4-Nitrophenyl β-D-xylopyranoside
Based on 1 Customer Validation
4-Nitrophenyl β-D-xylopyranoside is a chromogenic β-xylosidase substrate. 4-Nitrophenyl β-D-xylopyranoside can be used to test β-xylosidase activity.
For research use only. We do not sell to patients.
- Purity : 99.57%
- CAS No.: 2001-96-9
- Formula: C11H13NO7
- Molecular Weight:271.23
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Biological Activity
Description
In Vitro
β-D-xylan xylohydrolase exhibits glycosyltransferase activity with xylo-oligosaccharides and at high concentrations of 4-Nitrophenyl β-D-xylopyranoside[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2001-96-9
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Appearance Solid
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Molecular Weight 271.23
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Formula C11H13NO7
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Color White to light yellow
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SMILES
O[C@H]([C@H]([C@@H](CO1)O)O)[C@@H]1OC2=CC=C(C=C2)[N+]([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (368.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (184.35 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.6869 mL | 18.4345 mL | 36.8691 mL | 92.1727 mL |
| 5 mM | 0.7374 mL | 3.6869 mL | 7.3738 mL | 18.4345 mL | |
| 10 mM | 0.3687 mL | 1.8435 mL | 3.6869 mL | 9.2173 mL | |
| 15 mM | 0.2458 mL | 1.2290 mL | 2.4579 mL | 6.1448 mL | |
| 20 mM | 0.1843 mL | 0.9217 mL | 1.8435 mL | 4.6086 mL | |
| 25 mM | 0.1475 mL | 0.7374 mL | 1.4748 mL | 3.6869 mL | |
| 30 mM | 0.1229 mL | 0.6145 mL | 1.2290 mL | 3.0724 mL | |
| 40 mM | 0.0922 mL | 0.4609 mL | 0.9217 mL | 2.3043 mL | |
| 50 mM | 0.0737 mL | 0.3687 mL | 0.7374 mL | 1.8435 mL | |
| 60 mM | 0.0614 mL | 0.3072 mL | 0.6145 mL | 1.5362 mL | |
| 80 mM | 0.0461 mL | 0.2304 mL | 0.4609 mL | 1.1522 mL | |
| 100 mM | 0.0369 mL | 0.1843 mL | 0.3687 mL | 0.9217 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.