Gentamicin sulfate
Based on 64 publication(s) in Google Scholar
Gentamicin sulfate, an aminoglycoside antibiotic, inhibits the growth of both gram-positive and gram-negative bacteria and to inhibit several strains of mycoplasma in tissue culture. Gentamicin sulfate inhibits DNase I with an IC50 of 0.57 mM.
For research use only. We do not sell to patients.
- CAS No.: 1405-41-0
- Formula: C24H55N7O11S3*
- Molecular Weight:561.65 (Average)
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Gentamicin sulfate
More- Cancer Cell. 2025 Jul 29:S1535-6108(25)00318-6. [Abstract]
- Cell. 2024 Feb 15;187(4):882-896.e17 [Abstract]
- Nat Aging. 2025 May;5(5):848-867. [Abstract]
- Nat Microbiol. 2023 Mar;8(3):410-423. [Abstract]
- Cell Host Microbe. 2024 Jul 23:S1931-3128(24)00254-3. [Abstract]
- Cancer Res. 2025 Oct 9. [Abstract]
- Nat Commun. 2022 Mar 2;13(1):1116. [Abstract]
- Autophagy. 2025 Jul 27:1-23. [Abstract]
- MedComm (2020). 2025 Jan 8;6(1):e70046. [Abstract]
- J Exp Med. 2026 Mar 2;223(3):e20241287. [Abstract]
- Int J Biol Sci. 2024 Oct 7;20(14):5415-5435. [Abstract]
- Food Chem. 2023 Mar 1:403:134399. [Abstract]
- Cell Death Dis. 2023 Jan 11;14(1):15. [Abstract]
- Int J Biol Macromol. 2025 May;305(Pt 2):141278. [Abstract]
- ACS Appl Mater Interfaces. 2019 Oct 16;11(41):38190-38204. [Abstract]
- Emerg Microbes Infect. 2024 Dec;13(1):2321981. [Abstract]
- Biomed Pharmacother. 2023 Dec 31:169:115856. [Abstract]
- Curr Res Food Sci. 2025 May 16:10:101084. [Abstract]
- Biochem Pharmacol. 2025 Dec;242(Pt 2):117343. [Abstract]
- World J Gastroenterol. 2025 Jul 28;31(28):107361. [Abstract]
- Food Funct. 2025 Feb 3;16(3):1041-1059. [Abstract]
- Virulence. 2024 Dec;15(1):2415952. [Abstract]
- J Ethnopharmacol. 2020 Sep 15;259:112882. [Abstract]
- Mikrochim Acta. 2025 Dec 27;193(1):49. [Abstract]
- Int Immunopharmacol. 2026 Apr 15:175:116472. [Abstract]
- Int Immunopharmacol. 2025 May 21:159:114888. [Abstract]
- Am J Physiol Cell Physiol. 2019 Aug 1;317(2):C277-C286. [Abstract]
- Toxicology. 2023 Jan 1:483:153386. [Abstract]
- Int J Infect Dis. 2026 Jan:162:108165. [Abstract]
- Appl Microbiol Biotechnol. 2022 Apr;106(7):2689-2702. [Abstract]
- FASEB J. 2026 Jan 31;40(2):e71438. [Abstract]
- BMC Microbiol. 2025 Apr 29;25(1):257. [Abstract]
- Microorganisms. 2025 Apr 18;13(4):938. [Abstract]
- Microorganisms. 2024 Mar 13;12(3):575. [Abstract]
- Aquaculture. 2021, 736248.
