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X-GAL  (Synonyms: BCIG)

Cat. No.: HY-15934 Purity: 99.88%
COA Handling Instructions

X-GAL is a widely used chromogenic β-galactosidase substrate. β-galactosidase cleaves X-gal and produce an insoluble blue compound, which is detectable.

For research use only. We do not sell to patients.

X-GAL Chemical Structure

X-GAL Chemical Structure

CAS No. : 7240-90-6

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1 g USD 180 In-stock
2 g USD 250 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

X-GAL Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE X-GAL

  • Biological Activity

  • Purity & Documentation

  • Customer Review

Description

X-GAL is a widely used chromogenic β-galactosidase substrate. β-galactosidase cleaves X-gal and produce an insoluble blue compound, which is detectable.

Molecular Weight

408.63

Appearance

Solid

Formula

C14H15BrClNO6
CAS No.
SMILES

O[C@H]([C@@H](O)[C@H]1O)[C@@H](O[C@@H]1CO)OC2=CNC3=C2C(Cl)=C(Br)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMF : 200 mg/mL (489.44 mM; Need ultrasonic)

DMSO : 100 mg/mL (244.72 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4472 mL 12.2360 mL 24.4720 mL
5 mM 0.4894 mL 2.4472 mL 4.8944 mL
10 mM 0.2447 mL 1.2236 mL 2.4472 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMF    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2 mg/mL (4.89 mM); Clear solution

  • 5.

    Add each solvent one by one:  10% DMF    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2 mg/mL (4.89 mM); Clear solution

  • 6.

    Add each solvent one by one:  10% DMF    90% Corn Oil

    Solubility: ≥ 2 mg/mL (4.89 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.88%

COA (249 KB) HNMR (188 KB) LCMS (484 KB)

Handling Instructions (2659 KB)
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X-GAL Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

X-GAL7240-90-6BCIGFluorescent DyeInhibitorinhibitorinhibit

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