2. Metabolic Enzyme/Protease
  3. Glycosidase
  4. p-Nitrophenyl α-L-fucoside

p-Nitrophenyl α-L-fucoside  (Synonyms: PNP α-L-Fuc)

製品番号: HY-134420 純度: 98.80%
COA 取扱説明書 Technical Support

p-Nitrophenyl α-L-fucoside (PNP α-L-Fuc) is a specific substrate of Pecten maximus α-L-fucosidase, with a Km value of 650 µM. p-Nitrophenyl α-L-fucoside serves as a substrate for ClAgl29A and ClAgl29B. Enzymatic hydrolysis of p-Nitrophenyl α-L-fucoside releases p-nitrophenol and α-L-fucose, where the latter undergoes mutarotation over time to form β-L-fucose. p-Nitrophenyl α-L-fucoside is not the preferred substrate for ClAgl29A and ClAgl29B.

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p-Nitrophenyl α-L-fucoside

p-Nitrophenyl α-L-fucoside 構造式

CAS 番号 : 10231-84-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 44 在庫あり
Solution
10 mM * 1 mL in Water USD 44 在庫あり
Solid
5 mg $40 在庫あり
10 mg $63 在庫あり
25 mg $125 在庫あり
50 mg $200 在庫あり
100 mg   お問い合わせ  
200 mg   お問い合わせ  

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p-Nitrophenyl α-L-fucoside 関連抗体

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製品説明

p-Nitrophenyl α-L-fucoside (PNP α-L-Fuc) is a specific substrate of Pecten maximus α-L-fucosidase, with a Km value of 650 µM. p-Nitrophenyl α-L-fucoside serves as a substrate for ClAgl29A and ClAgl29B. Enzymatic hydrolysis of p-Nitrophenyl α-L-fucoside releases p-nitrophenol and α-L-fucose, where the latter undergoes mutarotation over time to form β-L-fucose. p-Nitrophenyl α-L-fucoside is not the preferred substrate for ClAgl29A and ClAgl29B[1][2][3].

体外実験

p-Nitrophenyl α-L-fucoside is weakly hydrolyzed by recombinant ClAgl29A and ClAgl29B, with kcat/Km values of 0.236 s-1 mM-1 and 0.352 s-1 mM-1, respectively. Their catalytic efficiency is much lower than that towards p-nitrophenyl α-L-glucoside[1].
p-Nitrophenyl α-L-fucoside is a specific substrate of purified Pecten maximus α-L-fucosidase, with a Km value of 650 µM. Hydrolysis of this substrate reveals that the enzyme adopts a retaining catalytic mechanism[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

p-Nitrophenyl α-L-fucoside 関連抗体
分子量

285.25

分子式

C12H15NO7
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@@H]([C@@H]([C@H](C)O1)O)O)[C@@H]1OC2=CC=C([N+]([O-])=O)C=C2
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (87.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 4 mg/mL (14.02 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5057 mL 17.5285 mL 35.0570 mL
5 mM 0.7011 mL 3.5057 mL 7.0114 mL
10 mM 0.3506 mL 1.7528 mL 3.5057 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

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体積 （開始）

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In Vivo Dissolution Calculator
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純度とドキュメンテーション

純度: 98.80%

COA (249 KB) HNMR (168 KB) LCMS (93 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.5057 mL 17.5285 mL 35.0570 mL 87.6424 mL
5 mM 0.7011 mL 3.5057 mL 7.0114 mL 17.5285 mL
10 mM 0.3506 mL 1.7528 mL 3.5057 mL 8.7642 mL
DMSO 15 mM 0.2337 mL 1.1686 mL 2.3371 mL 5.8428 mL
20 mM 0.1753 mL 0.8764 mL 1.7528 mL 4.3821 mL
25 mM 0.1402 mL 0.7011 mL 1.4023 mL 3.5057 mL
30 mM 0.1169 mL 0.5843 mL 1.1686 mL 2.9214 mL
40 mM 0.0876 mL 0.4382 mL 0.8764 mL 2.1911 mL
50 mM 0.0701 mL 0.3506 mL 0.7011 mL 1.7528 mL
60 mM 0.0584 mL 0.2921 mL 0.5843 mL 1.4607 mL
80 mM 0.0438 mL 0.2191 mL 0.4382 mL 1.0955 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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p-Nitrophenyl α-L-fucoside Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

p-Nitrophenyl α-L-fucoside10231-84-2PNP α-L-FucGlycosidaseC. akajimensis GH29 enzymes Caka_0506Pecten maximusα-L-glucosidases ClAgl29Bα-L-fucoseBacillus cereus no. 521p-nitrophenolα-L-glucosidases ClAgl29Aβ-L-fucoseα-L-fucosidasesC. akajimensis GH29 enzymes Caka_0074Inhibitorinhibitorinhibit

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製品名:
p-Nitrophenyl α-L-fucoside
製品番号:
HY-134420
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