Aducanumab
Based on 1 Customer Validation
Aducanumab (BIIB037) is a human monoclonal antibody that selectively targets aggregated amyloid-beta (Aβ). Aducanumab shows blood-brain-barrier penetration, and can be used for Alzheimer's disease (AD) research.
For research use only. We do not sell to patients.
- Purity: 99.25%
- CAS No.: 1384260-65-4
- Molecular Weight:146.2 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Human IgG1 kappa
Human
Amyloid Beta/Aβ
Aducanumab (0.3-30 mg/kg, i.p., weekly, 6 months) reduces soluble and insoluble Aβ in a dose-dependent manner in 9.5- to 15.5-month-old Tg2576 transgenic mice[1].
Aducanumab (10 mg/kg, i.p., weekly, 6 months) restores intracellular calcium to control levels in 18-month-old Tg2576 mice[2].
Aducanumab (0.4-1 mg/mL, ICV, 20 min) leads to rapid decrease in amyloid burden, plaque clearance in Tg2576 mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:9.5- to 15.5-month-old Tg2576 transgenic mice [1]
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Dosage:0.3-30 mg/kg
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Administration:Intraperitoneal injection (i.p.), weekly, 6 months
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Result:Increased recruitment of Iba-1-positive microglia to Aβ plaques.
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Animal Model:18-month-old Tg2576 mice[2]
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Dosage:10 mg/kg
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Administration:Intraperitoneal injection (i.p.), weekly, 6 months
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Result:Restored neurite baseline calcium to control levels.
Decreased the number of neurites with elevated levels of calcium after 2 weeks.
Decreased the percentage of cell bodies with calcium overload.
Restored the levels of VILIP and SERCA to control levels.
Increased the cell numbers of NR1 and NR2A.
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Animal Model:22-month-old transgenic Tg2576 mice[2]
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Dosage:0.4-1 mg/mL
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Administration:Intracerebroventricular injection (ICV), 20 min
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Result:Decreased the number of amyloid plaque.
Decreased the size of the remaining individual plaques.
Reduced amyloid plaque burden.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG1 kappa
ELISA, FACS, Functional assay
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Immobilized Human Beta-amyloid/Beta-APP Protein (hFc Tag) can bind Aducanumab. The EC50 for this effect is 603.66 ng/mL. -
The EC50 for this effect is 603.66 ng/mL. Flow cytometric analysis of 1.5×106 HUVEC cells with Aducanumab (HY-P9967, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa, Isotype Control (HY-P99001, blue) was used as the isotype control.
Chemical Information
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CAS No. 1384260-65-4
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Appearance Liquid
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Molecular Weight 146.2 kDa
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Color Colorless to light yellow
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SMILES
[Aducanumab]
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Synonyms
BIIB037
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Sevigny J, et al. The antibody aducanumab reduces Aβ plaques in Alzheimer's disease. Nature. 2016 Sep 1;537(7618):50-6. [Content Brief]
[2]. Kastanenka KV, et al. Immunotherapy with Aducanumab Restores Calcium Homeostasis in Tg2576 Mice. J Neurosci. 2016 Dec 14;36(50):12549-12558. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)