4-Nitrophenyl β-D-xylopyranoside

Name: 4-Nitrophenyl β-D-xylopyranoside
Brand: MedChemExpress
SKU: HY-W039938
Rating: 4.5 (1 reviews)

Cat. No.: HY-W039938 Purity: 99.94%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-Nitrophenyl β-D-xylopyranoside is a chromogenic β-xylosidase substrate. 4-Nitrophenyl β-D-xylopyranoside can be used to test β-xylosidase activity.

For research use only. We do not sell to patients.

4-Nitrophenyl β-D-xylopyranoside Chemical Structure

CAS No. : 2001-96-9

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100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
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Description

4-Nitrophenyl β-D-xylopyranoside is a chromogenic β-xylosidase substrate. 4-Nitrophenyl β-D-xylopyranoside can be used to test β-xylosidase activity^[1].

In Vitro

β-D-xylan xylohydrolase exhibits glycosyltransferase activity with xylo-oligosaccharides and at high concentrations of 4-Nitrophenyl β-D-xylopyranoside^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

271.22

Formula

C₁₁H₁₃NO₇

CAS No.

2001-96-9

Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]([C@H]([C@@H](CO1)O)O)[C@@H]1OC2=CC=C(C=C2)[N+]([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (368.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (184.35 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6870 mL	18.4352 mL	36.8704 mL
5 mM	0.7374 mL	3.6870 mL	7.3741 mL
10 mM	0.3687 mL	1.8435 mL	3.6870 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (248 KB) HNMR (119 KB) RP-HPLC (190 KB)

Handling Instructions (2659 KB)

References

[1]. Herrmann MC, et, al. The beta-D-xylosidase of Trichoderma reesei is a multifunctional beta-D-xylan xylohydrolase. Biochem J. 1997 Jan 15;321 ( Pt 2)(Pt 2):375-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.6870 mL	18.4352 mL	36.8704 mL	92.1761 mL
	5 mM	0.7374 mL	3.6870 mL	7.3741 mL	18.4352 mL
	10 mM	0.3687 mL	1.8435 mL	3.6870 mL	9.2176 mL
	15 mM	0.2458 mL	1.2290 mL	2.4580 mL	6.1451 mL
	20 mM	0.1844 mL	0.9218 mL	1.8435 mL	4.6088 mL
	25 mM	0.1475 mL	0.7374 mL	1.4748 mL	3.6870 mL
	30 mM	0.1229 mL	0.6145 mL	1.2290 mL	3.0725 mL
	40 mM	0.0922 mL	0.4609 mL	0.9218 mL	2.3044 mL
	50 mM	0.0737 mL	0.3687 mL	0.7374 mL	1.8435 mL
	60 mM	0.0615 mL	0.3073 mL	0.6145 mL	1.5363 mL
	80 mM	0.0461 mL	0.2304 mL	0.4609 mL	1.1522 mL
	100 mM	0.0369 mL	0.1844 mL	0.3687 mL	0.9218 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.