- Microbiol Spectr. 2025 Oct 16:e0160325. [Abstract]
- Microbiol Spectr. 2025 Aug 29:e0059025. [Abstract]
- Microbiol Spectr. 2025 May 19:e0307424. [Abstract]
- ACS Infect Dis. 2024 Apr 12;10(4):1327-1338. [Abstract]
- AMB Express. 2024 Dec 24;14(1):141. [Abstract]
- Transl Lung Cancer Res. 2025 Jun 30;14(6):2159-2179. [Abstract]
- Mol Med Rep. 2016 Dec;14(6):5304-5310. [Abstract]
- Pathogens. 2024 Nov 15;13(11):1003. [Abstract]
- Cytotherapy. 2025 Dec;27(12):1419-1426. [Abstract]
- Cell Transplant. 2021 Jan-Dec:30:963689721997151. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2025 Apr;398(4):3681-3695. [Abstract]
- Mol Immunol. 2017 Dec:92:151-160. [Abstract]
- Exp Eye Res. 2025 Oct 16:262:110698. [Abstract]
- Immun Inflamm Dis. 2024 Jul;12(7):e1149. [Abstract]
- Vet Microbiol. 2024 May:292:110046. [Abstract]
- Curr Microbiol. 2025 Aug 16;82(10):456. [Abstract]
- Infect Genet Evol. 2025 Sep:133:105780. [Abstract]
- Curr Microbiol. 2023 May 31;80(7):230. [Abstract]
- Mutat Res Genet Toxicol Environ Mutagen. 2023 May-Jun:888:503636. [Abstract]
- Future Microbiol. 2025 Oct 13:1-10. [Abstract]
- Physiol Res. 2025 Mar 24;74(1):79-92. [Abstract]
- Vet Res Commun. 2024 Dec;48(6):3859-3872. [Abstract]
- Int J Med Microbiol. 2023 Mar 28;313(2):151578. [Abstract]
- Chin J Traumatol. 2024 Jul 3:S1008-1275(24)00075-0. [Abstract]
- Bio Protoc. 2025 Feb 20;15(4):e5221. [Abstract]
- Patent. US20240319170A1
- bioRxiv. 2024 May 10.
- Research Square Print. October 6th, 2022.
- Evid Based Complement Alternat Med. 2015:2015:639412. [Abstract]
Customer Validation & Images
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Microbiological Assay
All Antibiotic Isoforms
More
Biological Activity
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Aminoglycoside |
Gentamicin sulfate is a more effective in vitro bacterial inhibitor than combined penicillin-streptomycin, is nontoxic to tissue culture monolayers, and does not inhibit virus replication[2].
Gentamicin sulfate has been used with success as an additive in commercial mycology media to inhibit growth of bacteria and has been shown to be bactericidal for a wider range of organisms (Pseudomonas aeruginosa, Proteus sp., and Streptococcus faecalis) than penicillin and streptomycin[2].
Gentamicin sulfate does not interfere with the production of cytopathic effect by certain echoviruses and polioviruses in tissue culture, is nontoxic to Rhesus monkey kidney, HeLa, and human amnion cells, and is stable at autoclave temperatures[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:FVB/N mice (6-10-week-old; ~20 g) received an intratracheal injection of a solution containing the ATCC 27853 strain of P. aeruginosa[3].
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Dosage:10 mg/kg
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Administration:Subcutaneous injection (s.c.); every 12 h for a total of six doses
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Result:Survival at 7 days was 100% for mice. Down-regulated the proinflammatory cytokines, IL-6.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1405-41-0
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Appearance Solid
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Molecular Weight 561.65 (Average)
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Formula C24H55N7O11S3*
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Color White to off-white
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SMILES
O=S(O)(O)=O.O[C@]1(C)C(NC)[C@@H](O)[C@@H](O[C@H]2C(N)C[C@H](N)[C@@H](O[C@@H]3[C@H](N)CC[C@@H]([R])O3)[C@@H]2O)OC1.NC[*].C[C@H]([*])N.C[C@H]([*])NC.[R=].[or].[or]
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Initial Source
Micromonospora purpurea
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (64)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Tumor-resident probiotic Clostridium butyricum improves aPD-1 efficacy in colorectal cancer models by inhibiting IL-6-mediated immunosuppression. [Abstract]2025 Jul 29:S1535-6108(25)00318-6. PMID: 40780216 -
