2. Others NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Fluorescent Dye Reactive Oxygen Species (ROS)
  4. TMB dihydrochloride

TMB dihydrochloride  (Synonyms: BM blue dihydrochloride; Sure Blue TMB dihydrochloride)

Cat. No.: HY-15930A Purity: 99.89%
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SDS
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TMB dihydrochloride is the dihydrochloride form of TMB (HY-15930). TMB dihydrochloride is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB dihydrochloride can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB dihydrochloride can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.

For research use only. We do not sell to patients.

CAS No. : 64285-73-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of TMB dihydrochloride:

TMB dihydrochloride Related Antibodies

Top Publications Citing Use of Products

    TMB dihydrochloride purchased from MedChemExpress. Usage Cited in: Nano Res. 2026 Feb 6.

    Ov-ZBC showed strong characteristic peaks, indicating its higher photocatalytic ROS generation capability. The results of TMB and MB chromogenic reactions shown in Fig.

    TMB dihydrochloride purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.

    A diagram showing the chemical reaction formula of TMB assay, MB assay, and DTNB assay.

    TMB dihydrochloride purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.

    The results of TMB assay.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TMB dihydrochloride is the dihydrochloride form of TMB (HY-15930). TMB dihydrochloride is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB dihydrochloride can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB dihydrochloride can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].
    In Vitro

    TMB dihydrochloride can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TMB dihydrochloride Related Antibodies
    Molecular Weight

    313.27

    Formula

    C16H22Cl2N2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[2HCl]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 11.67 mg/mL (37.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 11.11 mg/mL (35.46 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1921 mL 15.9607 mL 31.9213 mL
    5 mM 0.6384 mL 3.1921 mL 6.3843 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.89%

    SDS (396 KB)

    COA (248 KB) LCMS (103 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.1921 mL 15.9607 mL 31.9213 mL 79.8034 mL
    5 mM 0.6384 mL 3.1921 mL 6.3843 mL 15.9607 mL
    10 mM 0.3192 mL 1.5961 mL 3.1921 mL 7.9803 mL
    15 mM 0.2128 mL 1.0640 mL 2.1281 mL 5.3202 mL
    20 mM 0.1596 mL 0.7980 mL 1.5961 mL 3.9902 mL
    25 mM 0.1277 mL 0.6384 mL 1.2769 mL 3.1921 mL
    30 mM 0.1064 mL 0.5320 mL 1.0640 mL 2.6601 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    TMB dihydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TMB64285-73-0BM blueSure Blue TMBFluorescent DyeReactive Oxygen Species (ROS)dyestuffchromogenic agentsinglet oxygenprobeInhibitorinhibitorinhibit

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