Aminopterin (Synonyms: 4-Aminofolic acid; APGA)

Name: Aminopterin
Brand: MedChemExpress
SKU: HY-14518
Rating: 4.5 (0 reviews)

Cat. No.: HY-14518 Purity: 98.67%: Data Sheet
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Aminopterin (4-Aminofolic acid), the 4-amino derivative of folic acid, is a folic acid antagonist. Aminopterin catalyses the reduction of folic acid to tetrahydrofolic acid, and competitively inhibits dihydrofolate reductase (DHFR) with a K_i of 3.7 pM. Aminopterin has anticancer and immunosuppressive activity. Aminopterin is used in treatment of pediatric leukemia.

For research use only. We do not sell to patients.

Aminopterin Chemical Structure

CAS No. : 54-62-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Other Forms of Aminopterin:

Aminopterin sodium Get quote
Aminopterin (Standard) Get quote

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Biological Activity
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
L1210	IC₅₀	0.72 1 Compound: AMT	Compound was evaluated for inhibitory effect on growth of L1210 cells at IC50 (n=4) Compound was evaluated for inhibitory effect on growth of L1210 cells at IC50 (n=4)	[PMID: 2423690]
CCRF-CEM	IC₅₀	0.001 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against human leukemic lymphoblast CEM cells Tested for cell-growth inhibition against human leukemic lymphoblast CEM cells	[PMID: 2898531]
CCRF-CEM	IC₅₀	3 1 Compound: AMT	Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target	[PMID: 8035423]
CCRF-CEM	IC₅₀	0.001 3 Compound: AMT	Cell growth inhibition against CEM cell from human leukemic lymphoblasts Cell growth inhibition against CEM cell from human leukemic lymphoblasts	[PMID: 3872941]
CCRF-CEM	IC₅₀	3 3 Compound: AMT	Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM) Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM)	[PMID: 8568828]
CCRF-CEM	IC₅₀	0.001 3 Compound: AMT	Cell growth inhibition against CEM cell from human leukemic lymphoblasts Cell growth inhibition against CEM cell from human leukemic lymphoblasts	[PMID: 3872941]
CCRF-CEM	IC₅₀	0.32 3 Compound: AMT	Cell growth inhibition against CEM/MTX cell from human leukemic lymphoblasts Cell growth inhibition against CEM/MTX cell from human leukemic lymphoblasts	[PMID: 3872941]
CCRF-CEM	IC₅₀	0.32 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against human leukemic lymphoblast CEM/MTX cells Tested for cell-growth inhibition against human leukemic lymphoblast CEM/MTX cells	[PMID: 2898531]
HeLa	IC₅₀	3.6 1 Compound: AMT	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
CCRF-CEM	IC₅₀	0.32 3 Compound: AMT	Cell growth inhibition against CEM/MTX cell from human leukemic lymphoblasts Cell growth inhibition against CEM/MTX cell from human leukemic lymphoblasts	[PMID: 3872941]
CCRF-CEM	IC₅₀	3 3 Compound: AMT	Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM) Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM)	[PMID: 8568828]
HeLa	IC₅₀	8.3 1 Compound: AMT	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
CCRF-CEM	IC₅₀	3 1 Compound: AMT	Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target	[PMID: 8035423]
CCRF-CEM	IC₅₀	0.001 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against human leukemic lymphoblast CEM cells Tested for cell-growth inhibition against human leukemic lymphoblast CEM cells	[PMID: 2898531]
HL-60	IC₅₀	2.5 1 Compound: AMT	Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
CCRF-CEM	IC₅₀	3 3 Compound: AMT	Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM) Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM)	[PMID: 8568828]
CCRF-CEM	IC₅₀	0.32 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against human leukemic lymphoblast CEM/MTX cells Tested for cell-growth inhibition against human leukemic lymphoblast CEM/MTX cells	[PMID: 2898531]
HL-60	IC₅₀	3 1 Compound: AMT	Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