Cell
Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice. [Abstract]2024 Feb 15;187(4):882-896.e17 PMID: 38295787 -
Nat Aging
mRNA metabolism regulator human antigen R (HuR) regulates age-related hearing loss in aged mice. [Abstract]2025 May;5(5):848-867. PMID: 40394214 -
Nat Microbiol
Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strains. [Abstract]2023 Mar;8(3):410-423. PMID: 36759752 -
Cell Host Microbe
Peptostreptococcus stomatis promotes colonic tumorigenesis and receptor tyrosine kinase inhibitor resistance by activating ERBB2-MAPK. [Abstract]2024 Jul 23:S1931-3128(24)00254-3. PMID: 39059397 -
Cancer Res
2025 Oct 9. PMID: 41066593 -
Nat Commun
Antimicrobial resistance and population genomics of multidrug-resistant Escherichia coli in pig farms in mainland China. [Abstract]2022 Mar 2;13(1):1116. PMID: 35236849 -
Autophagy
Restricting intracellular Salmonella proliferation by coordinating p-TBK1 mediated mitophagy and xenophagy. [Abstract]2025 Jul 27:1-23. PMID: 40660474 -
MedComm (2020)
Novel antibiotics against Staphylococcus aureus without detectable resistance by targeting proton motive force and FtsH. [Abstract]2025 Jan 8;6(1):e70046. PMID: 39781293 -
J Exp Med
2026 Mar 2;223(3):e20241287. PMID: 41400657 -
Int J Biol Sci
Heterophyllin B enhances transcription factor EB-mediated autophagy and alleviates pyroptosis and oxidative stress after spinal cord injury. [Abstract]2024 Oct 7;20(14):5415-5435. PMID: 39494322 -
Food Chem
Electrochemical sensor for detecting streptomycin in milk based on label-free aptamer chain and magnetic adsorption. [Abstract]2023 Mar 1:403:134399. PMID: 36182859 -
Cell Death Dis
Use of mouse primary epidermal organoids for USA300 infection modeling and drug screening. [Abstract]2023 Jan 11;14(1):15. PMID: 36631452 -
Int J Biol Macromol
Bacteria escape macrophage-mediated phagocytosis via targeting apurinic/apyrimidinic endonuclease 1 in sepsis. [Abstract]2025 May;305(Pt 2):141278. PMID: 39984093 -
ACS Appl Mater Interfaces
Effect of PEGylated Magnetic PLGA-PEI Nanoparticles on Primary Hippocampal Neurons: Reduced Nanoneurotoxicity and Enhanced Transfection Efficiency with Magnetofection. [Abstract]2019 Oct 16;11(41):38190-38204. PMID: 31550131 -
Emerg Microbes Infect
AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. [Abstract]2024 Dec;13(1):2321981. PMID: 38422452 -
Biomed Pharmacother
The enhancement effect of small molecule Lyb24 reveals AzoR as a novel target of polymyxin B. [Abstract]2023 Dec 31:169:115856. PMID: 37949698 -
Curr Res Food Sci
Gallic acid prevents obesity in mice on a high-fat diet via the gut microbiota-adipose tissue axis. [Abstract]2025 May 16:10:101084. PMID: 40491530 -
Biochem Pharmacol
Diversity of human choline transporters: substrate specificity, kinetic properties, and inhibitor sensitivity. [Abstract]2025 Dec;242(Pt 2):117343. PMID: 40972734 -
World J Gastroenterol
FBP1 as a key regulator of focal adhesion kinase-mediated hepatic stellate cell activation: Multi-omics and experimental validation. [Abstract]2025 Jul 28;31(28):107361. PMID: 40741470 -
Food Funct
Dietary limonin alleviates Salmonella Typhimurium-induced colitis via dual targeting virulence SopB and SopE2 and inhibiting RAC1/CDC42/Arp2/3 pathway and regulating gut microbiota. [Abstract]2025 Feb 3;16(3):1041-1059. PMID: 39820212 -
Virulence
Bactericidal and anti-quorum sensing activity of repurposing drug Visomitin against Staphylococcus aureus. [Abstract]2024 Dec;15(1):2415952. PMID: 39390774 -
J Ethnopharmacol
The water extract of "Jiao Mei Gu" attenuates the lipopolysaccharide-induced inflammatory response via inhibiting NF-κB activity in mice. [Abstract]2020 Sep 15;259:112882. PMID: 32325181 -
Mikrochim Acta
Highly sensitive and accurate detection of dam MTase activity via G-switch-activated AgNCs fluorescence. [Abstract]2025 Dec 27;193(1):49. PMID: 41454998 -
