5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside
Based on 1 Customer Validation
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, a chromogenic substrate for the detection of β-galactosidase activity. It is commonly used in molecular biology techniques such as gene expression analysis and reporter gene analysis. When β-galactosidase cleaves X-Gluc, a blue precipitate is produced, which can be observed by microscopy or other detection methods. X-Gluc has high sensitivity and specificity for the detection of β-galactosidase activity, making it a widely used tool in molecular biology research.
For research use only. We do not sell to patients.
- Purity : 99.11%
- CAS No.: 15548-60-4
- Formula: C14H15BrClNO6
- Molecular Weight:408.63
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
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HY-15934
X-GAL -
HY-DY2002
X-GAL (solution) -
HY-W011411
4-Nitrophenyl a-D-glucopyranoside -
HY-W039938
4-Nitrophenyl β-D-xylopyranoside -
HY-15935C
X-Gluc sodium -
HY-N2325
D-(+)-Cellobiose -
HY-15909
BCIP -
HY-12591A
D-Luciferin -
HY-N2325R
D-(+)-Cellobiose (Standard) -
HY-15927
4-Nitrophenyl β-D-glucopyranoside -
HY-134420
p-Nitrophenyl α-L-fucoside -
HY-15935B
X-Gluc cyclohexanamine
Biological Activity
Description
In Vitro
5-Bromo-4-chloro-3-indolyl β-D-Glucopyranoside is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 15548-60-4
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Appearance Solid
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Molecular Weight 408.63
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Formula C14H15BrClNO6
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Color White to off-white
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SMILES
ClC1=C2C(O[C@@H]([C@@H]([C@H]3O)O)O[C@@H]([C@H]3O)CO)=CNC2=CC=C1Br
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (244.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (250 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4472 mL | 12.2360 mL | 24.4720 mL | 61.1800 mL |
| 5 mM | 0.4894 mL | 2.4472 mL | 4.8944 mL | 12.2360 mL | |
| 10 mM | 0.2447 mL | 1.2236 mL | 2.4472 mL | 6.1180 mL | |
| 15 mM | 0.1631 mL | 0.8157 mL | 1.6315 mL | 4.0787 mL | |
| 20 mM | 0.1224 mL | 0.6118 mL | 1.2236 mL | 3.0590 mL | |
| 25 mM | 0.0979 mL | 0.4894 mL | 0.9789 mL | 2.4472 mL | |
| 30 mM | 0.0816 mL | 0.4079 mL | 0.8157 mL | 2.0393 mL | |
| 40 mM | 0.0612 mL | 0.3059 mL | 0.6118 mL | 1.5295 mL | |
| 50 mM | 0.0489 mL | 0.2447 mL | 0.4894 mL | 1.2236 mL | |
| 60 mM | 0.0408 mL | 0.2039 mL | 0.4079 mL | 1.0197 mL | |
| 80 mM | 0.0306 mL | 0.1530 mL | 0.3059 mL | 0.7648 mL | |
| 100 mM | 0.0245 mL | 0.1224 mL | 0.2447 mL | 0.6118 mL |
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside Related Classifications
HY-137779 Related Classifications
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