Adenosine 5'-monophosphate (monohydrate)
Based on 22 publication(s) in Google Scholar
Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist. Adenosine 5'-monophosphate monohydrate has significant antiviral activity against HSV-1 and HSV-2.
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- 純度: 99.81%
- CAS 番号: 18422-05-4
- 分子式: C10H16N5O8P
- 分子量:365.24
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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MedChemExpress（MCE）の使用を引用している文献 Adenosine 5'-monophosphate monohydrate
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Cell. 2025 Oct 30;188(22):6151-6169.e24. [Abstract]
- Exploration. 2026 Jun 22. [Abstract]
- Theranostics. 2024 Aug 12;14(12):4874-4893. [Abstract]
- Theranostics. 2020 Aug 13;10(22):10245-10261. [Abstract]
- MedComm (2020). 2025 Jan 8;6(1):e70046. [Abstract]
- J Exp Med. 2026 Jan 5;223(1):e20250440. [Abstract]
- Cell Chem Biol. 2026 Jun 18;33(6):767-784.e7. [Abstract]
- Stem Cell Res Ther. 2025 Jun 2;16(1):277. [Abstract]
- Stem Cell Res Ther. 2025 Mar 26;16(1):148. [Abstract]
- Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]
- Anal Chem. 2025 May 13;97(18):9827-9835. [Abstract]
- Biochem Pharmacol. 2026 Sep;251(Pt 1):118080. [Abstract]
- Molecules. 2025 Mar 9;30(6):1224. [Abstract]
- Molecules. 2023 Apr 11;28(8):3375. [Abstract]
- Mar Pollut Bull. 2025 Nov:220:118455. [Abstract]
- Genes (Basel). 2022 Dec 16;13(12):2384. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]
- Res Sq. 2026 Apr 22:rs.3.rs-9192680. [Abstract]
- University of Cambridge. 2023 Feb.
- Research Square Preprint. 2022 May.
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Endogenous Metabolite アイソフォーム固有の製品をすべて表示
MoreAdenosine Receptor アイソフォーム固有の製品をすべて表示
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生物活性
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Microbial Metabolite |
HSV-1 |
HSV-2 |
Human Endogenous Metabolite |
Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist[1]. Adenosine 5'-monophosphate monohydrate (5′-AMP)-induced cell toxicity is negligible at concentrations of 25 to 400 μM in RAW264.7 cells. Adenosine 5'-monophosphate monohydrate significantly attenuates the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in RAW264.7 cells. The dose-dependence of TNF-α and IL-6 mRNA show that at concentration of 400 μM, Adenosine 5'-monophosphate monohydrate exhibits the maximum inhibition. Exposure of cells to Adenosine 5'-monophosphate monohydrate significantly reduces recruitment of NF-κB p65 to TNF-α, IL-6, and IL-1β gene promoters[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
化学情報
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CAS 番号 18422-05-4
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性状 Solid
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分子量 365.24
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分子式 C10H16N5O8P
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Color White to off-white
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SMILES
O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C=NC3=C2N=CN=C3N.O
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別名
5'-AMP (monohydrate); アデノシン 5′-一リン酸 一水和物
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (22)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Cell
Adenosine kinase and ADAL coordinate detoxification of modified adenosines to safeguard metabolism. [Abstract]2025 Oct 30;188(22):6151-6169.e24. PMID: 40840445 -
Exploration
Radiolabeled Coordination Polymer-Loaded Microneedles for Synergistic Melanoma Brachytherapy-Immunotherapy via STING Activation and Pyroptosis. [Abstract]2026 Jun 22. PMID: 42382697 -
Theranostics
Engaging natural regulatory myeloid cells to restrict T-cell hyperactivation-induced liver inflammation via extracellular vesicle-mediated purine metabolism regulation. [Abstract]2024 Aug 12;14(12):4874-4893. PMID: 39239508 -
Theranostics
Histone deacetylase inhibitors promote epithelial-mesenchymal transition in Hepatocellular Carcinoma via AMPK-FOXO1-ULK1 signaling axis-mediated autophagy. [Abstract]2020 Aug 13;10(22):10245-10261. PMID: 32929346 -
MedComm (2020)
Novel antibiotics against Staphylococcus aureus without detectable resistance by targeting proton motive force and FtsH. [Abstract]2025 Jan 8;6(1):e70046. PMID: 39781293 -
J Exp Med
2026 Jan 5;223(1):e20250440. PMID: 41165618 -
Cell Chem Biol
The cytotoxic effect of prexasertib is a consequence of dual inhibition on both CHK1 and AMPK. [Abstract]2026 Jun 18;33(6):767-784.e7. PMID: 42061410 -
Stem Cell Res Ther
CD73-expressing endometrial regenerative cell-derived exosomes mitigate acute cardiac allograft rejection through regulating adenosine metabolism in mice. [Abstract]2025 Jun 2;16(1):277. PMID: 40457389
Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Jun 2;16(1):277. [Abstract]
Flow cytometry analysis of adenosine A2aR and A2bR expression treated with Adenosine monophosphate (AMP) (100 μM).
