Luminol
Based on 2 publication(s) in Google Scholar
Luminol is a chemical that exhibits chemiluminescence with pKa values of 6.74 and 15.1. Luminol exhibits chemiluminescence (CL) at 425 nm λmax. Luminol is commonly used in forensics as a diagnostic tool for the detection of blood stains.
For research use only. We do not sell to patients.
- Purity: 99.67%
- CAS No.: 521-31-3
- Formula: C8H7N3O2
- Molecular Weight:177.16
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Storage:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Luminol
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Biological Activity
Luminol concentration is an important factor that affects Chemiluminescence (CL) intensity. The intensity of Luminol chemiluminescence (LCL) does not depend on the concentration of Luminol alone, but also on other factors like concentration of oxidizing agents, enzymes, and pH. Maximum recorded CL intensity was at a Luminol concentration of 0.3 mM. CL intensity increased linearly with increasing concentrations of Luminol in the range of 0.01 to 0.3 mM. However, further increases of Luminol concentration caused a decrease in CL intensity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 521-31-3
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Appearance Solid
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Molecular Weight 177.16
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Formula C8H7N3O2
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Color Off-white to light yellow
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SMILES
O=C1NNC(C2=C1C=CC=C2N)=O
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Synonyms
Diogenes reagent
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Publications (2)
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Journal Impact Factor
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Most Recent
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J Inflamm Res
Kuiyangling Enema Alleviates Ulcerative Colitis Mice by Reducing Levels of Intestinal NETs and Promoting HuR/VDR Signaling. [Abstract]2025 Jan 8:18:381-403. PMID: 39802513 -
J Leukoc Biol
Transient receptor potential vanilloid 4 (TRPV4) in neutrophils enhances myocardial ischemia/reperfusion injury. [Abstract]2023 Sep 1;114(3):266-279. PMID: 37232941
Solvent & Solubility
DMSO : ≥ 30 mg/mL (169.34 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6446 mL | 28.2231 mL | 56.4461 mL | 141.1154 mL |
| 5 mM | 1.1289 mL | 5.6446 mL | 11.2892 mL | 28.2231 mL | |
| 10 mM | 0.5645 mL | 2.8223 mL | 5.6446 mL | 14.1115 mL | |
| 15 mM | 0.3763 mL | 1.8815 mL | 3.7631 mL | 9.4077 mL | |
| 20 mM | 0.2822 mL | 1.4112 mL | 2.8223 mL | 7.0558 mL | |
| 25 mM | 0.2258 mL | 1.1289 mL | 2.2578 mL | 5.6446 mL | |
| 30 mM | 0.1882 mL | 0.9408 mL | 1.8815 mL | 4.7038 mL | |
| 40 mM | 0.1411 mL | 0.7056 mL | 1.4112 mL | 3.5279 mL | |
| 50 mM | 0.1129 mL | 0.5645 mL | 1.1289 mL | 2.8223 mL | |
| 60 mM | 0.0941 mL | 0.4704 mL | 0.9408 mL | 2.3519 mL | |
| 80 mM | 0.0706 mL | 0.3528 mL | 0.7056 mL | 1.7639 mL | |
| 100 mM | 0.0564 mL | 0.2822 mL | 0.5645 mL | 1.4112 mL |
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