β-Amyloid (25-35) (Synonyms: Amyloid beta-peptide (25-35); Aβ25-35; β-Amyloid peptide (25-35))

Name: β-Amyloid (25-35)
Brand: MedChemExpress
SKU: HY-P0128
Rating: 5.0 (42 reviews)

Cat. No.: HY-P0128 Purity: 99.20%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide, has shown neurotoxic activities in cultured cells.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 131602-53-4

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 42 publication(s) in Google Scholar

Other Forms of β-Amyloid (25-35):

FITC-β-Amyloid (25-35) In-stock
FITC-β-Ala-β-Amyloid (25-35） In-stock
β-Amyloid (25-35), HFIP-treated In-stock

42 Publications Citing Use of MCE β-Amyloid (25-35)

Cell Imaging/Staining

Flow Cytometry

ELISA

Bio/Physico-chemical Assay

Apoptosis Analysis

: β-Amyloid (25-35) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642. [Abstract]; DCFH-DA probe fluorescence Images of different groups of BV2 cells induced by β-Amyloid (25-35)(Aβ; 10µM; 24 h) , scale bar =250 μm.

: β-Amyloid (25-35) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642. [Abstract]; Flow cytometry detection of ROS in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells.

: β-Amyloid (25-35) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642. [Abstract]; Quantitative analysis of ELISA results for IL-1β, IL-6, TNF-α, and IL-10 in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells.

: β-Amyloid (25-35) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642. [Abstract]; Western blot analysis of IL-1β, IL-6, TNF-α, and IL-10 related pathological proteins in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells.

: β-Amyloid (25-35) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642. [Abstract]; Fluorescence images of IL-1β (I) and IL-6 (J) in different groups including β-Amyloid (25-35)(Aβ; 10µM; 24 h) of BV2 cells, scale bar = 50 μm.

: β-Amyloid (25-35) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642. [Abstract]; Quantitative analysis of fluorescence curves for Thioflavin T (THT) detection of Aβ fiber aggregation in different groups including induction by β-Amyloid (25-35) (Aβ; 20 µM; 0-72 h) of BV2 cells.

: β-Amyloid (25-35) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 19;22(1):642. [Abstract]; Image of Annexin V-FITC apoptosis detection in different groups including β-Amyloid (25-35) (Aβ; 10 µM; 24 h) of SH-SY5Y cells.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide, has shown neurotoxic activities in cultured cells^[1].

In Vitro

The amino acid sequence of Aβ(25-35) peptide is NH₂-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy^[1].
Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events^[2].
β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solid Aβ peptide is dissolved in cold hexafluoro-2-propanol (HFIP). The peptide is incubated at room temperature for at least 1 h to establish monomerization and randomization of structure.
2. The HFIP is removed by evaporation, and the resulting peptide is stored as a film at -20 or -80 °C.
3. The resulting film is dissolved in anhydrous DMSO at 5 mM and then dilutes into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.
4. Next, the solution is aged for 7 days at 37°C. The sample is then centrifuged at 14000g for 10 min at 4-8 °C; the soluble oligomers are in the supernatant. The supernatant is diluted 10-200-fold for experiments.
Methods vary depends on the downstream applications.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

β-Amyloid (25-35) can be used in animal modeling to create Alzheimer's disease models^[5].

Induction of Alzheimer's Disease (AD)^[5]

Background

The aggregation of β-Amyloid increases the concentration of Ca2+ in neurons, which is involved in regulating the phosphorylation and the hyperphosphorylation of Tau protein. Thus it results in neurofibrillary tangling and neuronal death, and eventually the animals gradually develop cognitive symptoms and develop Alzheimer's disease.

Specific Modeling Methods

Rat: Wistar • male • 180-200 g
Administration: 3 μg/μl in 5 μl within 1.2 μl/min • i.c.v • once

Note

(1) Animals are kept in a regulated environment (23± 1 °C,50%± 2% humidity) with 12 h light/dark cycle (light on 8:00 am-8:00 pm).
(2) Drug is given by injection using a G27 gauge needle inserted to a depth of 3.8 mm with a Hamilton syringe.

Modeling Indicators

Molecular changs: Aβ1-40 protein increase; malondialdehyde (MDA) increase; AChE and Bax expression decrease in hippocampal CA1 region.
Cognitive changes: Morris water maze test learning and memory deficits.

Correlated Product(s): /

Opposite Product(s): Aducanumab (HY-P9967); Donanemab (HY-P99859)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1060.27

Formula

C₄₅H₈₁N₁₃O₁₄S

CAS No.

