1. Biochemical Assay Reagents

IPTG (Synonyms: Isopropyl β-D-thiogalactoside)

Cat. No.: HY-15921 Purity: >98.0%
Data Sheet SDS Handling Instructions

IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

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IPTG Chemical Structure

IPTG Chemical Structure

CAS No. : 367-93-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $55 In-stock
1 g $50 In-stock
5 g $80 In-stock
10 g   Get quote  
50 g   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

In Vitro

IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 4.1964 mL 20.9820 mL 41.9639 mL
5 mM 0.8393 mL 4.1964 mL 8.3928 mL
10 mM 0.4196 mL 2.0982 mL 4.1964 mL
Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

238.3

Formula

C₉H₁₈O₅S

CAS No.

367-93-1

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 60 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: >98.0%

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Product Name:
IPTG
Cat. No.:
HY-15921
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