1. Others Metabolic Enzyme/Protease
  2. Biochemical Assay Reagents Glycosidase
  3. p-Nitrophenyl β-D-lactoside

p-Nitrophenyl β-D-lactoside is a substrate of β-lactosidase and can be used to determine β-lactosinase activity. p-Nitrophenyl β-D-lactoside is a substrate of cellobiohydrolase. p-Nitrophenyl β-D-lactoside is a class of biochemical reagents used in glycobiology research.

For research use only. We do not sell to patients.

p-Nitrophenyl β-D-lactoside

p-Nitrophenyl β-D-lactoside Chemical Structure

CAS No. : 4419-94-7

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Description

p-Nitrophenyl β-D-lactoside is a substrate of β-lactosidase and can be used to determine β-lactosinase activity. p-Nitrophenyl β-D-lactoside is a substrate of cellobiohydrolase. p-Nitrophenyl β-D-lactoside is a class of biochemical reagents used in glycobiology research[1][2].

Molecular Weight

463.39

Formula

C18H25NO13

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H](O[C@H]([C@@H]([C@H]1O)O)OC2=CC=C(C=C2)[N+]([O-])=O)[C@H]1O[C@@H]3O[C@@H]([C@@H]([C@@H]([C@H]3O)O)O)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

H2O : 4.17 mg/mL (9.00 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1580 mL 10.7900 mL 21.5801 mL
5 mM 0.4316 mL 2.1580 mL 4.3160 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.1580 mL 10.7900 mL 21.5801 mL 53.9502 mL
5 mM 0.4316 mL 2.1580 mL 4.3160 mL 10.7900 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
p-Nitrophenyl β-D-lactoside
Cat. No.:
HY-137812
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