B220
Based on 1 Customer Validation
B220 is an antiviral agent which can inhibit the growth of HSV-1, HSV-2 and human cytomegalovirus (CMV).
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 112228-65-6
- Formula: C20H22N4
- Molecular Weight:318.42
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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HSV-1 |
HSV-2 |
B220 is an antiviral agent which can inhibit the growth of HSV-1, HSV-2 and human cytomegalovirus (CMV)[1]. B220 inhibits neutrophil release of reactive oxygen species, as well as intracellular generation of reactive oxygen species. The inhibition is not achieved through direct oxygen radical scavenger activity of B220, and B220 has no immediate effects on the activity of the assembled oxidase. The neutrophil capability to phagocytose fluorescein-labeled opsonized yeast cells is reduced by B220. Cells preincubated with B220 (10 µg/mL) and then stimulated with the formylated peptide fMLP mobilize fewer C3 receptors on their surface than the control cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 112228-65-6
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Appearance Solid
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Molecular Weight 318.42
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Formula C20H22N4
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Color Light yellow to yellow
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SMILES
CC1=C(C)C=C2N=C3C(N(CCN(C)C)C4=C3C=CC=C4)=NC2=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Protocol
Cells preincubated with or without B220 are stimulated with N-formylmethionyl-leucyl-phenylalanine (fMLP) for 5 min and then fixed in paraformaldehyde [4% w/v in phosphate-buffered saline (PBS); 30 min on melting ice]. For detection of CR3, 10 µL of conjugated mAb is added to a cell pellet (approximately 100 µL) of 106 cells. The mixture is incubated for 30 min on ice and then washed twice with PBS. The amount of antibody bound is examined by flow cytometry. The release of vitamin B12-binding protein is assayed after activation of the cells with fMLP for 5 min using the cyanocobalamin technique[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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