2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. Antifolate Endogenous Metabolite
  4. Folinic acid calcium

Folinic acid calcium  (Synonyms: Leucovorin calcium; Calcium folinate)

Cat. No.: HY-13664 Purity: 99.86%
COA
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Handling Instructions Technical Support

Folinic acid calcium (Leucovorin calcium) is a biological folic acid and is generally administered along with methotrexate (MTX) as a rescue agent to decrease MTX-induced toxicity.

For research use only. We do not sell to patients.

CAS No. : 1492-18-8

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Customer Review

Based on 16 publication(s) in Google Scholar

Other Forms of Folinic acid calcium:

Folinic acid calcium Related Antibodies

Top Publications Citing Use of Products

    Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662.  [Abstract]

    Effects of FOLFOX on surface PD-L1 expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and PD-L1 antibody. Representative histogram showing surface PD-L1 expression. Red histogram: total cells. Purple histogram: annexin V-positive cells.

    Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662.  [Abstract]

    Effects of FOLFOX on surface MHC-I expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and MHC-I antibody. Representative dot plots showing the expression of annexin V and surface MHC-I on AGS cells.

    Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Virology. 2023 Aug:585:205-214.

    Leucovorin (10 μM; 48 h). VP1 protein levels were detected using Western blot.

    Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919.  [Abstract]

    FOLFOX was administered as 6 mg/kg Oxaliplatin two hours before administration of 90 mg/kg Leucovorin and 50 mg/kg 5-Fluorouracil at day 1 and a repeat of 5‐Fluorouracil dosing on day 2. Weight loss of ODC-, CTRL-, and FOLFOX-treated mice over time of mice with DLD1 tumors.

    Folinic acid calcium purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919.  [Abstract]

    The corresponding panels show the activity of ODC, the corresponding monotherapy, and FOLFOX (0.5 µM Leucovorin, 5-Fluorouracil, Oxaliplatin), measured by comparing ATP levels in CRC cells (colored bars) and healthy CCD841 colonic epithelial cells (black bars) with CTRL (<0.15% DMSO), thus obtaining the treatment window (TW).

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Folinic acid calcium (Leucovorin calcium) is a biological folic acid and is generally administered along with methotrexate (MTX) as a rescue agent to decrease MTX-induced toxicity[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    MTX alone induces a concentration-related increase in % micronucleated binucleated cells (MNBN) and % aberrant cells (Abs). There is a decrease in nuclear division index (NDI) with increase in MTX concentration. Similarly, the mitotic index (MI) also decreases in all concentrations of MTX tested. The addition of Folinic acid at 50 μg/ mL significantly reduces % MNBN (40-68%) and % Abs (36-77%). Inhibition is also seen at 5 μg/ mL Folinic acid (12 to 54% for MNBN and 20 to 61% for Abs) [1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Folinic acid calcium Related Antibodies
    In Vivo

    Folinic acid (7.0 mg/kg; intraperitoneal injection; every second day; for 3 weeks; Balb/c young growing male mice) treatment following methotrexate (MTX) administration appears to reverse this growth inhibition (Chronic administration of MTX induces suppression of skeletal growth in mice)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 24 Balb/c young growing male mice aged 3 weeks (11.88 ± 0.25 g)[2]
    Dosage: 7.0 mg/kg
    Administration: Intraperitoneal injection; every second day; for 3 weeks
    Result: Following methotrexate (MTX) administration appears to reverse this growth inhibition.
    Clinical Trial
    Molecular Weight

    511.50

    Formula

    C20H21CaN7O7
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(N[C@H](C([O-])=O)CCC([O-])=O)C(C=C1)=CC=C1NCC(N2C=O)CNC3=C2C(N=C(N)N3)=O.[Ca+2]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (97.75 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9550 mL 9.7752 mL 19.5503 mL
    5 mM 0.3910 mL 1.9550 mL 3.9101 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.86%

    SDS (393 KB)

    COA (248 KB) RP-HPLC (247 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.9550 mL 9.7752 mL 19.5503 mL 48.8759 mL
    5 mM 0.3910 mL 1.9550 mL 3.9101 mL 9.7752 mL
    10 mM 0.1955 mL 0.9775 mL 1.9550 mL 4.8876 mL
    15 mM 0.1303 mL 0.6517 mL 1.3034 mL 3.2584 mL
    20 mM 0.0978 mL 0.4888 mL 0.9775 mL 2.4438 mL
    25 mM 0.0782 mL 0.3910 mL 0.7820 mL 1.9550 mL
    30 mM 0.0652 mL 0.3258 mL 0.6517 mL 1.6292 mL
    40 mM 0.0489 mL 0.2444 mL 0.4888 mL 1.2219 mL
    50 mM 0.0391 mL 0.1955 mL 0.3910 mL 0.9775 mL
    60 mM 0.0326 mL 0.1629 mL 0.3258 mL 0.8146 mL
    80 mM 0.0244 mL 0.1222 mL 0.2444 mL 0.6109 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Folinic acid calcium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Folinic acid1492-18-8Leucovorin Calcium folinateAntifolateEndogenous MetaboliteInhibitorinhibitorinhibit

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