2. Epigenetics
  3. Epigenetic Reader Domain
  4. BAY-850

BAY-850, a chemical probe, is a potent and isoform selective ATPase family AAA domain-containing protein 2 (ATAD2) inhibitor, with an IC50 of 166 nM.

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CAS 番号 : 2099142-76-2

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 173 在庫あり
Solution
10 mM * 1 mL in DMSO USD 173 在庫あり
Solid
5 mg $120 在庫あり
10 mg $180 在庫あり
25 mg $350 在庫あり
50 mg $550 在庫あり
100 mg $950 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 5 publication(s) in Google Scholar

Other Forms of BAY-850:

BAY-850 関連抗体

Top Publications Citing Use of Products

    BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601.  [Abstract]

    The effect of AM879, BAY-850 and compound 19f on ATAD2 activity detected by TR-FRET technology.

    BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601.  [Abstract]

    The effect of AM879, BAY-850 (10, 20, 30, 40, 50 μM), and compound 19f on the cell viability of BT-549 cells.

    BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601.  [Abstract]

    BT-549 cells were treated with 10 μM AM879, BAY-850 or compound 19f for 48 h, the expression level of p-c-Myc Ser62 was detected by immunofluorescence.

    BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601.  [Abstract]

    BT-549 cells were treated with 5, 10, 20 μM AM879, BAY-850, or compound 19f for 48 h, the expression level of ATAD2, c-Myc, and p-c-MycSer62 were detected by western blot.

    BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601.  [Abstract]

    Colony formation assay were used to detect the long-term cell proliferation ability after compound 19f, BAY-850 (10 μM) and AM879 treatment.

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    製品説明

    BAY-850, a chemical probe, is a potent and isoform selective ATPase family AAA domain-containing protein 2 (ATAD2) inhibitor, with an IC50 of 166 nM[1].

    IC50 & Target

    ATAD2[1].
    体外実験

    BAY-850 competes with the binding of a mono-acetylated Histone H4 N-terminal peptide to ATAD2 BD with an IC50 of 166 nM measured in TR-FRET assay. BAY-850 displaces the tetra-acetylated peptide with an IC50 of 157 nM and a KD of 115 nM respectively. The unprecedented isoform selectivity of BAY-850 suggests a different mode of action to those exhibited by canonical BD inhibitors[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BAY-850 関連抗体
    分子量

    654.24

    分子式

    C38H44ClN5O3
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(N[C@H](CC1=CC=C(C#N)C=C1)CN[C@H]2CC[C@@H](N)CC2)C3=CC(C4=CC=C(CN[C@@H](C5=CC=C(C)C=C5)C)O4)=C(OC)C=C3Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 62.5 mg/mL (95.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5285 mL 7.6425 mL 15.2849 mL
    5 mM 0.3057 mL 1.5285 mL 3.0570 mL
    10 mM 0.1528 mL 0.7642 mL 1.5285 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

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    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (7.64 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 5 mg/mL (7.64 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 98.93%

    COA (249 KB) HNMR (147 KB) RP-HPLC (141 KB) LCMS (108 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5285 mL 7.6425 mL 15.2849 mL 38.2123 mL
    5 mM 0.3057 mL 1.5285 mL 3.0570 mL 7.6425 mL
    10 mM 0.1528 mL 0.7642 mL 1.5285 mL 3.8212 mL
    15 mM 0.1019 mL 0.5095 mL 1.0190 mL 2.5475 mL
    20 mM 0.0764 mL 0.3821 mL 0.7642 mL 1.9106 mL
    25 mM 0.0611 mL 0.3057 mL 0.6114 mL 1.5285 mL
    30 mM 0.0509 mL 0.2547 mL 0.5095 mL 1.2737 mL
    40 mM 0.0382 mL 0.1911 mL 0.3821 mL 0.9553 mL
    50 mM 0.0306 mL 0.1528 mL 0.3057 mL 0.7642 mL
    60 mM 0.0255 mL 0.1274 mL 0.2547 mL 0.6369 mL
    80 mM 0.0191 mL 0.0955 mL 0.1911 mL 0.4777 mL
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    • Your name will appear on the site.

    BAY-850 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BAY-8502099142-76-2BAY850BAY 850Epigenetic Reader DomainInhibitorinhibitorinhibit

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    製品名:
    BAY-850
    製品番号:
    HY-119254
    数量:
    MCE 日本正規代理店:

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