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Cat. No.: HY-15930R

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TMB (Standard)

(Synonyms: BM blue (Standard); Sure Blue TMB (Standard))

TMB (Standard) is the analytical standard of TMB. This product is intended for research and analytical applications. TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.

For research use only. We do not sell to patients.

CAS No.: 54827-17-7
Formula: C₁₆H₂₀N₂
Molecular Weight:240.34

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

TMB (Standard) Chemical Structure

CAS No.: 54827-17-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Product Information
- Information
- Application
Chemical Information
Purity & Documentation
Calculators
Help & FAQs
Classifications
Bioz

Product Information

Information

Application

The compound is the grade of analytical standard, which is the reference standard supplied assay. It is commonly used in qualitative, quantitative and methodological research experiments in HPLC, GC and MS.

Chemical Information

CAS No. 54827-17-7
Molecular Weight 240.34
Formula C₁₆H₂₀N₂
SMILES

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
Synonyms

BM blue (Standard); Sure Blue TMB (Standard)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

References

[1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952. [Content Brief]

[2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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