2. Others NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Fluorescent Dye MOFs Reactive Oxygen Species (ROS)
  4. TMB

TMB  (Synonyms: BM blue; Sure Blue TMB)

Cat. No.: HY-15930 Purity: 99.98%
COA
SDS
Handling Instructions Technical Support

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.

For research use only. We do not sell to patients.

CAS No. : 54827-17-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of TMB:

TMB Related Antibodies

Top Publications Citing Use of Products

    TMB purchased from MedChemExpress. Usage Cited in: Nano Res. 2026 Feb 6.

    Ov-ZBC showed strong characteristic peaks, indicating its higher photocatalytic ROS generation capability. The results of TMB and MB chromogenic reactions shown in Fig.

    TMB purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.

    A diagram showing the chemical reaction formula of TMB assay, MB assay, and DTNB assay.

    TMB purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.

    The results of TMB assay.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].
    In Vitro

    TMB can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TMB Related Antibodies
    Molecular Weight

    240.34

    Formula

    C16H20N2
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (104.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1608 mL 20.8039 mL 41.6077 mL
    5 mM 0.8322 mL 4.1608 mL 8.3215 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    (per animal)

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.98%

    SDS (557 KB)

    COA (246 KB) HNMR (238 KB) RP-HPLC (252 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.1608 mL 20.8039 mL 41.6077 mL 104.0193 mL
    5 mM 0.8322 mL 4.1608 mL 8.3215 mL 20.8039 mL
    10 mM 0.4161 mL 2.0804 mL 4.1608 mL 10.4019 mL
    15 mM 0.2774 mL 1.3869 mL 2.7738 mL 6.9346 mL
    20 mM 0.2080 mL 1.0402 mL 2.0804 mL 5.2010 mL
    25 mM 0.1664 mL 0.8322 mL 1.6643 mL 4.1608 mL
    30 mM 0.1387 mL 0.6935 mL 1.3869 mL 3.4673 mL
    40 mM 0.1040 mL 0.5201 mL 1.0402 mL 2.6005 mL
    50 mM 0.0832 mL 0.4161 mL 0.8322 mL 2.0804 mL
    60 mM 0.0693 mL 0.3467 mL 0.6935 mL 1.7337 mL
    80 mM 0.0520 mL 0.2600 mL 0.5201 mL 1.3002 mL
    100 mM 0.0416 mL 0.2080 mL 0.4161 mL 1.0402 mL
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    TMB Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TMB54827-17-7BM blue Sure Blue TMBFluorescent DyeMOFsReactive Oxygen Species (ROS)Metal-Organic Frameworksdyestuffchromogenic agentsinglet oxygenprobeInhibitorinhibitorinhibit

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