 mL | 17.5285 mL | |
| 10 mM | 0.3506 mL | 1.7528 mL | 3.5057 mL | 8.7642 mL | |
| DMSO | 15 mM | 0.2337 mL | 1.1686 mL | 2.3371 mL | 5.8428 mL |
| 20 mM | 0.1753 mL | 0.8764 mL | 1.7528 mL | 4.3821 mL | |
| 25 mM | 0.1402 mL | 0.7011 mL | 1.4023 mL | 3.5057 mL | |
| 30 mM | 0.1169 mL | 0.5843 mL | 1.1686 mL | 2.9214 mL | |
| 40 mM | 0.0876 mL | 0.4382 mL | 0.8764 mL | 2.1911 mL | |
| 50 mM | 0.0701 mL | 0.3506 mL | 0.7011 mL | 1.7528 mL | |
| 60 mM | 0.0584 mL | 0.2921 mL | 0.5843 mL | 1.4607 mL | |
| 80 mM | 0.0438 mL | 0.2191 mL | 0.4382 mL | 1.0955 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
p-Nitrophenyl α-L-fucoside Related Classifications
HY-134420 Related Classifications
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