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Stem Cell Res Ther
Exosomes derived from a mesenchymal-like endometrial regenerative cells ameliorate renal ischemia reperfusion injury through delivery of CD73. [Abstract]2025 Mar 26;16(1):148. PMID: 40140882 -
Stem Cell Res Ther
CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells. [Abstract]2023 Sep 29;14(1):277. PMID: 37775797
Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]
IFN-γ production was detected by ELISA in the cell culture supernatant, ERCs or NC-ERCs significantly inhibited the differentiation of activated CD4+ T cells to Th1 cells compared with CD73-KO-ERCs, as well as the production of IFN-γ, especially in the presence of Adenosine monophosphate (AMP) (50 μM, 72 h).
Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]
Adenosine monophosphate (AMP) (50 μM, 72 h) was introduced to the culture medium to ensure sufficient substrate for CD73 in all situations, the reduction of CD69 expression on CD4+ T cells was more apparent when cultured with NC-ERCs than CD73-KO-ERCs.
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Anal Chem
Ultrahigh Flow Regulated Discharge Coupling with Targeted Mass Spectrometry Analysis: Real Time Capturing Biomolecules in Exhaled Breath. [Abstract]2025 May 13;97(18):9827-9835. PMID: 40302637 -
Biochem Pharmacol
Exosomal CD73 from endometrial regenerative cells confers protective effects against ischemia-reperfusion injury in kidney transplantation. [Abstract]2026 Sep;251(Pt 1):118080. PMID: 42162765 -
Molecules
Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]2025 Mar 9;30(6):1224. PMID: 40142001 -
Molecules
Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]2023 Apr 11;28(8):3375. PMID: 37110608 -
Mar Pollut Bull
Microplastics exposure affects the feeding behavior of the whiteleg shrimp Litopenaeus vannamei by impairing the appetite, olfaction and digestion. [Abstract]2025 Nov:220:118455. PMID: 40680651 -
Genes (Basel)
2022 Dec 16;13(12):2384. PMID: 36553651
Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Genes (Basel). 2022 Dec 16;13(12):2384. [Abstract]
The Survival rate of M. coruscus larvae after being treated with Adenosine monophosphate (AMP) (0.5, 1. 2.5 mM, 72 h) The figures in the legend represent the concentration (mM).
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Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Biochem Biophys Res Commun
Feedback activation of CD73-Adenosine axis attenuates the antitumor immunity of STING pathway. [Abstract]2024 May 14:708:149814. PMID: 38531218
Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]
The levels of ROS was significantly improved in the AMPD2 overexpression group after treatment with Adenosine monophosphate (AMP) (10 μM, 24 h).
Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]
Lower expression of total p38, phosphorylated p38 and Gpx4 could be observed after Adenosine monophosphate (AMP) (10 μM, 24 h) treatment in the AMPD2 overexpression group detected by western blot analysis.
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Res Sq
A phosphorylation switch at MRE11 links ATM-ATR and calcium signaling to safeguard stalled replication fork stability. [Abstract]2026 Apr 22:rs.3.rs-9192680. PMID: 42078910 -
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溶剤 & 溶解度
H2O : 5 mg/mL (13.69 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 5.26 mg/mL (14.40 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 50 mg/mL (136.90 mM); Suspended solution; Need ultrasonic
プロトコル
RAW264.7 cells are plated in 100-mm dishes and stimulated with lipopolysaccharide (LPS) for 1 h. Cross-linked chromatin are sonicated into DNA fragment of 0.5 to 1.0 kb. For in vitro biochemical experiments, the sonicated cell lysates are incubated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) at 37°C for 30 min. Then chromatin is precleared with 50% salmon sperm DNA-saturated protein A agarose beads and immunoprecipitated with 5 μg of antibody[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
The RAW264.7 cells (a murine macrophage cell line) are maintained in supplemented RPMI 1640 in an atmosphere at 90% humidity containing 5% CO2 at 37°C. At the end of the pre-incubated period, cells are rinsed with PBS and the medium is supplemented with RPMI 1640 without fetal bovine serum. The cells are pretreated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) for 30 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Male WT C57BL/6J mice are used at 8 to 10 weeks of age with a body weight of 20 to 25 g in this work. For Adenosine 5'-monophosphate monohydrate (5′-AMP)-pretreated survival experiment, WT mice are randomly divided into two groups (n=15 in each group). Adenosine 5'-monophosphate monohydrate (5 mg/20 g body weight i.p.) or PBS is administered to mice. For general experiment, WT mice are randomly divided into different groups (n=5 in each group): (1) vehicle/vehicle group; (2) Adenosine 5'-monophosphate monohydrate/vehicle group. Mice are killed and the blood is collected from the carotid artery. The liver of each mouse is removed immediately and then is kept at -80°C until analyzed[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
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参考文献
[1]. Rittiner JE, et al. AMP is an adenosine A1 receptor agonist. J Biol Chem. 2012 Feb 17;287(8):5301-9. [Content Brief]
[2]. Zhan Y, et al. Adenosine 5'-monophosphate ameliorates D-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice. Cell Death Dis. 2014 Jan 9;5:e985. [Content Brief]
[3]. Ayisi NK, et al. Comparison of the antiviral effects of 5-methoxymethyldeoxyuridine-5'-monophosphate with adenine arabinoside-5'-monophosphate. Antiviral Res. 1983 Sep;3(3):161-74. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.7379 mL | 13.6896 mL | 27.3793 mL | 68.4481 mL |
| 5 mM | 0.5476 mL | 2.7379 mL | 5.4759 mL | 13.6896 mL | |
| 10 mM | 0.2738 mL | 1.3690 mL | 2.7379 mL | 6.8448 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Adenosine 5'-monophosphate monohydrate 関連分類
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