Adenosine monophosphate
Based on 19 publication(s) in Google Scholar
Adenosine monophosphate is an adenosine A1 receptor agonist. Adenosine monophosphate has significant antiviral activity against HSV-1 and HSV-2. Adenosine monophosphate is a key cellular metabolite regulating energy homeostasis and signal transduction.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 61-19-8
- Formula: C10H14N5O7P
- Molecular Weight:347.22
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Adenosine monophosphate
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Cell. 2025 Oct 30;188(22):6151-6169.e24. [Abstract]
- Theranostics. 2024 Aug 12;14(12):4874-4893. [Abstract]
- Theranostics. 2020 Aug 13;10(22):10245-10261. [Abstract]
- MedComm (2020). 2025 Jan 8;6(1):e70046. [Abstract]
- J Exp Med. 2026 Jan 5;223(1):e20250440. [Abstract]
- Stem Cell Res Ther. 2025 Jun 2;16(1):277. [Abstract]
- Stem Cell Res Ther. 2025 Mar 26;16(1):148. [Abstract]
- Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]
- Anal Chem. 2025 May 13;97(18):9827-9835. [Abstract]
- Mar Pollut Bull. 2025 Nov:220:118455. [Abstract]
- Molecules. 2025 Mar 9;30(6):1224. [Abstract]
- Molecules. 2023 Apr 11;28(8):3375. [Abstract]
- Genes (Basel). 2022 Dec 16;13(12):2384. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]
- Res Sq. 2026 Apr 22:rs.3.rs-9192680. [Abstract]
- University of Cambridge. 2023 Feb.
- Research Square Preprint. 2022 May.
Customer Validation & Images
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Flow Cytometry
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Cell Imaging/Staining
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WB
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ELISA
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Flow Cytometry
All Endogenous Metabolite Isoforms
MoreAll Adenosine Receptor Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
Microbial Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | EC50 |
>100 μM
Compound: AMP
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Cytotoxicity against human HCT-116 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-116 cells incubated for 48 hrs by MTT assay
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[PMID: 36518704] |
| HCT-116 | EC50 |
>100 μM
Compound: AMP
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Cytotoxicity against human HCT-116 cells overexpressing human BCRP incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-116 cells overexpressing human BCRP incubated for 48 hrs by MTT assay
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[PMID: 36518704] |
| HCT-116 | EC50 |
>100 μM
Compound: AMP
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Cytotoxicity against human HCT-116 cells overexpressing human MDR1 incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-116 cells overexpressing human MDR1 incubated for 48 hrs by MTT assay
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[PMID: 36518704] |
| HEK293 | EC50 |
>100 μM
Compound: AMP
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Cytotoxicity against human HEK293 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human HEK293 cells incubated for 48 hrs by MTT assay
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[PMID: 36518704] |
| HEK-293T | EC50 |
0.5 μM
Compound: 5'-AMP
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Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay
Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay
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[PMID: 22738238] |
| HepG2 | EC50 |
1.4 μM
Compound: AMP
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Allosteric activation of AMPK alpha2/beta1/gamma1 in human HepG2 cells by scintillation proximity assay
Allosteric activation of AMPK alpha2/beta1/gamma1 in human HepG2 cells by scintillation proximity assay
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[PMID: 31744674] |
Adenosine monophosphate is an adenosine A1 receptor agonist[1]. Adenosine monophosphate-induced cell toxicity is negligible at concentrations of 25 to 400 μM in RAW264.7 cells. Adenosine monophosphate significantly attenuates the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in RAW264.7 cells. The dose-dependence of TNF-α and IL-6 mRNA show that at concentration of 400 μM, Adenosine monophosphate exhibits the maximum inhibition. Exposure of cells to Adenosine monophosphate significantly reduces recruitment of NF-κB p65 to TNF-α, IL-6, and IL-1β gene promoters[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 61-19-8
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Appearance Solid
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Molecular Weight 347.22
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Formula C10H14N5O7P
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Color White to off-white
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SMILES
O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C=NC3=C2N=CN=C3N
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Synonyms
AMP
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (19)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Cell
Adenosine kinase and ADAL coordinate detoxification of modified adenosines to safeguard metabolism. [Abstract]2025 Oct 30;188(22):6151-6169.e24. PMID: 40840445 -
Theranostics
Engaging natural regulatory myeloid cells to restrict T-cell hyperactivation-induced liver inflammation via extracellular vesicle-mediated purine metabolism regulation. [Abstract]2024 Aug 12;14(12):4874-4893. PMID: 39239508 -
Theranostics
Histone deacetylase inhibitors promote epithelial-mesenchymal transition in Hepatocellular Carcinoma via AMPK-FOXO1-ULK1 signaling axis-mediated autophagy. [Abstract]2020 Aug 13;10(22):10245-10261. PMID: 32929346 -
MedComm (2020)
Novel antibiotics against Staphylococcus aureus without detectable resistance by targeting proton motive force and FtsH. [Abstract]2025 Jan 8;6(1):e70046. PMID: 39781293 -
J Exp Med
2026 Jan 5;223(1):e20250440. PMID: 41165618 -
Stem Cell Res Ther
CD73-expressing endometrial regenerative cell-derived exosomes mitigate acute cardiac allograft rejection through regulating adenosine metabolism in mice. [Abstract]2025 Jun 2;16(1):277. PMID: 40457389
Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Jun 2;16(1):277. [Abstract]
Flow cytometry analysis of adenosine A2aR and A2bR expression treated with Adenosine monophosphate (AMP) (100 μM).