Int Immunopharmacol
Artesunate ameliorates Staphylococcus aureus-induced mandibular osteomyelitis and promotes osteogenic differentiation via NOD2/p65 signaling pathway. [Abstract]2026 Apr 15:175:116472. PMID: 41791305 -
Int Immunopharmacol
Early-life antibiotic dysbiosis impairs microbial tryptophan- nicotinic acid metabolism exacerbating food allergy in adulthood. [Abstract]2025 May 21:159:114888. PMID: 40403504 -
Am J Physiol Cell Physiol
Electrical stimulation facilitates the angiogenesis of human umbilical vein endothelial cells through MAPK/ERK signaling pathway by stimulating FGF2 secretion. [Abstract]2019 Aug 1;317(2):C277-C286. PMID: 30995109 -
Toxicology
Embryotoxicity evaluation of Gentamicin, an aminoglycoside antibiotic added to human embryo culture medium, using the zebrafish (Danio rerio) model. [Abstract]2023 Jan 1:483:153386. PMID: 36460222 -
Int J Infect Dis
In vitro characterization of delafloxacin activity against intracellular Legionella: Exploring new treatment options for Legionnaires' disease. [Abstract]2026 Jan:162:108165. PMID: 41187873 -
Appl Microbiol Biotechnol
2022 Apr;106(7):2689-2702. PMID: 35338386 -
FASEB J
Enhancing the Susceptibility of Methicillin-Resistant Staphylococcus aureus to β-Lactam Antibiotics Through the Use of 4-Methoxysalicyl Aldehyde. [Abstract]2026 Jan 31;40(2):e71438. PMID: 41524623 -
BMC Microbiol
Klebsiella pneumoniae under xylose pressure: the growth adaptation, antimicrobial susceptibility, global proteomics analysis and role of XylA and XylB proteins. [Abstract]2025 Apr 29;25(1):257. PMID: 40301709 -
Microorganisms
Isolation, Antimicrobial Susceptibility, and Genotypes of Three Pasteurellaeae Species Prevalent on Pig Farms in China Between 2021 and 2023. [Abstract]2025 Apr 18;13(4):938. PMID: 40284774 -
Microorganisms
Antimicrobial Resistance and Genomic Characteristics of Escherichia coli Strains Isolated from the Poultry Industry in Henan Province, China. [Abstract]2024 Mar 13;12(3):575. PMID: 38543626
Gentamicin sulfate purchased from MedChemExpress. Usage Cited in: Microorganisms. 2024 Mar 13;12(3):575. [Abstract]
Percentages of E. coli strains showing antimicrobial resistance to different antimicrobial agents. AMP, Ampicillin; CAZ, Ceftazidime; IPM, Imipenem; MRP, Meropenem; GEN, Gentamicin; OFX, Ofloxacin; SXT, Trimethoprim–sulfamethoxazole; TET, Tetracycline; TGC, Tigecycline; CL, Colistin; FFC, Florfenicol.
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Microbiol Spectr
The adaptive growth and mechanisms of Klebsiella pneumoniae under sucrose and glucose exposure. [Abstract]2025 Oct 16:e0160325. PMID: 41099518 -
Microbiol Spectr
2025 Aug 29:e0059025. PMID: 40879403 -
Microbiol Spectr
Antimicrobial resistance of rapidly growing mycobacteria isolated from companion animals in Taiwan. [Abstract]2025 May 19:e0307424. PMID: 40387379 -
ACS Infect Dis
Lusutrombopag as a Repurposing Drug in Combination with Aminoglycosides against Vancomycin-Resistant Enterococcus. [Abstract]2024 Apr 12;10(4):1327-1338. PMID: 38567846 -
AMB Express
Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms. [Abstract]2024 Dec 24;14(1):141. PMID: 39718732 -
Transl Lung Cancer Res
Development of a 3D-3 co-culture microbead consisting of cancer-associated fibroblasts and human umbilical vein endothelial cells for the anti-tumor drug assessment of lung cancer. [Abstract]2025 Jun 30;14(6):2159-2179. PMID: 40673102 -
Mol Med Rep
Mitochondrial transcription factor A contributes to cisplatin resistance in patients with estrogen receptor‑positive breast cancer. [Abstract]2016 Dec;14(6):5304-5310. PMID: 27779689 -
Pathogens
Mycoplasma bovis Invades Non-Phagocytic Cells by Clathrin-Dependent Endocytic Pathways and Escapes from Phagocytic Vesicles. [Abstract]2024 Nov 15;13(11):1003. PMID: 39599557 -
Cytotherapy
NLRC5 knockdown in CD4+CD25+ Treg cells attenuates cardiac allograft rejection through enhanced immunosuppressive function. [Abstract]2025 Dec;27(12):1419-1426. PMID: 41085514 -
