3482-57-3
Chemical Structure
4-Nitrophenyl β-D-Cellobioside
Synonym(s): p-Nitrophenyl β-D-Cellobioside
- CAS No.: 3482-57-3
- Formula:C18H25NO13
- Molecular Weight:463.39
IUPAC Name: (2S,3R,4S,5S,6R)-2-(((2R,3S,4R,5R,6S)-4,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)tetrahydro-2H-pyran-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
InChIKey: IAYJZWFYUSNIPN-KFRZSCGFSA-N
SMILES: O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)O)[C@@H]1OC3=CC=C([N+]([O-])=O)C=C3
Biological Activity: 4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog. 4-Nitrophenyl β-D-Cellobioside is hydrolyzed by β-glucosidases such as TxGH116 and ThCel7B. 4-Nitrophenyl β-D-Cellobioside can also be hydrolyzed by exoglucanases and endoglucanases to produce p-nitrophenol (PNP). 4-Nitrophenyl β-D-Cellobioside can be used to detect cellulase activity[1][2][31][4][5].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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4-Nitrophenyl β-D-Cellobioside | 99.94% | 4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog. 4-Nitrophenyl β-D-Cellobioside is hydrolyzed by β-glucosidases such as TxGH116 and ThCel7B. 4-Nitrophenyl β-D-Cellobioside can also be hydrolyzed by exoglucanases and endoglucanases to produce p-nitrophenol (PNP). 4-Nitrophenyl β-D-Cellobioside can be used to detect cellulase activity. | ||||||||||||||||||||
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References
- [1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53. [Content Brief]
- [2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31.
- [4]. Kudo K, et al. Purification and enzymatic characterization of secretory glycoside hydrolase family 3 (GH3) aryl β-glucosidases screened from Aspergillus oryzae genome. J Biosci Bioeng. 2015 Dec;120(6):614-23. [Content Brief]
- [5]. Pellegrini VO, et al. Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme. Appl Microbiol Biotechnol. 2015 Nov;99(22):9591-604. [Content Brief]
