4-Nitrophenyl β-D-Cellobioside
Based on 1 Customer Validation
4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog. 4-Nitrophenyl β-D-Cellobioside is hydrolyzed by β-glucosidases such as TxGH116 and ThCel7B. 4-Nitrophenyl β-D-Cellobioside can also be hydrolyzed by exoglucanases and endoglucanases to produce p-nitrophenol (PNP). 4-Nitrophenyl β-D-Cellobioside can be used to detect cellulase activity.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 3482-57-3
- Formula: C18H25NO13
- Molecular Weight:463.39
-
Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Chemical Information
-
CAS No. 3482-57-3
-
Appearance Solid
-
Molecular Weight 463.39
-
Formula C18H25NO13
-
Color White to light yellow
-
SMILES
O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)O)[C@@H]1OC3=CC=C([N+]([O-])=O)C=C3
-
Synonyms
p-Nitrophenyl β-D-Cellobioside
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 5 mg/mL (10.79 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53. [Content Brief]
[3]. Charoenwattanasatien R, et al. Bacterial β-Glucosidase Reveals the Structural and Functional Basis of Genetic Defects in Human Glucocerebrosidase 2 (GBA2). ACS Chem Biol. 2016 Jul 15;11(7):1891-900. [Content Brief]
[4]. Kudo K, et al. Purification and enzymatic characterization of secretory glycoside hydrolase family 3 (GH3) aryl β-glucosidases screened from Aspergillus oryzae genome. J Biosci Bioeng. 2015 Dec;120(6):614-23. [Content Brief]
[5]. Pellegrini VO, et al. Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme. Appl Microbiol Biotechnol. 2015 Nov;99(22):9591-604. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.1580 mL | 10.7900 mL | 21.5801 mL | 53.9502 mL |
| 5 mM | 0.4316 mL | 2.1580 mL | 4.3160 mL | 10.7900 mL | |
| 10 mM | 0.2158 mL | 1.0790 mL | 2.1580 mL | 5.3950 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
4-Nitrophenyl β-D-Cellobioside Related Classifications
HY-137827 Related Classifications
Powered by Bioz