Cell Transplant
Protective Effects of Reduced Glutathione and Ulinastatin on Xeno-transplanted Human Ovarian Tissue Against Ischemia and Reperfusion Injury. [Abstract]2021 Jan-Dec:30:963689721997151. PMID: 33784205 -
Naunyn Schmiedebergs Arch Pharmacol
5-aminosalicylic acid alleviates colitis and protects intestinal barrier function by modulating gut microbiota in mice. [Abstract]2025 Apr;398(4):3681-3695. PMID: 39352537 -
Mol Immunol
MOTS-c peptide increases survival and decreases bacterial load in mice infected with MRSA. [Abstract]2017 Dec:92:151-160. PMID: 29096170 -
Exp Eye Res
Astragalus polysaccharide protects mouse cornea from type 1 diabetes and activates AMPK-dependent autophagy. [Abstract]2025 Oct 16:262:110698. PMID: 41109591 -
Immun Inflamm Dis
Increase in bile acids after sleeve gastrectomy improves metabolism by activating GPBAR1 to increase cAMP in mice with nonalcoholic fatty liver disease. [Abstract]2024 Jul;12(7):e1149. PMID: 39031498 -
Vet Microbiol
Discovery of the tigecycline resistance gene cluster tmexCD3-toprJ1 in Pasteurella multocida strains isolated from pigs in China. [Abstract]2024 May:292:110046. PMID: 38471428 -
Curr Microbiol
Shaking Angles Determine Staphylococcus aureus Virulence Via Oxygen-Glucose Metabolic Crosstalk in Vitro. [Abstract]2025 Aug 16;82(10):456. PMID: 40817921 -
Infect Genet Evol
Polymyxin B combined with amikacin delays the resistance of Klebsiella pneumoniae to polymyxin B by modulating the expression of NlpE. [Abstract]2025 Sep:133:105780. PMID: 40480594 -
Curr Microbiol
Repurposing CD5789 as an Antimicrobial Agent Against MRSA and Its High Resistant Phonotypes. [Abstract]2023 May 31;80(7):230. PMID: 37256372 -
Mutat Res Genet Toxicol Environ Mutagen
Helicobacter pylori plus N-Methyl-N'-nitro-N-nitrosoguanidine: DNA damage and repair: Malignant transformation of human esophageal epithelial cells. [Abstract]2023 May-Jun:888:503636. PMID: 37188438 -
Future Microbiol
2025 Oct 13:1-10. PMID: 41082411 -
Physiol Res
The Role of Mdivi-1 in Reducing Mitochondrial Fission via the NF-kappaB/JNK/SIRT3 Signaling Pathway in Acute Kidney Injury. [Abstract]2025 Mar 24;74(1):79-92. PMID: 40126145 -
Vet Res Commun
Biological characterization of lipoic acid- and heme-dependent Escherichia coli small colony variants isolated from sheep in Xinjiang, China. [Abstract]2024 Dec;48(6):3859-3872. PMID: 39325108 -
Int J Med Microbiol
Antibacterial and antibiofilm efficacy of repurposing drug hexestrol against methicillin-resistant Staphylococcus aureus. [Abstract]2023 Mar 28;313(2):151578. PMID: 37001448 -
Chin J Traumatol
The cytochrome P4501A1 (CYP1A1) inhibitor bergamottin enhances host tolerance to multidrug-resistant Vibrio vulnificus infection. [Abstract]2024 Jul 3:S1008-1275(24)00075-0. PMID: 38981821 -
Bio Protoc
2025 Feb 20;15(4):e5221. PMID: 40028013 -
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Evid Based Complement Alternat Med
Electroacupuncture at Bilateral Zusanli Points (ST36) Protects Intestinal Mucosal Immune Barrier in Sepsis. [Abstract]2015:2015:639412. PMID: 26346309
Solvent & Solubility
H2O : 100 mg/mL (Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL; Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Xu W, et al. A rapid and sensitive method for kinetic study and activity assay of DNase I in vitro based on a GO-quenched hairpin probe. Anal Bioanal Chem. 2016 May;408(14):3801-9. [Content Brief]
[2]. Rudin A, et al. Antibacterial activity of gentamicin sulfate in tissue culture. Appl Microbiol. 1970 Dec;20(6):989-90. [Content Brief]
[3]. Coopersmith CM, et al. Antibiotics improve survival and alter the inflammatory profile in a murine model of sepsis from Pseudomonas aeruginosa pneumonia. Shock. 2003 May;19(5):408-14. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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