1. Epigenetics
  2. AMPK
  3. Ampkinone


Cat. No.: HY-12831 Purity: 99.28%
COA Handling Instructions

Ampkinone is an indirect AMP-activated protein kinase (AMPK) activator.

For research use only. We do not sell to patients.

Ampkinone Chemical Structure

Ampkinone Chemical Structure

CAS No. : 1233082-79-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 214 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 214 In-stock
2 mg USD 108 In-stock
5 mg USD 192 In-stock
10 mg USD 360 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ampkinone

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review


Ampkinone is an indirect AMP-activated protein kinase (AMPK) activator.

IC50 & Target[1]



In Vitro

Ampkinone stimulates the phosphorylation of AMPK via the indirect activation of AMPK in various cell lines. Ampkinone-mediated activation of AMPK requires the activity of LKB1 and results in increased glucose uptake inmuscle cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ampkinone-treated DIO mice significantly reduce total body weight and overall fat mass. Histological examination and measurement of lipid parameters show that Ampkinone effectively improves metabolic abnormalities in the DIO mice model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight









Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (98.91 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9782 mL 9.8908 mL 19.7816 mL
5 mM 0.3956 mL 1.9782 mL 3.9563 mL
10 mM 0.1978 mL 0.9891 mL 1.9782 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (4.95 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.28%

Kinase Assay

Total AMPK activity is measured using a synthetic SAMS peptide substrate and [γ-32P]ATP. Briefly, 500 μg of protein extract is incubated with anti-AMPK-α1 and α2 antibodies for 2 h at 4°C. Protein A/G sepharose and agarose are added, and the mixtures are incubated for 3 h at 4°C. After the samples are washed three times with RIPA buffer, the activity is assessed in AMPK reaction buffer containing 20 mM HEPES-NaOH (pH 7.0), 0.4 mM Dithiothreitol, and 0.01% Brij-35, with or without 300 μM AMP. The immune complexes are added to 23 μL of reaction buffer per assay, and then 7 μL of SAMS substrate peptide (HMRSAMSGLHLVKRR, 100 μM final concentration) and 10 μL of ATP mixture (an aliquot of 1 μCi/μL: 90 μL of 75 mM magnesium chloride, 500 μM unlabeled ATP in 20 mM MOPS, pH7.2, 25 mMβ-glycerophosphate, 5 mM EGTA, 1 mM sodium orthovanadate, and 1 mM Dithiothreitol) are added, and the mixture is incubated at 30°C for 15 min. After 35 μL is spotted onto the center of P81 paper, the paper is washed three times with 0.75% phosphoric acid and once with acetone for 5 min. The samples are read using a scintillation counter[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

C57BL/6J mice are used and housed individually in a room maintained at 25°C on a 12/12 h light/dark schedule. For dietinduced obesity (DIO) mice, 4-week-old male C57BL/6J mice are fed a high-fat diet (HFD, 60% calories from fat) ad libitum for 8 weeks. Ampkinone in polyethylene glycol (PEG400) or vehicle is administered subcutaneously at 10 mg/kg body weight per day for 1 month. Body weight and food intake are measured every 3 days. All animals are given an insulin tolerance test (ITT) and sacrificed at 30 days. Liver weight and fat masses are measured, sectioned, and stained with Oil-red O and H&E.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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