CCRF-CEM	IC₅₀	4.4 1 Compound: 1	Growth inhibition of CCRF-CEM human leukemic lymphoblasts Growth inhibition of CCRF-CEM human leukemic lymphoblasts	[PMID: 10780919]
L1210	IC₅₀	0.002 3 Compound: 2	Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure. Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure.	[PMID: 1992118]
L1210	IC₅₀	1.2 1 Compound: Aminopterin	In vitro inhibition of L1210 cell growth in rodent neoplastic cells In vitro inhibition of L1210 cell growth in rodent neoplastic cells	[PMID: 7108907]
L1210	EC₅₀	1.8 1 Compound: aminopterin	Inhibition of growth (cytotoxicity) of L1210 cell line in vitro Inhibition of growth (cytotoxicity) of L1210 cell line in vitro	[PMID: 6410066]
L1210	IC₅₀	2 1 Compound: aminopterin(AMT)	Compound was evaluated for the growth inhibition of L1210 cells. Compound was evaluated for the growth inhibition of L1210 cells.	[PMID: 1995880]
HL-60	IC₅₀	2.5 1 Compound: AMT	Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
CCRF-CEM	IC₅₀	3 1 Compound: AMT	Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target	[PMID: 8035423]
HL-60	IC₅₀	3 1 Compound: AMT	Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
HeLa	IC₅₀	3.6 1 Compound: AMT	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
SCC-7	IC₅₀	4 1 Compound: AMT	Tested for inhibitory concentration against SCC-VII murine squamous carcinoma cell line Tested for inhibitory concentration against SCC-VII murine squamous carcinoma cell line	[PMID: 8035423]
Detroit 98	IC₅₀	0.002 3 Compound: Aminopterin	Evaluated in vitro for ability to inhibit the Detroit 98 cell lines Evaluated in vitro for ability to inhibit the Detroit 98 cell lines	[PMID: 2299624]
CCRF-CEM	IC₅₀	4.4 1 Compound: 1	Growth inhibition of CCRF-CEM human leukemic lymphoblasts Growth inhibition of CCRF-CEM human leukemic lymphoblasts	[PMID: 10780919]
HuTu80	EC₅₀	4.7 1 Compound: aminopterin	Inhibition of growth (cytotoxicity) of HuTu 80 cell line in vitro Inhibition of growth (cytotoxicity) of HuTu 80 cell line in vitro	[PMID: 6410066]
Detroit 98	IC₅₀	0.002 3 Compound: Aminopterin	Evaluated in vitro for ability to inhibit the Detroit 98 cell lines Evaluated in vitro for ability to inhibit the Detroit 98 cell lines	[PMID: 2299624]
HL-60	IC₅₀	2.5 1 Compound: AMT	Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
SCC-25	IC₅₀	6.9 1 Compound: AMT	Tested for inhibitory concentration against human SCC25 cell line Tested for inhibitory concentration against human SCC25 cell line	[PMID: 8035423]
HL-60	IC₅₀	3 1 Compound: AMT	Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
HeLa	IC₅₀	8.3 1 Compound: AMT	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
HeLa	IC₅₀	3.6 1 Compound: AMT	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
HeLa	IC₅₀	31 1 Compound: aminopterin	Inhibition of dihydrofolate reductase in HeLa cells Inhibition of dihydrofolate reductase in HeLa cells	[PMID: 6410066]
HeLa	IC₅₀	31 1 Compound: aminopterin	Inhibition of dihydrofolate reductase in HeLa cells Inhibition of dihydrofolate reductase in HeLa cells	[PMID: 6410066]
KB	ED₅₀	0.003 6 Compound: 2	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 448685]
HeLa	IC₅₀	8.3 1 Compound: AMT	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay	[PMID: 31843461]
L1210	IC₅₀	0.002 3 Compound: 2	Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure. Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure.	[PMID: 1992118]
L1210	IC₅₀	0.002 3 Compound: AMT	Cell growth inhibition against mouse leukemia L1210 cells Cell growth inhibition against mouse leukemia L1210 cells	[PMID: 3872941]
HuTu80	EC₅₀	4.7 1 Compound: aminopterin	Inhibition of growth (cytotoxicity) of HuTu 80 cell line in vitro Inhibition of growth (cytotoxicity) of HuTu 80 cell line in vitro	[PMID: 6410066]
L1210	IC₅₀	7.9 3 Compound: AMT	Cell growth inhibition against mouse leukemia L1210/R71 cells Cell growth inhibition against mouse leukemia L1210/R71 cells	[PMID: 3872941]
