2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. Antifolate Endogenous Metabolite
  4. Folinic acid calcium salt pentahydrate

Folinic acid calcium salt pentahydrate  (Synonyms: Leucovorin calcium salt pentahydrate)

Cat. No.: HY-B0080 Purity: 98.0%
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Folinic acid calcium salt pentahydrate (Leucovorin calcium salt pentahydrate) is a biological folic acid and is generally administered along with methotrexate (MTX) as a rescue agent to decrease MTX-induced toxicity.

For research use only. We do not sell to patients.

CAS No. : 6035-45-6

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Based on 16 publication(s) in Google Scholar

Other Forms of Folinic acid calcium salt pentahydrate:

Folinic acid calcium salt pentahydrate Related Antibodies

Top Publications Citing Use of Products

    Folinic acid calcium salt pentahydrate purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662.  [Abstract]

    Effects of FOLFOX on surface PD-L1 expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and PD-L1 antibody. Representative histogram showing surface PD-L1 expression. Red histogram: total cells. Purple histogram: annexin V-positive cells.

    Folinic acid calcium salt pentahydrate purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662.  [Abstract]

    Effects of FOLFOX on surface MHC-I expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and MHC-I antibody. Representative dot plots showing the expression of annexin V and surface MHC-I on AGS cells.

    Folinic acid calcium salt pentahydrate purchased from MedChemExpress. Usage Cited in: Virology. 2023 Aug:585:205-214.

    Leucovorin (10 μM; 48 h). VP1 protein levels were detected using Western blot.

    Folinic acid calcium salt pentahydrate purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919.  [Abstract]

    FOLFOX was administered as 6 mg/kg Oxaliplatin two hours before administration of 90 mg/kg Leucovorin and 50 mg/kg 5-Fluorouracil at day 1 and a repeat of 5‐Fluorouracil dosing on day 2. Weight loss of ODC-, CTRL-, and FOLFOX-treated mice over time of mice with DLD1 tumors.

    Folinic acid calcium salt pentahydrate purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919.  [Abstract]

    The corresponding panels show the activity of ODC, the corresponding monotherapy, and FOLFOX (0.5 µM Leucovorin, 5-Fluorouracil, Oxaliplatin), measured by comparing ATP levels in CRC cells (colored bars) and healthy CCD841 colonic epithelial cells (black bars) with CTRL (<0.15% DMSO), thus obtaining the treatment window (TW).

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    Description

    Folinic acid calcium salt pentahydrate (Leucovorin calcium salt pentahydrate) is a biological folic acid and is generally administered along with methotrexate (MTX) as a rescue agent to decrease MTX-induced toxicity[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    MTX alone induces a concentration-related increase in % micronucleated binucleated cells (MNBN) and % aberrant cells (Abs). There is a decrease in nuclear division index (NDI) with increase in MTX concentration. Similarly, the mitotic index (MI) also decreases in all concentrations of MTX tested. The addition of Folinic acid at 50 μg/ mL significantly reduces % MNBN (40-68%) and % Abs (36-77%). Inhibition is also seen at 5 μg/ mL Folinic acid (12 to 54% for MNBN and 20 to 61% for Abs) [1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Folinic acid calcium salt pentahydrate Related Antibodies
    In Vivo

    Folinic acid (7.0 mg/kg; intraperitoneal injection; every second day; for 3 weeks; Balb/c young growing male mice) treatment following methotrexate (MTX) administration appears to reverse this growth inhibition (Chronic administration of MTX induces suppression of skeletal growth in mice)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 24 Balb/c young growing male mice aged 3 weeks (11.88 ± 0.25 g)[2]
    Dosage: 7.0 mg/kg
    Administration: Intraperitoneal injection; every second day; for 3 weeks
    Result: Following methotrexate (MTX) administration appears to reverse this growth inhibition.
    Molecular Weight

    601.58

    Formula

    C20H31CaN7O12
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=CN1C(C2=O)=C(NC(N)=N2)NCC1CNC3=CC=C(C(N[C@H](C([O-])=O)CCC([O-])=O)=O)C=C3.O.O.O.O.[Ca+2].O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    H2O : 10 mg/mL (16.62 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6623 mL 8.3114 mL 16.6229 mL
    5 mM 0.3325 mL 1.6623 mL 3.3246 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.6623 mL 8.3114 mL 16.6229 mL 41.5572 mL
    5 mM 0.3325 mL 1.6623 mL 3.3246 mL 8.3114 mL
    10 mM 0.1662 mL 0.8311 mL 1.6623 mL 4.1557 mL
    15 mM 0.1108 mL 0.5541 mL 1.1082 mL 2.7705 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Folinic acid calcium salt pentahydrate Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Folinic acid calcium salt6035-45-6Leucovorin calcium saltAntifolateEndogenous MetaboliteInhibitorinhibitorinhibit

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