TMB dihydrochloride x.hydrate
Based on 8 publication(s) in Google Scholar
TMB (dihydrochloride x.hydrate) is the dihydrochloride x hydrate form of TMB (HY-15930). TMB (dihydrochloride x.hydrate) is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB (dihydrochloride x.hydrate) can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB (dihydrochloride x.hydrate) can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
For research use only. We do not sell to patients.
- Purity : 99.89%
- CAS No.: 207738-08-7
- Formula: C16H22Cl2N2.xH2O
-
Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-15930
TMBChromogenic Agent -
HY-15922
LuminolChemiluminescent Substance -
HY-15930R
TMB (Standard)Chromogenic Agent -
HY-15930A
TMB dihydrochlorideChromogenic Agent -
HY-A0070A
LiothyronineTIGIT/PVR Blocker -
HY-D0083
DiIFluorescent Dye -
HY-15909
BCIPChromogenic Substrate -
HY-B0099
EdaravoneApoptosis Inducer -
HY-A0070
Liothyronine sodiumTRα/TRβ Agonist -
HY-15915
DTNBThiol Reactant -
HY-12591B
D-Luciferin potassiumSubstrate for Luciferase -
HY-W010696
Reverse T3Thyroid Hormone
Publications Citing Use of MedChemExpress (MCE) TMB dihydrochloride x.hydrate
More
Customer Validation & Images
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
Biological Activity
Description
In Vitro
TMB (dihydrochloride x.hydrate) can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 207738-08-7
-
Appearance Solid
-
Formula C16H22Cl2N2.xH2O
-
Color Off-white to light brown
-
SMILES
NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[x].Cl.Cl.O
-
Synonyms
Sure Blue TMB dihydrochloride x.hydrate
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
TMB dihydrochloride x.hydrate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.
A diagram showing the chemical reaction formula of TMB assay, MB assay, and DTNB assay.
TMB dihydrochloride x.hydrate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.
The results of TMB assay.
-
J Control Release
Synergistic photothermal-sonodynamic therapy for antibacterial and immune reprogramming in chronic osteomyelitis. [Abstract]2025 May 10:381:113612. PMID: 40073945 -
TMB dihydrochloride x.hydrate purchased from MedChemExpress. Usage Cited in: Nano Res. 2026 Feb 6.
Ov-ZBC showed strong characteristic peaks, indicating its higher photocatalytic ROS generation capability. The results of TMB and MB chromogenic reactions shown in Fig.
-
Int J Biol Macromol
Construction and characterization of a novel single-chain variable fragment against Bacillus thuringiensis vegetative insecticidal protein. [Abstract]2025 Sep 14;328(Pt 1):147690. PMID: 40957516 -
-
-
J Invertebr Pathol
Characterization of the individual domains of the Bacillus thuringiensis Cry2Aa implicates Domain I as a possible binding site to Helicoverpa armigera. [Abstract]2024 Jul:205:108129. PMID: 38754546 -
Solvent & Solubility
In Vitro:
DMSO : 10 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Protocols
-
ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
-
Genotoxicity/Mutagenicity Study
The bacterial reverse mutation assay detects point mutations that restore amino-acid prototrophy in auxotrophic Salmonella typhimurium or Escherichia coli tester strains; after exposure to a test article, mutagenic activity is read out as an increased number of revertant colonies on minimal agar compared with the vehicle control. The assay uses tester strains with different mutation targets so that base-substitution and frameshift mutagens can be detected, and testing is performed with and without exogenous mammalian metabolic activation because some chemicals require biotransformation to become mutagenic.
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
[1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952. [Content Brief]
[2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TMB dihydrochloride x.hydrate Related Classifications
HY-15930B Related Classifications
Powered by Bioz