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  4. Calcein

Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems.

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CAS. Nr. : 1461-15-0

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Based on 13 publication(s) in Google Scholar

Other Forms of Calcein:

Calcein Related Antibodies

Top Publications Citing Use of Products

    Calcein purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103804.

    Calcein fluorescence labeling showed a significantly lower rate of calcium salt deposition in the distal femur of 18Ms mice than in 3Ms mice. Five mice in each group were intraperitoneally injected with Calcein (10 mg/kg, ip) 10 days and 3 days before material extraction.

    Calcein purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103804.

    Five mice in each group were intraperitoneally injected with Calcein (10 mg/kg, ip) 10 days and 3 days before material extraction. Two calcium salt deposition lines were observed after non decalcified hard tissue sections, and the calcium salt deposition rate of the four groups of patients was measured and analyzed.

    Calcein purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 Jul 22:8:0784.  [Abstract]

    Representative images of Calcein double labeling in murine femur sections from designated groups; Scale bars, 20μm.

    Calcein purchased from MedChemExpress. Usage Cited in: Adv Mater. 2023 Jun;35(23):e2300548.  [Abstract]

    Cellular uptake of calcein by CT26 cells pretreated with EGCG, free DOX, Mal-DOX, NP, and pOEG-b-D-SH@NP for 5 h, respectively. Images for the Calcein fluorescence channel are shown in the inset boxes.

    Calcein purchased from MedChemExpress. Usage Cited in: Adv Mater. 2023 Jun;35(23):e2300548.  [Abstract]

    Fluorescence signals of Calcein in CT26 cells were recorded by flow cytometry.

    Calcein purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2022 Nov 15:16:3929-3946.

    Calcein and tetracycline labeling of mice femur at 8 weeks. The red arrow indicates the distance between calcein and tetracycline fluorescence labeling.

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    Beschreibung

    Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems[1][2].

    In Vitro

    Calcein is a fluorescent probe that emits yellow-green fluorescence and can be used to determine the labile iron pool as well as to measure the binding affinity of Fe(III) with ligands[3].
    Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
    1. Solution preparation[3]
    1.1 Preparation of stock solution
    Solvent: 1 M NaOH
    Concentration: 0.5 mM (optimize according to the experiment).
    Storage: Aliquot and store at -20°C or -80°C protected from light. Avoid repeated freeze-thaw cycles.
    1.2 Preparation of working solution
    Dilute to 0.5 μM with PBS or cell culture medium.
    Note: The working solution should be freshly prepared, used immediately, and protected from light.

    2. Cell staining
    2.1 Incubate cells (106/mL) with 20 μM Fe(II) at 37°C for 2 h.
    2.2 Collect cells by centrifugation (1000 rpm; 5 min), and stain by directly microinjecting Calcein (0.5 μM) into the cells (5-15 min).
    2.3 Wash cells twice with D'hanks buffer (pH 7.4).
    2.4 Monitor fluorescence using flow cytometry and confocal laser scanning microscopy.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Calcein Related Antibodies
    Molekulargewicht

    622.53

    Formel

    C30H26N2O13
    CAS. Nr.
    Appearance

    Solid

    Color

    Yellow to orange

    Emission (Em)

    515
    Excitation (Ex)

    489
    SMILES

    O=C1OC2(C3=C(OC4=C2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C4)C=C(O)C(CN(CC(O)=O)CC(O)=O)=C3)C5=C1C=CC=C5
    Versand

    Room temperature in continental US; may vary elsewhere.
    Speicherung

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Lösungsmittel & Löslichkeit
    In Vitro: 

    1M NaOH : 50 mg/mL (80.32 mM; Need ultrasonic)

    H2O : 5.65 mg/mL (9.08 mM; ultrasonic and warming and adjust pH to 8 with 1 M NaOH and heat to 60°C)

    DMSO : 2 mg/mL (3.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6063 mL 8.0317 mL 16.0635 mL
    5 mM 0.3213 mL 1.6063 mL 3.2127 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molaritätsrechner

    • Verdünnungsrechner

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Reinheit & Dokumentation

    SDS (393 KB)

    COA (254 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    Verweise
    Zellassay
    [1]

    Coelomocytes suspensions (ISO and CF) are separately incubated with calcein-AM (excitation and emission wavelength: 496 nm and 516 nm, respectively) at a final concentration of 200 nM, during 30 min, at 26°C. Calcein-AM is a nonfluorescent ABC transporter substrate whose intracellular accumulation is inversely proportional to ABC transporter activity. Intracellular esterases convert calcein-AM into the fluorescent dye calcein, which is not an ABC transporter substrate, thereby accumulating the dye inside the cell. Therefore, a high fluorescence signal indicates low ABC transporter activity whereas a low fluorescence signal indicates high activity. The fluorescence of samples is measured by flow cytometer. The experiment is repeated six times in duplicates.

    MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.
    Verweise

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O / 1M NaOH 1 mM 1.6063 mL 8.0317 mL 16.0635 mL 40.1587 mL
    H2O / 1M NaOH 5 mM 0.3213 mL 1.6063 mL 3.2127 mL 8.0317 mL
    1M NaOH 10 mM 0.1606 mL 0.8032 mL 1.6063 mL 4.0159 mL
    15 mM 0.1071 mL 0.5354 mL 1.0709 mL 2.6772 mL
    20 mM 0.0803 mL 0.4016 mL 0.8032 mL 2.0079 mL
    25 mM 0.0643 mL 0.3213 mL 0.6425 mL 1.6063 mL
    30 mM 0.0535 mL 0.2677 mL 0.5354 mL 1.3386 mL
    40 mM 0.0402 mL 0.2008 mL 0.4016 mL 1.0040 mL
    50 mM 0.0321 mL 0.1606 mL 0.3213 mL 0.8032 mL
    60 mM 0.0268 mL 0.1339 mL 0.2677 mL 0.6693 mL
    80 mM 0.0201 mL 0.1004 mL 0.2008 mL 0.5020 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Calcein Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Calcein1461-15-0FluorexonFluorescent DyeInhibitorinhibitorinhibit

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