TMB dihydrochloride x.hydrate
Based on 8 publication(s) in Google Scholar
TMB (dihydrochloride x.hydrate) is the dihydrochloride x hydrate form of TMB (HY-15930). TMB (dihydrochloride x.hydrate) is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB (dihydrochloride x.hydrate) can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB (dihydrochloride x.hydrate) can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 207738-08-7
- Formula: C16H22Cl2N2.xH2O
-
Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
Publications Citing Use of MedChemExpress (MCE) TMB dihydrochloride x.hydrate
More-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
Biological Activity
TMB (dihydrochloride x.hydrate) can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 207738-08-7
-
Appearance Solid
-
Formula C16H22Cl2N2.xH2O
-
Color Off-white to light brown
-
SMILES
NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[x].Cl.Cl.O
-
Synonyms
Sure Blue TMB dihydrochloride x.hydrate
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
TMB dihydrochloride x.hydrate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.
A diagram showing the chemical reaction formula of TMB assay, MB assay, and DTNB assay.
TMB dihydrochloride x.hydrate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.
The results of TMB assay.
-
J Control Release
Synergistic photothermal-sonodynamic therapy for antibacterial and immune reprogramming in chronic osteomyelitis. [Abstract]2025 May 10:381:113612. PMID: 40073945 -
TMB dihydrochloride x.hydrate purchased from MedChemExpress. Usage Cited in: Nano Res. 2026 Feb 6.
Ov-ZBC showed strong characteristic peaks, indicating its higher photocatalytic ROS generation capability. The results of TMB and MB chromogenic reactions shown in Fig.
-
Int J Biol Macromol
Construction and characterization of a novel single-chain variable fragment against Bacillus thuringiensis vegetative insecticidal protein. [Abstract]2025 Sep 14;328(Pt 1):147690. PMID: 40957516 -
-
-
J Invertebr Pathol
Characterization of the individual domains of the Bacillus thuringiensis Cry2Aa implicates Domain I as a possible binding site to Helicoverpa armigera. [Abstract]2024 Jul:205:108129. PMID: 38754546 -
Solvent & Solubility
DMSO : 10 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
[1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952. [Content Brief]
[2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)