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  3. Fluorescent Dye
  4. 5-DTAF

5-DTAF is a fluorescein dye that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm. 5-DTAF can be used for biomolecular labeling, protein detection, and cell imaging.

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5-DTAF

5-DTAF 構造式

CAS 番号 : 51306-35-5

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 94 在庫あり
Solution
10 mM * 1 mL in DMSO USD 94 在庫あり
Solid
5 mg $86 在庫あり
10 mg $143 お問い合わせ
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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5-DTAF 関連抗体

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  • 生物活性

  • 純度とドキュメンテーション

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製品説明

5-DTAF is a fluorescein dye that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm. 5-DTAF can be used for biomolecular labeling, protein detection, and cell imaging[1].

体外実験

5-DTAF (10-5 mol/L; 1 h) acts as a fluorescent competitor for atrazine binding to Fe3O4-chitosan-based MMIP, with supernatant fluorescence intensity increases linearly correlated to the logarithm of atrazine concentration over 2.32-185.4 μM, enabling Atrazine (HY-N7091) detection[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Take cell labeling as an example:
(1) Wash the cells with PBS 3 times to remove serum and culture medium.
(2) Add serum-free medium containing 5-DTAF (1-5 μM), and incubate at 37°C in the dark for 30 minutes.
(3) Discard the staining solution, wash 3 times with preheated medium, and detect with a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-DTAF 関連抗体
分子量

495.27

分子式

C23H12Cl2N4O5
Emission (Em)

525
Excitation (Ex)

488
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(NC6=NC(Cl)=NC(Cl)=N6)C=C5
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (100.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0191 mL 10.0955 mL 20.1910 mL
5 mM 0.4038 mL 2.0191 mL 4.0382 mL
10 mM 0.2019 mL 1.0096 mL 2.0191 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション

純度: ≥94.0%

COA (258 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0191 mL 10.0955 mL 20.1910 mL 50.4775 mL
5 mM 0.4038 mL 2.0191 mL 4.0382 mL 10.0955 mL
10 mM 0.2019 mL 1.0096 mL 2.0191 mL 5.0478 mL
15 mM 0.1346 mL 0.6730 mL 1.3461 mL 3.3652 mL
20 mM 0.1010 mL 0.5048 mL 1.0096 mL 2.5239 mL
25 mM 0.0808 mL 0.4038 mL 0.8076 mL 2.0191 mL
30 mM 0.0673 mL 0.3365 mL 0.6730 mL 1.6826 mL
40 mM 0.0505 mL 0.2524 mL 0.5048 mL 1.2619 mL
50 mM 0.0404 mL 0.2019 mL 0.4038 mL 1.0096 mL
60 mM 0.0337 mL 0.1683 mL 0.3365 mL 0.8413 mL
80 mM 0.0252 mL 0.1262 mL 0.2524 mL 0.6310 mL
100 mM 0.0202 mL 0.1010 mL 0.2019 mL 0.5048 mL
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5-DTAF Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-DTAF51306-35-5Fluorescent DyeFluorescent Dye
Inhibitorinhibitorinhibit

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製品名:
5-DTAF
製品番号:
HY-D1082
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