KB	ED₅₀	0.003 6 Compound: 2	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 448685]
L1210	IC₅₀	84 3 Compound: AMT	Cell growth inhibition against mouse leukemia L1210/R81 cells Cell growth inhibition against mouse leukemia L1210/R81 cells	[PMID: 3872941]
L1210	IC₅₀	0.002 3 Compound: Aminopterin	Compound was evaluated for cell growth inhibition of murine (L1210) leukemic cells Compound was evaluated for cell growth inhibition of murine (L1210) leukemic cells	[PMID: 1992122]
L1210	EC₅₀	1.8 1 Compound: aminopterin	Inhibition of growth (cytotoxicity) of L1210 cell line in vitro Inhibition of growth (cytotoxicity) of L1210 cell line in vitro	[PMID: 6410066]
L1210	IC₅₀	0.002 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against mouse leukemic L1210 cells Tested for cell-growth inhibition against mouse leukemic L1210 cells	[PMID: 2898531]
L1210	IC₅₀	0.002 3 Compound: AMT	Compound was tested for its ability to inhibit growth of L1210 mouse leukemia cells Compound was tested for its ability to inhibit growth of L1210 mouse leukemia cells	[PMID: 2871191]
L1210	IC₅₀	0.002 3 Compound: 2	Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure. Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure.	[PMID: 1992118]
L1210	IC₅₀	0.02 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the enzyme Dihydro Folate Reductase (DHFR) from murine L1210 leukemia cells. Compound was tested for its inhibitory concentration to inhibit the enzyme Dihydro Folate Reductase (DHFR) from murine L1210 leukemia cells.	[PMID: 3462394]
L1210	IC₅₀	0.002 3 Compound: AMT	Compound was tested for its ability to inhibit growth of L1210 mouse leukemia cells Compound was tested for its ability to inhibit growth of L1210 mouse leukemia cells	[PMID: 2871191]
L1210	IC₅₀	84 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells and a subline (L1210 / R81). Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells and a subline (L1210 / R81).	[PMID: 3462394]
L1210	IC₅₀	0.002 3 Compound: AMT	Inhibitory activity against dihydrofolate reductase(DHFR) isolated from L1210 murine leukemia cells Inhibitory activity against dihydrofolate reductase(DHFR) isolated from L1210 murine leukemia cells	[PMID: 3351853]
L1210	IC₅₀	0.002 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells. Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells.	[PMID: 3462394]
L1210	IC₅₀	0.003 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells. Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells.	[PMID: 3462394]
L1210	IC₅₀	0.002 3 Compound: AMT	Inhibitory concentration against growth of L1210 cell line Inhibitory concentration against growth of L1210 cell line	[PMID: 3385730]
L1210	IC₅₀	0.002 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells. Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells.	[PMID: 3462394]
L1210	IC₅₀	0.002 3 Compound: AMT	Inhibitory activity against dihydrofolate reductase(DHFR) isolated from L1210 murine leukemia cells Inhibitory activity against dihydrofolate reductase(DHFR) isolated from L1210 murine leukemia cells	[PMID: 3351853]
L1210	IC₅₀	0.002 3 Compound: AMT	Cell growth inhibition against mouse leukemia L1210 cells Cell growth inhibition against mouse leukemia L1210 cells	[PMID: 3872941]
L1210	IC₅₀	0.002 3 Compound: AMT	Inhibitory concentration against growth of L1210 cell line Inhibitory concentration against growth of L1210 cell line	[PMID: 3385730]
L1210	IC₅₀	0.002 3 Compound: Aminopterin	Compound was evaluated for cell growth inhibition of murine (L1210) leukemic cells Compound was evaluated for cell growth inhibition of murine (L1210) leukemic cells	[PMID: 1992122]
L1210	IC₅₀	0.002 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against mouse leukemic L1210 cells Tested for cell-growth inhibition against mouse leukemic L1210 cells	[PMID: 2898531]
