1. Protein Tyrosine Kinase/RTK
  2. Trk Receptor


Cat. No.: HY-12497 Purity: 99.30%
Handling Instructions

ANA-12 is a potent and selective TrkB antagonist, and binds to TrkBECD-Fc with Kd of 12 μM.

For research use only. We do not sell to patients.

ANA-12 Chemical Structure

ANA-12 Chemical Structure

CAS No. : 219766-25-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 178 In-stock
Estimated Time of Arrival: December 31
10 mg USD 162 In-stock
Estimated Time of Arrival: December 31
50 mg USD 492 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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    ANA-12 purchased from MCE. Usage Cited in: Exp Eye Res. 2017 Jul 11;162:86-96.

    Blocking the BDNF pathway with ANA-12 prevents the protective effect of MDHB.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References


    ANA-12 is a potent and selective TrkB antagonist, and binds to TrkBECD-Fc with Kd of 12 μM.

    In Vitro

    ANA-12 (10 nM) prevents BDNF-induced neurite outgrowth in the TrkB-expressing cells, and completely abolishes the effects of BDNF at concentrations up to 10-100 μM[1].

    In Vivo

    ANA-12 (0.5 mg/kg, i.p.) partially inhibits the total endogenous TrkB activity in the whole brain of mice. ANA-12, injected in mice, demonstrates anxiolytic and antidepressive activities at 0.5 mg/kg. ANA-12 (0.5, 1.0, and 2.0 mg/kg) does not affect neuron survival[1]. ANA-12 (0.5 mg/kg) shows antidepressant effects in lipopolysaccharide (LPS)-induced depression-like behavior. ANA-12 (0.5 mg/kg) significantly attenuates an increased immobility time in depressed mice. In the TST, FST, and SPT, ANA-12 (0.5 mg/kg) does not show antidepressant-like effects in the control mice[2]. ANA-12 (0.5 mg/kg, i.p.) reverses the diminished self-administration of cocaine in CocSired rats[3].

    Solvent & Solubility
    In Vitro: 

    10 mM in DMSO

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4540 mL 12.2702 mL 24.5405 mL
    5 mM 0.4908 mL 2.4540 mL 4.9081 mL
    10 mM 0.2454 mL 1.2270 mL 2.4540 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      ANA-12 is prepared in vehicle of 17% DMSO in PBS[4].

    Animal Administration

    On the day of injection, ketamine (ketamine hydrochloride, 10 mg/kg), 7,8-dihydroxyflavone (7,8-DHF; 10 mg/kg), and ANA-12, N2-(2-{[(2-oxoazepan-3-yl) amino]carbonyl}phenyl)benzo[b]thiophene-2-carboxamide (0.5 mg/kg) are prepared in vehicle of 17 % dimethyl sulfoxide (DMSO) in phosphate-buffered saline. The doses of ketamine (10 mg/kg), 7,8-DHF (10 mg/kg), and ANA-12 (0.5 mg/kg) are selected. All compounds are administered intraperitoneally (i.p.) to mice.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.




    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month

    Room temperature in continental US; may vary elsewhere

    Purity: 99.30%

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    Cat. No.: HY-12497