Cat. No.: HY-A0181A

Adenosine 5'-monophosphate monohydrate

(Synonyms: 5'-AMP monohydrate)

22 Cited Publications

Customer Review

Based on 22 publication(s) in Google Scholar

Adenosine 5'-monophosphate monohydrate is an adenosine A₁ receptor agonist. Adenosine 5'-monophosphate monohydrate has significant antiviral activity against HSV-1 and HSV-2.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 18422-05-4
Formula: C₁₀H₁₆N₅O₈P
Molecular Weight:365.24

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Adenosine 5'-monophosphate monohydrate Chemical Structure

CAS No.: 18422-05-4

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (22)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Adenosine 5'-monophosphate monohydrate is an adenosine A₁ receptor agonist. Adenosine 5'-monophosphate monohydrate has significant antiviral activity against HSV-1 and HSV-2^[1]^[2]^[3].

IC₅₀ & Target

Microbial Metabolite

HSV-1

HSV-2

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All Adenosine Receptor Isoforms :

All HSV Isoforms :

In Vitro

Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist^[1]. Adenosine 5'-monophosphate monohydrate (5′-AMP)-induced cell toxicity is negligible at concentrations of 25 to 400 μM in RAW264.7 cells. Adenosine 5'-monophosphate monohydrate significantly attenuates the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in RAW264.7 cells. The dose-dependence of TNF-α and IL-6 mRNA show that at concentration of 400 μM, Adenosine 5'-monophosphate monohydrate exhibits the maximum inhibition. Exposure of cells to Adenosine 5'-monophosphate monohydrate significantly reduces recruitment of NF-κB p65 to TNF-α, IL-6, and IL-1β gene promoters^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

C57BL/6J mice treated with Adenosine 5'-monophosphate monohydrate (5′-AMP) markedly increases hepatic adenosine level. Survival rate in PBS-treated mice (n=15) is 60% (8 h) and 33.3% (24 h), whereas survival rate in Adenosine 5'-monophosphate monohydrate-treated mice (n=15) is 100% (8 h) and 93.3% (24 h). Serum aspartate transaminase (AST) and alanine transaminase (ALT) levels are significantly lowered in the Adenosine 5'-monophosphate monohydrate group than in the vehicle group. The area and extent of necrosis is attenuated and the infiltration of inflammatory cells is reduced in the Adenosine 5'-monophosphate monohydrate group^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 18422-05-4
Appearance Solid
Molecular Weight 365.24
Formula C₁₀H₁₆N₅O₈P
Color White to off-white
SMILES

O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C=NC3=C2N=CN=C3N.O
Synonyms

5'-AMP monohydrate
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (22)

All Flow CytometryELISACell Proliferation/Viability AssayCell Imaging/StainingWB

Sort by:

Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Cell

Adenosine kinase and ADAL coordinate detoxification of modified adenosines to safeguard metabolism. [Abstract]

2025 Oct 30;188(22):6151-6169.e24. PMID: 40840445
Exploration

Radiolabeled Coordination Polymer-Loaded Microneedles for Synergistic Melanoma Brachytherapy-Immunotherapy via STING Activation and Pyroptosis. [Abstract]

2026 Jun 22. PMID: 42382697
Theranostics

Engaging natural regulatory myeloid cells to restrict T-cell hyperactivation-induced liver inflammation via extracellular vesicle-mediated purine metabolism regulation. [Abstract]

2024 Aug 12;14(12):4874-4893. PMID: 39239508
Theranostics

Histone deacetylase inhibitors promote epithelial-mesenchymal transition in Hepatocellular Carcinoma via AMPK-FOXO1-ULK1 signaling axis-mediated autophagy. [Abstract]

2020 Aug 13;10(22):10245-10261. PMID: 32929346
MedComm (2020)

Novel antibiotics against Staphylococcus aureus without detectable resistance by targeting proton motive force and FtsH. [Abstract]

2025 Jan 8;6(1):e70046. PMID: 39781293
J Exp Med

Xbp1 controls the reparative function of intestinal ILC2s during colitis. [Abstract]

