Folinic acid calcium
Based on 17 publication(s) in Google Scholar
Folinic acid calcium (Leucovorin calcium) is a biological folic acid and is generally administered along with methotrexate (MTX) as a rescue agent to decrease MTX-induced toxicity.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 1492-18-8
- Formula: C20H21CaN7O7
- Molecular Weight:511.50
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Storage:
4°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Folinic acid calcium
More- Signal Transduct Target Ther. 2025 Dec 15;10(1):406. [Abstract]
- Cancer Cell. 2026 Mar 9;44(3):658-675.e12. [Abstract]
- JAMA Oncol. 2022 Jan 1;8(1):e215445. [Abstract]
- Nat Commun. 2020 Apr 14;11(1):1792. [Abstract]
- EBioMedicine. 2024 Apr:102:105041. [Abstract]
- NPJ Precis Oncol. 2023 Dec 8;7(1):128. [Abstract]
- Mol Cancer Ther. 2025 Dec 4. [Abstract]
- Mol Oncol. 2026 Jun 5. [Abstract]
- Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]
- Appl Microbiol Biotechnol. 2024 Dec;108(1):1-15. [Abstract]
- J Mol Med (Berl). 2019 Aug;97(8):1183-1193. [Abstract]
- Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]
- Virology. 2023 Aug:585:205-214. [Abstract]
- Oncol Lett. 2026 Mar 4;31(5):155. [Abstract]
- bioRxiv. 2025 Sep 17.
- Patent. US20230101335A1.
- bioRxiv. 2023 Feb 28.
Customer Validation & Images
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Flow Cytometry
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Flow Cytometry
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WB
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In Vivo Efficacy Study
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
MTX alone induces a concentration-related increase in % micronucleated binucleated cells (MNBN) and % aberrant cells (Abs). There is a decrease in nuclear division index (NDI) with increase in MTX concentration. Similarly, the mitotic index (MI) also decreases in all concentrations of MTX tested. The addition of Folinic acid at 50 μg/ mL significantly reduces % MNBN (40-68%) and % Abs (36-77%). Inhibition is also seen at 5 μg/ mL Folinic acid (12 to 54% for MNBN and 20 to 61% for Abs) [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:24 Balb/c young growing male mice aged 3 weeks (11.88 ± 0.25 g)[2]
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Dosage:7.0 mg/kg
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Administration:Intraperitoneal injection; every second day; for 3 weeks
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Result:Following methotrexate (MTX) administration appears to reverse this growth inhibition.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1492-18-8
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Appearance Solid
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Molecular Weight 511.50
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Formula C20H21CaN7O7
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Color Off-white to light yellow
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SMILES
O=C(N[C@H](C([O-])=O)CCC([O-])=O)C(C=C1)=CC=C1NCC(N2C=O)CNC3=C2C(N=C(N)N3)=O.[Ca+2]
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Synonyms
Leucovorin calcium; Calcium folinate
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (17)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]2025 Dec 15;10(1):406. PMID: 41392286 -
Cancer Cell
Chemotherapy triggers immune evasion by fostering LEPR+ Kupffer cell differentiation in liver metastases. [Abstract]2026 Mar 9;44(3):658-675.e12. PMID: 41687606 -
JAMA Oncol
Chemoradiotherapy Plus Induction or Consolidation Chemotherapy as Total Neoadjuvant Therapy for Patients With Locally Advanced Rectal Cancer: Long-term Results of the CAO/ARO/AIO-12 Randomized Clinical Trial. [Abstract]2022 Jan 1;8(1):e215445. PMID: 34792531 -
Nat Commun
Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. [Abstract]2020 Apr 14;11(1):1792. PMID: 32286289 -
EBioMedicine
Desulfovibrio desulfuricans and its derived metabolites confer resistance to FOLFOX through METTL3. [Abstract]2024 Apr:102:105041. PMID: 38484555 -
NPJ Precis Oncol
Single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer. [Abstract]2023 Dec 8;7(1):128. PMID: 38066116 -
Mol Cancer Ther
Mosperafenib, a novel paradox breaker BRAF inhibitor with potent preclinical activity in BRAF mutated colorectal cancer. [Abstract]2025 Dec 4. PMID: 41340484 -
Mol Oncol
Patient therapy outcome modeling in cancer organoids is improved by cancer-associated fibroblasts and organoid assembly convolution. [Abstract]2026 Jun 5. PMID: 42246237 -
Mol Oncol
Optimized low-dose combinatorial drug treatment boosts selectivity and efficacy of colorectal carcinoma treatment. [Abstract]2020 Nov;14(11):2894-2919. PMID: 33021054
Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]
FOLFOX was administered as 6 mg/kg Oxaliplatin two hours before administration of 90 mg/kg Leucovorin and 50 mg/kg 5-Fluorouracil at day 1 and a repeat of 5‐Fluorouracil dosing on day 2. Weight loss of ODC-, CTRL-, and FOLFOX-treated mice over time of mice with DLD1 tumors.
Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]
The corresponding panels show the activity of ODC, the corresponding monotherapy, and FOLFOX (0.5 µM Leucovorin, 5-Fluorouracil, Oxaliplatin), measured by comparing ATP levels in CRC cells (colored bars) and healthy CCD841 colonic epithelial cells (black bars) with CTRL (<0.15% DMSO), thus obtaining the treatment window (TW).
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Appl Microbiol Biotechnol
Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model. [Abstract]2024 Dec;108(1):1-15. PMID: 38175236 -
J Mol Med (Berl)
2019 Aug;97(8):1183-1193. PMID: 31201471 -
Int J Immunopathol Pharmacol
Chemotherapy elevates cell surface PD-L1 and MHC-I expression in apoptotic gastric cancer cells. [Abstract]2025 Jan-Dec:39:3946320251338662. PMID: 40380807
Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]
Effects of FOLFOX on surface PD-L1 expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and PD-L1 antibody. Representative histogram showing surface PD-L1 expression. Red histogram: total cells. Purple histogram: annexin V-positive cells.
Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]
Effects of FOLFOX on surface MHC-I expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and MHC-I antibody. Representative dot plots showing the expression of annexin V and surface MHC-I on AGS cells.
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Virology
Structure-based virtual screening of ROCK1 inhibitors for the discovery of Enterovirus-A71 antivirals. [Abstract]2023 Aug:585:205-214. PMID: 37384967
Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Virology. 2023 Aug:585:205-214. [Abstract]
Leucovorin (10 μM; 48 h). VP1 protein levels were detected using Western blot.
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Oncol Lett
Leucovorin enhances curcumin-induced inhibition of cell viability and ferroptosis in colorectal cancer cells. [Abstract]2026 Mar 4;31(5):155. PMID: 41852897 -
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Solvent & Solubility
H2O : 50 mg/mL (97.75 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Keshava C, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutat Res. 1998 Feb 2;397(2):221-8. [Content Brief]
[2]. Iqbal MP, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatr. 2003 Dec;92(12):1438-44. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 1.9550 mL | 9.7752 mL | 19.5503 mL | 48.8759 mL |
| 5 mM | 0.3910 mL | 1.9550 mL | 3.9101 mL | 9.7752 mL | |
| 10 mM | 0.1955 mL | 0.9775 mL | 1.9550 mL | 4.8876 mL | |
| 15 mM | 0.1303 mL | 0.6517 mL | 1.3034 mL | 3.2584 mL | |
| 20 mM | 0.0978 mL | 0.4888 mL | 0.9775 mL | 2.4438 mL | |
| 25 mM | 0.0782 mL | 0.3910 mL | 0.7820 mL | 1.9550 mL | |
| 30 mM | 0.0652 mL | 0.3258 mL | 0.6517 mL | 1.6292 mL | |
| 40 mM | 0.0489 mL | 0.2444 mL | 0.4888 mL | 1.2219 mL | |
| 50 mM | 0.0391 mL | 0.1955 mL | 0.3910 mL | 0.9775 mL | |
| 60 mM | 0.0326 mL | 0.1629 mL | 0.3258 mL | 0.8146 mL | |
| 80 mM | 0.0244 mL | 0.1222 mL | 0.2444 mL | 0.6109 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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