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  4. TMB

TMB  (Synonyms: BM blue; Sure Blue TMB)

Cat. No.: HY-15930 Purity: 99.86%
COA Handling Instructions

TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

For research use only. We do not sell to patients.

TMB Chemical Structure

TMB Chemical Structure

CAS No. : 54827-17-7

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 55 In-stock
Solid
500 mg USD 50 In-stock
1 g USD 60 In-stock
5 g USD 132 In-stock
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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

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Description

TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

Molecular Weight

240.34

Appearance

Solid

Formula

C16H20N2
CAS No.
SMILES

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (416.08 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1608 mL 20.8039 mL 41.6077 mL
5 mM 0.8322 mL 4.1608 mL 8.3215 mL
10 mM 0.4161 mL 2.0804 mL 4.1608 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

TMB54827-17-7BM blue Sure Blue TMBFluorescent DyeInhibitorinhibitorinhibit

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