2026 Jan 5;223(1):e20250440. PMID: 41165618
Cell Chem Biol

The cytotoxic effect of prexasertib is a consequence of dual inhibition on both CHK1 and AMPK. [Abstract]

2026 Jun 18;33(6):767-784.e7. PMID: 42061410
Stem Cell Res Ther

CD73-expressing endometrial regenerative cell-derived exosomes mitigate acute cardiac allograft rejection through regulating adenosine metabolism in mice. [Abstract]

2025 Jun 2;16(1):277. PMID: 40457389

Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Jun 2;16(1):277. [Abstract]

Flow cytometry analysis of adenosine A2aR and A2bR expression treated with Adenosine monophosphate (AMP) (100 μM).
Stem Cell Res Ther

Exosomes derived from a mesenchymal-like endometrial regenerative cells ameliorate renal ischemia reperfusion injury through delivery of CD73. [Abstract]

2025 Mar 26;16(1):148. PMID: 40140882
Stem Cell Res Ther

CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells. [Abstract]

2023 Sep 29;14(1):277. PMID: 37775797

Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]

IFN-γ production was detected by ELISA in the cell culture supernatant, ERCs or NC-ERCs significantly inhibited the differentiation of activated CD4⁺ T cells to Th1 cells compared with CD73-KO-ERCs, as well as the production of IFN-γ, especially in the presence of Adenosine monophosphate (AMP) (50 μM, 72 h).

Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]

Adenosine monophosphate (AMP) (50 μM, 72 h) was introduced to the culture medium to ensure sufficient substrate for CD73 in all situations, the reduction of CD69 expression on CD4⁺ T cells was more apparent when cultured with NC-ERCs than CD73-KO-ERCs.
Anal Chem

Ultrahigh Flow Regulated Discharge Coupling with Targeted Mass Spectrometry Analysis: Real Time Capturing Biomolecules in Exhaled Breath. [Abstract]

2025 May 13;97(18):9827-9835. PMID: 40302637
Biochem Pharmacol

Exosomal CD73 from endometrial regenerative cells confers protective effects against ischemia-reperfusion injury in kidney transplantation. [Abstract]

2026 Sep;251(Pt 1):118080. PMID: 42162765
Molecules

Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]

2025 Mar 9;30(6):1224. PMID: 40142001
Molecules

Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]

2023 Apr 11;28(8):3375. PMID: 37110608
Mar Pollut Bull

Microplastics exposure affects the feeding behavior of the whiteleg shrimp Litopenaeus vannamei by impairing the appetite, olfaction and digestion. [Abstract]

2025 Nov:220:118455. PMID: 40680651
Genes (Basel)

AMPK Promotes Larval Metamorphosis of Mytilus coruscus. [Abstract]

2022 Dec 16;13(12):2384. PMID: 36553651

Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Genes (Basel). 2022 Dec 16;13(12):2384. [Abstract]

The Survival rate of M. coruscus larvae after being treated with Adenosine monophosphate (AMP) (0.5, 1. 2.5 mM, 72 h) The figures in the legend represent the concentration (mM).
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biochem Biophys Res Commun

Feedback activation of CD73-Adenosine axis attenuates the antitumor immunity of STING pathway. [Abstract]

2024 May 14:708:149814. PMID: 38531218

Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]

The levels of ROS was significantly improved in the AMPD2 overexpression group after treatment with Adenosine monophosphate (AMP) (10 μM, 24 h).

Adenosine 5'-monophosphate monohydrate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]

Lower expression of total p38, phosphorylated p38 and Gpx4 could be observed after Adenosine monophosphate (AMP) (10 μM, 24 h) treatment in the AMPD2 overexpression group detected by western blot analysis.
Res Sq

A phosphorylation switch at MRE11 links ATM-ATR and calcium signaling to safeguard stalled replication fork stability. [Abstract]

2026 Apr 22:rs.3.rs-9192680. PMID: 42078910
University of Cambridge

2023 Feb.
Research Square Preprint

2022 May.

Solvent & Solubility

In Vitro:

