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  4. 6-Aminofluorescein

6-Aminofluorescein (6-AF) is a fluorescent labeling reagent. 6-Aminofluorescein can be used to label fullerene-based liposome nanostructures termed ‘buckysomes′ (Ex/Em = 490/515 nm).

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6-Aminofluorescein

6-Aminofluorescein Estructura química

No. CAS : 51649-83-3

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Based on 1 publication(s) in Google Scholar

Other Forms of 6-Aminofluorescein:

6-Aminofluorescein Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 6-Aminofluorescein

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Descripciòn

6-Aminofluorescein (6-AF) is a fluorescent labeling reagent. 6-Aminofluorescein can be used to label fullerene-based liposome nanostructures termed ‘buckysomes′ (Ex/Em = 490/515 nm)[1].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Buckysomes are coupled to 6-Aminofluorescein
400 μL of buckysomes (2 mg/mL) is incubated with 100 μL each of 0.25 M EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (HY-D0178) and 0.25 M sulfo-NHS for 2 hrs at room temperature.
The pH is adjusted to 7.0 using NaOH.
300 μL of 6-Aminofluorescein (1 mg/mL prepared in DMSO) is added and incubated overnight at room temperature.
The free 6-Aminofluorescein is separated from 6-Aminofluorescein coupled amphiphilic fullerene monomer (AF-1) by size exclusion chromatography on Cross-linked dextran G 75 (HY-148139) column.
The fractions are analyzed by fluorometry for 6-Aminofluorescein emission at 520 nm.

Localization of 6-Aminofluorescein conjugated AF-1 using Fluorescence Microscopy
Human Coronary Artery Endothelial Cells are grown in 8-chamber tissue culture slides.
Exposed to 6-Aminofluorescein-buckysomes for 18 hrs at 37°C, 5%CO2.
Washes with Dulbecco's phosphate buffered saline.
Cells are fixed in 4% Paraformaldehyde for 20 min, and washed twice with Dulbecco's phosphate buffered saline.
Chambers are removed and slides are dried.
Fixed cells are mounted in antifade reagent with DAPI.
Observe using a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

6-Aminofluorescein Related Antibodies
Peso molecular

347.32

Fòrmula

C20H13NO5
No. CAS
Appearance

Solid

Color

Yellow to orange

Emission (Em)

515
Excitation (Ex)

490
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(N)=C5
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (287.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8792 mL 14.3959 mL 28.7919 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 98.84%

SDS (393 KB)

COA (256 KB) LCMS (94 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8792 mL 14.3959 mL 28.7919 mL 71.9797 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL 14.3959 mL
10 mM 0.2879 mL 1.4396 mL 2.8792 mL 7.1980 mL
15 mM 0.1919 mL 0.9597 mL 1.9195 mL 4.7986 mL
20 mM 0.1440 mL 0.7198 mL 1.4396 mL 3.5990 mL
25 mM 0.1152 mL 0.5758 mL 1.1517 mL 2.8792 mL
30 mM 0.0960 mL 0.4799 mL 0.9597 mL 2.3993 mL
40 mM 0.0720 mL 0.3599 mL 0.7198 mL 1.7995 mL
50 mM 0.0576 mL 0.2879 mL 0.5758 mL 1.4396 mL
60 mM 0.0480 mL 0.2399 mL 0.4799 mL 1.1997 mL
80 mM 0.0360 mL 0.1799 mL 0.3599 mL 0.8997 mL
100 mM 0.0288 mL 0.1440 mL 0.2879 mL 0.7198 mL
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6-Aminofluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Aminofluorescein51649-83-36-AFFluorescent DyeInhibitorinhibitorinhibit

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Nombre del producto:
6-Aminofluorescein
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HY-D0022A
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