2. Metabolic Enzyme/Protease GPCR/G Protein Anti-infection
  3. Endogenous Metabolite Adenosine Receptor HSV
  4. Adenosine 5'-monophosphate monohydrate

Adenosine 5'-monophosphate monohydrate  (Synonyms: 5'-AMP monohydrate)

Cat. No.: HY-A0181A Purity: 99.81%
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Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist. Adenosine 5'-monophosphate monohydrate has significant antiviral activity against HSV-1 and HSV-2.

For research use only. We do not sell to patients.

CAS No. : 18422-05-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Solution
10 mM * 1 mL in Water In-stock
Solid
500 mg In-stock
1 g In-stock
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Adenosine 5'-monophosphate monohydrate Related Antibodies

Top Publications Citing Use of Products

    Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Jun 2;16(1):277.  [Abstract]

    Flow cytometry analysis of adenosine A2aR and A2bR expression treated with Adenosine monophosphate (AMP) (100 μM).

    Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814.

    The levels of ROS was significantly improved in the AMPD2 overexpression group after treatment with Adenosine monophosphate (AMP) (10 μM, 24 h).

    Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814.

    Lower expression of total p38, phosphorylated p38 and Gpx4 could be observed after Adenosine monophosphate (AMP) (10 μM, 24 h) treatment in the AMPD2 overexpression group detected by western blot analysis.

    Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277.  [Abstract]

    IFN-γ production was detected by ELISA in the cell culture supernatant, ERCs or NC-ERCs significantly inhibited the differentiation of activated CD4+ T cells to Th1 cells compared with CD73-KO-ERCs, as well as the production of IFN-γ, especially in the presence of Adenosine monophosphate (AMP) (50 μM, 72 h).

    Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277.  [Abstract]

    Adenosine monophosphate (AMP) (50 μM, 72 h) was introduced to the culture medium to ensure sufficient substrate for CD73 in all situations, the reduction of CD69 expression on CD4+ T cells was more apparent when cultured with NC-ERCs than CD73-KO-ERCs.

    Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Genes (Basel). 2022 Dec 16;13(12):2384.  [Abstract]

    The Survival rate of M. coruscus larvae after being treated with Adenosine monophosphate (AMP) (0.5, 1. 2.5 mM, 72 h) The figures in the legend represent the concentration (mM).

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist. Adenosine 5'-monophosphate monohydrate has significant antiviral activity against HSV-1 and HSV-2[1][2][3].

    IC50 & Target

    Microbial Metabolite

     

    HSV-1

     

    HSV-2

     

    Human Endogenous Metabolite

     

    In Vitro

    Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist[1]. Adenosine 5'-monophosphate monohydrate (5′-AMP)-induced cell toxicity is negligible at concentrations of 25 to 400 μM in RAW264.7 cells. Adenosine 5'-monophosphate monohydrate significantly attenuates the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in RAW264.7 cells. The dose-dependence of TNF-α and IL-6 mRNA show that at concentration of 400 μM, Adenosine 5'-monophosphate monohydrate exhibits the maximum inhibition. Exposure of cells to Adenosine 5'-monophosphate monohydrate significantly reduces recruitment of NF-κB p65 to TNF-α, IL-6, and IL-1β gene promoters[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Adenosine 5'-monophosphate monohydrate Related Antibodies
    In Vivo

    C57BL/6J mice treated with Adenosine 5'-monophosphate monohydrate (5′-AMP) markedly increases hepatic adenosine level. Survival rate in PBS-treated mice (n=15) is 60% (8 h) and 33.3% (24 h), whereas survival rate in Adenosine 5'-monophosphate monohydrate-treated mice (n=15) is 100% (8 h) and 93.3% (24 h). Serum aspartate transaminase (AST) and alanine transaminase (ALT) levels are significantly lowered in the Adenosine 5'-monophosphate monohydrate group than in the vehicle group. The area and extent of necrosis is attenuated and the infiltration of inflammatory cells is reduced in the Adenosine 5'-monophosphate monohydrate group[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    365.24

    Formula

    C10H16N5O8P
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C=NC3=C2N=CN=C3N.O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    H2O : 5 mg/mL (13.69 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7379 mL 13.6896 mL 27.3793 mL
    5 mM 0.5476 mL 2.7379 mL 5.4759 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    C2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 5.26 mg/mL (14.40 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    • Protocol 2

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 50 mg/mL (136.90 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.81%

    SDS (254 KB)

    COA (254 KB) HNMR (134 KB) RP-HPLC (86 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [2]

    RAW264.7 cells are plated in 100-mm dishes and stimulated with lipopolysaccharide (LPS) for 1 h. Cross-linked chromatin are sonicated into DNA fragment of 0.5 to 1.0 kb. For in vitro biochemical experiments, the sonicated cell lysates are incubated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) at 37°C for 30 min. Then chromatin is precleared with 50% salmon sperm DNA-saturated protein A agarose beads and immunoprecipitated with 5 μg of antibody[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [2]

    The RAW264.7 cells (a murine macrophage cell line) are maintained in supplemented RPMI 1640 in an atmosphere at 90% humidity containing 5% CO2 at 37°C. At the end of the pre-incubated period, cells are rinsed with PBS and the medium is supplemented with RPMI 1640 without fetal bovine serum. The cells are pretreated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) for 30 min[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Male WT C57BL/6J mice are used at 8 to 10 weeks of age with a body weight of 20 to 25 g in this work. For Adenosine 5'-monophosphate monohydrate (5′-AMP)-pretreated survival experiment, WT mice are randomly divided into two groups (n=15 in each group). Adenosine 5'-monophosphate monohydrate (5 mg/20 g body weight i.p.) or PBS is administered to mice. For general experiment, WT mice are randomly divided into different groups (n=5 in each group): (1) vehicle/vehicle group; (2) Adenosine 5'-monophosphate monohydrate/vehicle group. Mice are killed and the blood is collected from the carotid artery. The liver of each mouse is removed immediately and then is kept at -80°C until analyzed[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.7379 mL 13.6896 mL 27.3793 mL 68.4481 mL
    5 mM 0.5476 mL 2.7379 mL 5.4759 mL 13.6896 mL
    10 mM 0.2738 mL 1.3690 mL 2.7379 mL 6.8448 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Adenosine 5'-monophosphate monohydrate Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Adenosine 5'-monophosphate18422-05-45'-AMPEndogenous MetaboliteAdenosine ReceptorHSVP1 receptorHerpes simplex virusInhibitorinhibitorinhibit

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