1. Protein Tyrosine Kinase/RTK
  2. Trk Receptor

ANA-12 

Cat. No.: HY-12497 Purity: 99.30%
Handling Instructions

ANA-12 is a potent and selective TrkB antagonist with IC50s of 45.6 nM and 41.1 μM for the high and low affinity sites, respectively.

For research use only. We do not sell to patients.

ANA-12 Chemical Structure

ANA-12 Chemical Structure

CAS No. : 219766-25-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 178 In-stock
Estimated Time of Arrival: December 31
10 mg USD 162 In-stock
Estimated Time of Arrival: December 31
50 mg USD 492 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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    ANA-12 purchased from MCE. Usage Cited in: Neuroscience. 2018 Aug 4;388:357-366.

    Effects of ANA-12 on inflammatory cytokines, synaptic proteins, BDNF-TrkB signaling pathways in mice treated with Sulforaphane (SFN) plus Lipopolysaccharide (LPS).

    ANA-12 purchased from MCE. Usage Cited in: Exp Eye Res. 2017 Jul 11;162:86-96.

    Blocking the BDNF pathway with ANA-12 prevents the protective effect of MDHB.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    ANA-12 is a potent and selective TrkB antagonist with IC50s of 45.6 nM and 41.1 μM for the high and low affinity sites, respectively.

    In Vitro

    ANA-12 (10 nM) prevents BDNF-induced neurite outgrowth in the TrkB-expressing cells, and completely abolishes the effects of BDNF at concentrations up to 10-100 μM[1].

    In Vivo

    ANA-12 (0.5 mg/kg, i.p.) partially inhibits the total endogenous TrkB activity in the whole brain of mice. ANA-12, injected in mice, demonstrates anxiolytic and antidepressive activities at 0.5 mg/kg. ANA-12 (0.5, 1.0, and 2.0 mg/kg) does not affect neuron survival[1]. ANA-12 (0.5 mg/kg) shows antidepressant effects in lipopolysaccharide (LPS)-induced depression-like behavior. ANA-12 (0.5 mg/kg) significantly attenuates an increased immobility time in depressed mice. In the TST, FST, and SPT, ANA-12 (0.5 mg/kg) does not show antidepressant-like effects in the control mice[2]. ANA-12 (0.5 mg/kg, i.p.) reverses the diminished self-administration of cocaine in CocSired rats[3].

    Solvent & Solubility
    In Vitro: 

    DMSO : 9.09 mg/mL (22.31 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4540 mL 12.2702 mL 24.5405 mL
    5 mM 0.4908 mL 2.4540 mL 4.9081 mL
    10 mM 0.2454 mL 1.2270 mL 2.4540 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% saline

      Solubility: 1 mg/mL (2.45 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in saline)

      Solubility: ≥ 0.45 mg/mL (1.10 mM); Clear solution

    • 3.

      Add each solvent one by one:  5% DMSO    95% corn oil

      Solubility: ≥ 0.45 mg/mL (1.10 mM); Clear solution

    References
    Animal Administration
    [2]

    On the day of injection, ketamine (ketamine hydrochloride, 10 mg/kg), 7,8-dihydroxyflavone (7,8-DHF; 10 mg/kg), and ANA-12, N2-(2-{[(2-oxoazepan-3-yl) amino]carbonyl}phenyl)benzo[b]thiophene-2-carboxamide (0.5 mg/kg) are prepared in vehicle of 17 % dimethyl sulfoxide (DMSO) in phosphate-buffered saline. The doses of ketamine (10 mg/kg), 7,8-DHF (10 mg/kg), and ANA-12 (0.5 mg/kg) are selected. All compounds are administered intraperitoneally (i.p.) to mice.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    407.49

    Formula

    C₂₂H₂₁N₃O₃S

    CAS No.

    219766-25-3

    SMILES

    O=C(C1=CC2=CC=CC=C2S1)NC3=CC=CC=C3C(NC4C(NCCCC4)=O)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.30%

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    × = ×
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    Inquiry Information

    Product Name:
    ANA-12
    Cat. No.:
    HY-12497
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    ANA-12

    Cat. No.: HY-12497