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Stem Cell Res Ther
Exosomes derived from a mesenchymal-like endometrial regenerative cells ameliorate renal ischemia reperfusion injury through delivery of CD73. [Abstract]2025 Mar 26;16(1):148. PMID: 40140882 -
Stem Cell Res Ther
CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells. [Abstract]2023 Sep 29;14(1):277. PMID: 37775797
Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]
IFN-γ production was detected by ELISA in the cell culture supernatant, ERCs or NC-ERCs significantly inhibited the differentiation of activated CD4+ T cells to Th1 cells compared with CD73-KO-ERCs, as well as the production of IFN-γ, especially in the presence of Adenosine monophosphate (AMP) (50 μM, 72 h).
Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]
Adenosine monophosphate (AMP) (50 μM, 72 h) was introduced to the culture medium to ensure sufficient substrate for CD73 in all situations, the reduction of CD69 expression on CD4+ T cells was more apparent when cultured with NC-ERCs than CD73-KO-ERCs.
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Anal Chem
Ultrahigh Flow Regulated Discharge Coupling with Targeted Mass Spectrometry Analysis: Real Time Capturing Biomolecules in Exhaled Breath. [Abstract]2025 May 13;97(18):9827-9835. PMID: 40302637 -
Mar Pollut Bull
Microplastics exposure affects the feeding behavior of the whiteleg shrimp Litopenaeus vannamei by impairing the appetite, olfaction and digestion. [Abstract]2025 Nov:220:118455. PMID: 40680651 -
Molecules
Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]2025 Mar 9;30(6):1224. PMID: 40142001 -
Molecules
Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]2023 Apr 11;28(8):3375. PMID: 37110608 -
Genes (Basel)
2022 Dec 16;13(12):2384. PMID: 36553651
Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Genes (Basel). 2022 Dec 16;13(12):2384. [Abstract]
The Survival rate of M. coruscus larvae after being treated with Adenosine monophosphate (AMP) (0.5, 1. 2.5 mM, 72 h) The figures in the legend represent the concentration (mM).
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Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Biochem Biophys Res Commun
Feedback activation of CD73-Adenosine axis attenuates the antitumor immunity of STING pathway. [Abstract]2024 May 14:708:149814. PMID: 38531218
Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]
The levels of ROS was significantly improved in the AMPD2 overexpression group after treatment with Adenosine monophosphate (AMP) (10 μM, 24 h).
Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]
Lower expression of total p38, phosphorylated p38 and Gpx4 could be observed after Adenosine monophosphate (AMP) (10 μM, 24 h) treatment in the AMPD2 overexpression group detected by western blot analysis.
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Res Sq
A phosphorylation switch at MRE11 links ATM-ATR and calcium signaling to safeguard stalled replication fork stability. [Abstract]2026 Apr 22:rs.3.rs-9192680. PMID: 42078910 -
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Solvent & Solubility
DMSO : 16.67 mg/mL (48.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1.67 mg/mL (4.81 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 3.33 mg/mL (9.59 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Rittiner JE, et al. AMP is an adenosine A1 receptor agonist. J Biol Chem. 2012 Feb 17;287(8):5301-9. [Content Brief]
[2]. Zhan Y, et al. Adenosine 5'-monophosphate ameliorates D-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice. Cell Death Dis. 2014 Jan 9;5:e985. [Content Brief]
[3]. Ayisi NK, et al. Comparison of the antiviral effects of 5-methoxymethyldeoxyuridine-5'-monophosphate with adenine arabinoside-5'-monophosphate. Antiviral Res. 1983 Sep;3(3):161-74. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.8800 mL | 14.3999 mL | 28.7999 mL | 71.9996 mL |
| DMSO | 5 mM | 0.5760 mL | 2.8800 mL | 5.7600 mL | 14.3999 mL |
| 10 mM | 0.2880 mL | 1.4400 mL | 2.8800 mL | 7.2000 mL | |
| 15 mM | 0.1920 mL | 0.9600 mL | 1.9200 mL | 4.8000 mL | |
| 20 mM | 0.1440 mL | 0.7200 mL | 1.4400 mL | 3.6000 mL | |
| 25 mM | 0.1152 mL | 0.5760 mL | 1.1520 mL | 2.8800 mL | |
| 30 mM | 0.0960 mL | 0.4800 mL | 0.9600 mL | 2.4000 mL | |
| 40 mM | 0.0720 mL | 0.3600 mL | 0.7200 mL | 1.8000 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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