2. Immunology/Inflammation
  3. CD73
  4. AB-680

AB-680 is a highly potent, reversible and selective inhibitor of CD73 (an ecto-nucleotidase), with a Ki of 4.9 pM for hCD73, displays >10,000-fold selectivity over related ecto-nucleotidases CD39. Anti-tumor activity.

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CAS 番号 : 2105904-82-1

容量 価格（税別） 在庫状況 数量
1 mg $142 在庫あり
5 mg $300 在庫あり
10 mg $480 在庫あり
25 mg $950 在庫あり
50 mg $1450 在庫あり
100 mg $2250 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 22 publication(s) in Google Scholar

Other Forms of AB-680:

AB-680 関連抗体

Top Publications Citing Use of Products

    AB-680 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Feb 17;85(4):692-703.  [Abstract]

    IFNγ levels measured by ELISA in supernatant of NY-ESO/624mel cultures exposed to AMP (300 μmol/L) with or without AB-680 (0.5 μmol/L) for 24 hous.

    AB-680 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Feb 17;85(4):692-703.  [Abstract]

    Percentage of labeled (M + 5) ADO and AMP out of total intracellular adenosine (ADO) in activated (1 ng/mL αCD3) NY-ESO-1–specific T cells exposed to isotopically labeled 15N5-AMP for 3 hours in presence or not of either AB-680 (0.5 μmol/L).

    AB-680 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2302498.  [Abstract]

    Image, volumes, and weights of the AB-680 (20 mg/kg; i.p.; once weekly for 3 weeks)‐treated versus Control KPC subcutaneous allografts (n = 6 samples per group).

    AB-680 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2302498.  [Abstract]

    Representative images of H&E staining, Masson's trichrome staining (MTS), and immunohistochemistry staining of CD8, Granzyme B, α‐SMA. Scale bar: 200 µm. The results showed that AB-680 (20 mg/kg; i.p.; once weekly for 3 weeks) significantly increased the numbers of CD8+ and Granzyme B+ cells.

    AB-680 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2302498.  [Abstract]

    Representative images of immunohistochemistry staining of CXCL5 and CXCR2 in the KPC tumors from the indicated treatment groups. The results showed that AB-680 (20 mg/kg, i.p.; once weekly) also increased the percentage of the corresponding receptor CXCR2+ area in treated tumors.

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    AB-680 is a highly potent, reversible and selective inhibitor of CD73 (an ecto-nucleotidase), with a Ki of 4.9 pM for hCD73, displays >10,000-fold selectivity over related ecto-nucleotidases CD39. Anti-tumor activity[1].

    IC50 & Target

    Ki: 4.9 pM (hCD73)[1]
    IC50: 43 pM (Soluble hCD73)[1]
    体外実験

    AB-680 (AB680) inhibits soluble hCD73 with an IC50 of 0.043 nM, also inhibits hCD73 in CHO cells, suppresses CD73 in human, mouse CD8+ T Cells and hPBMC, with IC50s of 0.070, 0.008, 0.66 and 0.011 nM, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AB-680 関連抗体
    臨床実験
    分子量

    580.82

    分子式

    C20H24ClFN4O9P2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=P(CP(O)(O)=O)(O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(Cl)C=C3N[C@H](C4=CC=CC=C4F)C)=C3C=N2)O1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (172.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (8.61 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7217 mL 8.6085 mL 17.2170 mL
    5 mM 0.3443 mL 1.7217 mL 3.4434 mL
    10 mM 0.1722 mL 0.8609 mL 1.7217 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.71% ee.: 99.96%

    COA (251 KB) HNMR (224 KB) RP-HPLC (111 KB) NP-HPLC (140 KB) LCMS (118 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.7217 mL 8.6085 mL 17.2170 mL 43.0426 mL
    5 mM 0.3443 mL 1.7217 mL 3.4434 mL 8.6085 mL
    DMSO 10 mM 0.1722 mL 0.8609 mL 1.7217 mL 4.3043 mL
    15 mM 0.1148 mL 0.5739 mL 1.1478 mL 2.8695 mL
    20 mM 0.0861 mL 0.4304 mL 0.8609 mL 2.1521 mL
    25 mM 0.0689 mL 0.3443 mL 0.6887 mL 1.7217 mL
    30 mM 0.0574 mL 0.2870 mL 0.5739 mL 1.4348 mL
    40 mM 0.0430 mL 0.2152 mL 0.4304 mL 1.0761 mL
    50 mM 0.0344 mL 0.1722 mL 0.3443 mL 0.8609 mL
    60 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7174 mL
    80 mM 0.0215 mL 0.1076 mL 0.2152 mL 0.5380 mL
    100 mM 0.0172 mL 0.0861 mL 0.1722 mL 0.4304 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    AB-680 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AB-6802105904-82-1AB680AB 680CD73ecto-5′-nucleotidase, NT5EInhibitorinhibitorinhibit

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    製品名:
    AB-680
    製品番号:
    HY-125286
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