131602-53-4

Appearance

Solid

Color

White to off-white

Sequence

Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met

Sequence Shortening

GSNKGAIIGLM

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : ≥ 3.33 mg/mL (3.14 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9432 mL	4.7158 mL	9.4316 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (243 KB) RP-HPLC (246 KB)

Handling Instructions (2659 KB)

References

[1]. D'Ursi AM, et al. Solution structure of amyloid beta-peptide (25-35) in different media. J Med Chem. 2004 Aug 12;47(17):4231-8. [Content Brief]

[2]. Clementi ME, et al. Abeta(31-35) and Abeta(25-35) fragments of amyloid beta-protein induce cellular death throughapoptotic signals: Role of the redox state of methionine-35. FEBS Lett. 2005 May 23;579(13):2913-8. [Content Brief]

[3]. Liu M, et al. Cognitive improvement of compound danshen in an Aβ25-35 peptide-induced rat model of Alzheimer's disease. BMC Complement Altern Med. 2015 Oct 23;15:382. [Content Brief]

[4]. Patel NS, et al. Potent anti-angiogenic motifs within the Alzheimer beta-amyloid peptide. Amyloid. 2008;15(1):5-19. [Content Brief]

[5]. Julia Fedotova, et al. Ropren® is a polyprenol preparation from coniferous plants that ameliorates cognitive deficiency in a rat model of beta-amyloid peptide-(25–35)-induced amnesia. Phytomedicine. 2012 Mar 15;19(5):451-6. [Content Brief]

Cell Assay ^[2]	Cell viability is determined by a modified MTS assay, which is based on the conversion of Tetrazolium salt by mitochondrial dehydrogenase to a formazan product spectrophotometrically measurable at 490 nm. PC12 cells are plated in 96-well plates at a density of 10 000 cells/well and maintained for 16 h in complete medium. Cells are then incubated in the absence (control) and presence of 40 μM Aβ(31-35) and Aβ(25-35) with reduced, oxidized and norleucine-substituted methionine-35 staurosporine 10 μM is used as positive control of 100% of cellular death. After 48 h of peptide-incubation, 20 μL of MTS reagent (2.0 mg/mL) is added to each well. The cells are then incubated for 30-45 min at 37 °C in a 5% CO₂ incubator. The reaction is stopped by adding 25 μL of 10% SDS. The plates are read with a microplate reader at 490 nm. Each data point is obtained using a triplet-well assay^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Rats^[3] Fifty-four Male Sprague-Dawley rats (2 months old, 300-350 g) are used. Amyloid beta-peptide(25-35) is dissolved in sterile distilled water at a concentration of 1 mg/mL as a stocking solution. Animals are infused with 5 μL/side of sterile distilled water (control), aggregated Aβ_25-35 (2 μg/μL), into bilateral cerebral lateral ventricles at a rate of 1 μL/min; the needle is left in place for 5 min. Then the needles are removed and rats are kept on a warm pad until they are awakened. To determine the neuroprotective effect on AD rats, the Aβ_25-35 treated rats are treated with CDS of different doses and Donepezil once daily for 23 days (including duration of behavior test). Experiment is performed to test the effect of CDS on Aβ_25-35-induced memory impairment using Morris water-maze and step-through passive avoidance tasks. Specifically, all of the rats are randomly divided into 6 groups for the experiment: (a) Vehicle 1 (for Aβ_25-35)+vehicle 2 (for CDS and Donepezil), (b) Aβ_25-35+vehicle 2, (c) Aβ_25-35+CDS (130 mg/kg), (d) Aβ_25-35+CDS (260 mg/kg), (e) Aβ_25-35+CDS (520 mg/kg), (f) Aβ_25-35+Donepezil (0.5 mg/kg). One day after cerebroventricular microinfusions of Aβ_25-35 (10 μg/side) or its vehicle, rats are treated (i.g.) with CDS or Donepezil or vehicle 2, once daily for 14 days prior to the beginning of Morris water maze, followed by passive avoidance task. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. D'Ursi AM, et al. Solution structure of amyloid beta-peptide (25-35) in different media. J Med Chem. 2004 Aug 12;47(17):4231-8. [Content Brief] [2]. Clementi ME, et al. Abeta(31-35) and Abeta(25-35) fragments of amyloid beta-protein induce cellular death throughapoptotic signals: Role of the redox state of methionine-35. FEBS Lett. 2005 May 23;579(13):2913-8. [Content Brief] [3]. Liu M, et al. Cognitive improvement of compound danshen in an Aβ25-35 peptide-induced rat model of Alzheimer's disease. BMC Complement Altern Med. 2015 Oct 23;15:382. [Content Brief] [4]. Patel NS, et al. Potent anti-angiogenic motifs within the Alzheimer beta-amyloid peptide. Amyloid. 2008;15(1):5-19. [Content Brief] [5]. Julia Fedotova, et al. Ropren® is a polyprenol preparation from coniferous plants that ameliorates cognitive deficiency in a rat model of beta-amyloid peptide-(25–35)-induced amnesia. Phytomedicine. 2012 Mar 15;19(5):451-6. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O

1 mM

0.9432 mL

4.7158 mL

9.4316 mL

23.5789 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

β-Amyloid (25-35) Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Amyloid (25-35)131602-53-4Amyloid beta-peptide (25-35) Aβ25-35β-Amyloid peptide (25-35)Amyloid-ββ-amyloid peptideAβAbetaInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

β-Amyloid (25-35) (Synonyms: Amyloid beta-peptide (25-35); Aβ25-35; β-Amyloid peptide (25-35))

Customer Review

Other Forms of β-Amyloid (25-35):

β-Amyloid (25-35) Related Antibodies

42 Publications Citing Use of MCE β-Amyloid (25-35)

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

β-Amyloid (25-35) Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

β-Amyloid (25-35) Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report