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  3. 4-Nitrophenyl β-D-xylopyranoside

4-Nitrophenyl β-D-xylopyranoside is a chromogenic β-xylosidase substrate. 4-Nitrophenyl β-D-xylopyranoside can be used to test β-xylosidase activity.

For research use only. We do not sell to patients.

4-Nitrophenyl β-D-xylopyranoside

4-Nitrophenyl β-D-xylopyranoside Chemical Structure

CAS No. : 2001-96-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Description

4-Nitrophenyl β-D-xylopyranoside is a chromogenic β-xylosidase substrate. 4-Nitrophenyl β-D-xylopyranoside can be used to test β-xylosidase activity[1].

In Vitro

β-D-xylan xylohydrolase exhibits glycosyltransferase activity with xylo-oligosaccharides and at high concentrations of 4-Nitrophenyl β-D-xylopyranoside[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

271.23

Formula

C11H13NO7

CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]([C@H]([C@@H](CO1)O)O)[C@@H]1OC2=CC=C(C=C2)[N+]([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (368.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (184.35 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6869 mL 18.4345 mL 36.8691 mL
5 mM 0.7374 mL 3.6869 mL 7.3738 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.57%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.6869 mL 18.4345 mL 36.8691 mL 92.1727 mL
5 mM 0.7374 mL 3.6869 mL 7.3738 mL 18.4345 mL
10 mM 0.3687 mL 1.8435 mL 3.6869 mL 9.2173 mL
15 mM 0.2458 mL 1.2290 mL 2.4579 mL 6.1448 mL
20 mM 0.1843 mL 0.9217 mL 1.8435 mL 4.6086 mL
25 mM 0.1475 mL 0.7374 mL 1.4748 mL 3.6869 mL
30 mM 0.1229 mL 0.6145 mL 1.2290 mL 3.0724 mL
40 mM 0.0922 mL 0.4609 mL 0.9217 mL 2.3043 mL
50 mM 0.0737 mL 0.3687 mL 0.7374 mL 1.8435 mL
60 mM 0.0614 mL 0.3072 mL 0.6145 mL 1.5362 mL
80 mM 0.0461 mL 0.2304 mL 0.4609 mL 1.1522 mL
100 mM 0.0369 mL 0.1843 mL 0.3687 mL 0.9217 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Product Name:
4-Nitrophenyl β-D-xylopyranoside
Cat. No.:
HY-W039938
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