2. Others NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Fluorescent Dye Reactive Oxygen Species (ROS)
  4. TMB monosulfate

TMB monosulfate  (Synonyms: BM blue monosulfate; Sure Blue TMB monosulfate)

Cat. No.: HY-15930C Purity: 99.88%
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TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.

For research use only. We do not sell to patients.

CAS No. : 54827-18-8

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10 mM * 1 mL in DMSO
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of TMB monosulfate:

TMB monosulfate Related Antibodies

Top Publications Citing Use of Products

    TMB monosulfate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.

    A diagram showing the chemical reaction formula of TMB assay, MB assay, and DTNB assay.

    TMB monosulfate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.

    The results of TMB assay.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].
    In Vitro

    TMB monosulfate can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TMB monosulfate Related Antibodies
    Molecular Weight

    338.42

    Formula

    C16H22N2O4S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.O=S(O)(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 28 mg/mL (82.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 2.78 mg/mL (8.21 mM; ultrasonic and warming and heat to 60°C)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9549 mL 14.7745 mL 29.5491 mL
    5 mM 0.5910 mL 2.9549 mL 5.9098 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.9549 mL 14.7745 mL 29.5491 mL 73.8727 mL
    5 mM 0.5910 mL 2.9549 mL 5.9098 mL 14.7745 mL
    DMSO 10 mM 0.2955 mL 1.4775 mL 2.9549 mL 7.3873 mL
    15 mM 0.1970 mL 0.9850 mL 1.9699 mL 4.9248 mL
    20 mM 0.1477 mL 0.7387 mL 1.4775 mL 3.6936 mL
    25 mM 0.1182 mL 0.5910 mL 1.1820 mL 2.9549 mL
    30 mM 0.0985 mL 0.4925 mL 0.9850 mL 2.4624 mL
    40 mM 0.0739 mL 0.3694 mL 0.7387 mL 1.8468 mL
    50 mM 0.0591 mL 0.2955 mL 0.5910 mL 1.4775 mL
    60 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2312 mL
    80 mM 0.0369 mL 0.1847 mL 0.3694 mL 0.9234 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    TMB monosulfate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TMB monosulfate54827-18-8BM blue monosulfate Sure Blue TMB monosulfateFluorescent DyeReactive Oxygen Species (ROS)dyestuffchromogenic agentsinglet oxygenprobeInhibitorinhibitorinhibit

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