H₂O : 5 mg/mL (13.69 mM; Need ultrasonic and warming)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7379 mL

13.6896 mL

27.3793 mL

5 mM

0.5476 mL

2.7379 mL

5.4759 mL

10 mM

0.2738 mL

1.3690 mL

2.7379 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 5.26 mg/mL (14.40 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

Protocol 2

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 50 mg/mL (136.90 mM); Suspended solution; Need ultrasonic

Protocol

Kinase Assay^[2]

RAW264.7 cells are plated in 100-mm dishes and stimulated with lipopolysaccharide (LPS) for 1 h. Cross-linked chromatin are sonicated into DNA fragment of 0.5 to 1.0 kb. For in vitro biochemical experiments, the sonicated cell lysates are incubated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) at 37°C for 30 min. Then chromatin is precleared with 50% salmon sperm DNA-saturated protein A agarose beads and immunoprecipitated with 5 μg of antibody^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay^[2]

The RAW264.7 cells (a murine macrophage cell line) are maintained in supplemented RPMI 1640 in an atmosphere at 90% humidity containing 5% CO₂ at 37°C. At the end of the pre-incubated period, cells are rinsed with PBS and the medium is supplemented with RPMI 1640 without fetal bovine serum. The cells are pretreated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) for 30 min^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Male WT C57BL/6J mice are used at 8 to 10 weeks of age with a body weight of 20 to 25 g in this work. For Adenosine 5'-monophosphate monohydrate (5′-AMP)-pretreated survival experiment, WT mice are randomly divided into two groups (n=15 in each group). Adenosine 5'-monophosphate monohydrate (5 mg/20 g body weight i.p.) or PBS is administered to mice. For general experiment, WT mice are randomly divided into different groups (n=5 in each group): (1) vehicle/vehicle group; (2) Adenosine 5'-monophosphate monohydrate/vehicle group. Mice are killed and the blood is collected from the carotid artery. The liver of each mouse is removed immediately and then is kept at -80°C until analyzed^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Rittiner JE, et al. AMP is an adenosine A1 receptor agonist. J Biol Chem. 2012 Feb 17;287(8):5301-9. [Content Brief]

[2]. Zhan Y, et al. Adenosine 5'-monophosphate ameliorates D-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice. Cell Death Dis. 2014 Jan 9;5:e985. [Content Brief]

[3]. Ayisi NK, et al. Comparison of the antiviral effects of 5-methoxymethyldeoxyuridine-5'-monophosphate with adenine arabinoside-5'-monophosphate. Antiviral Res. 1983 Sep;3(3):161-74. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.7379 mL	13.6896 mL	27.3793 mL	68.4481 mL
	5 mM	0.5476 mL	2.7379 mL	5.4759 mL	13.6896 mL
	10 mM	0.2738 mL	1.3690 mL	2.7379 mL	6.8448 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Adenosine 5'-monophosphate monohydrate

22 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Adenosine 5'-monophosphate monohydrate

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Infection Compound Library

GPCR/G Protein Compound Library

Metabolism/Protease Compound Library

Natural Product Library

FDA-Approved Drug Library

Antiviral Compound Library

Human Endogenous Metabolite Compound Library

Small Molecule Immuno-Oncology Compound Library

Drug Repurposing Compound Library

Anti-COVID-19 Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Neurotransmitter Receptor Compound Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Neuroprotective Compound Library

Microbial Metabolite Library

Food-Sourced Compound Library

Antiviral Traditional Chinese Medicine Active Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Off-patent Drug Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Animal-Sourced Natural Product Library

FDA-Approved Traditional Chinese Medicine Active Compound Library

Multi-Target Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Natural Product and Natural Product-Like Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Mouse Metabolite Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Nucleotide Metabolite Compound Library

Flavors and Fragrances Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Adenosine 5'-monophosphate monohydrate

All Endogenous Metabolite Isoforms

All Adenosine Receptor Isoforms

All HSV Isoforms

Biological Activity

Chemical Information

Publications (22)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Adenosine 5'-monophosphate monohydrate Related Classifications

HY-A0181A Related Classifications