L1210	IC₅₀	0.003 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells. Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells.	[PMID: 3462394]
L1210	IC₅₀	0.025 3 Compound: 10 (AMT)	Tested for inhibition against purified Dihydrofolate reductase from L1210 murine leukemia cells Tested for inhibition against purified Dihydrofolate reductase from L1210 murine leukemia cells	[PMID: 2898531]
L1210 ( R81)	IC₅₀	84 3 Compound: AMT	Compound was tested for its ability to inhibit growth of L1210/R81 cells Compound was tested for its ability to inhibit growth of L1210/R81 cells	[PMID: 2871191]
L1210	IC₅₀	0.02 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the enzyme Dihydro Folate Reductase (DHFR) from murine L1210 leukemia cells. Compound was tested for its inhibitory concentration to inhibit the enzyme Dihydro Folate Reductase (DHFR) from murine L1210 leukemia cells.	[PMID: 3462394]
L1210	IC₅₀	0.025 3 Compound: 10 (AMT)	Tested for inhibition against purified Dihydrofolate reductase from L1210 murine leukemia cells Tested for inhibition against purified Dihydrofolate reductase from L1210 murine leukemia cells	[PMID: 2898531]
L1210 ( R81)	IC₅₀	0.035 3 Compound: AMT	Compound was tested for its ability to inhibit purified dihydrofolate reductase(DHFR) from L1210/R81 cells Compound was tested for its ability to inhibit purified dihydrofolate reductase(DHFR) from L1210/R81 cells	[PMID: 2871191]
L1210	IC₅₀	0.72 1 Compound: AMT	Compound was evaluated for inhibitory effect on growth of L1210 cells at IC50 (n=4) Compound was evaluated for inhibitory effect on growth of L1210 cells at IC50 (n=4)	[PMID: 2423690]
L1210 ( R81)	IC₅₀	84 3 Compound: AMT	Inhibitory concentration against growth of L1210/R81 cell line Inhibitory concentration against growth of L1210/R81 cell line	[PMID: 3385730]
L1210	IC₅₀	1.2 1 Compound: Aminopterin	In vitro inhibition of L1210 cell growth in rodent neoplastic cells In vitro inhibition of L1210 cell growth in rodent neoplastic cells	[PMID: 7108907]
L1210 ( R81)	IC₅₀	84 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against mouse leukemic L1210/R81 cells Tested for cell-growth inhibition against mouse leukemic L1210/R81 cells	[PMID: 2898531]
L1210	IC₅₀	2 1 Compound: aminopterin(AMT)	Compound was evaluated for the growth inhibition of L1210 cells. Compound was evaluated for the growth inhibition of L1210 cells.	[PMID: 1995880]
L929	IC₅₀	0.0023 3 Compound: Aminopterin	Evaluated in vitro for ability to inhibit the L cell lines. Evaluated in vitro for ability to inhibit the L cell lines.	[PMID: 2299624]
L1210	IC₅₀	7.9 3 Compound: AMT	Cell growth inhibition against mouse leukemia L1210/R71 cells Cell growth inhibition against mouse leukemia L1210/R71 cells	[PMID: 3872941]
PC-12	IC₅₀	>5 3 Compound: 1, TKO-10-1	Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis	[PMID: 23916146]
L1210	IC₅₀	84 3 Compound: AMT	Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells and a subline (L1210 / R81). Compound was tested for its inhibitory concentration to inhibit the growth of wild type L1210 cells and a subline (L1210 / R81).	[PMID: 3462394]
L1210	IC₅₀	84 3 Compound: AMT	Cell growth inhibition against mouse leukemia L1210/R81 cells Cell growth inhibition against mouse leukemia L1210/R81 cells	[PMID: 3872941]
L1210	IC₅₀	2.1 x 10^-9 41 Compound: Aminopterin	Inhibitory concentration against the growth of L1210 murine leukemia cells in tissue culture. Inhibitory concentration against the growth of L1210 murine leukemia cells in tissue culture.	[PMID: 4009615]
L1210	IC₅₀	0.6 x 10^-9 41 Compound: Aminopterin	Inhibitory concentration of compound in growth of L-1210 cells Inhibitory concentration of compound in growth of L-1210 cells	[PMID: 6793726]
L1210 ( R81)	IC₅₀	0.035 3 Compound: AMT	Compound was tested for its ability to inhibit purified dihydrofolate reductase(DHFR) from L1210/R81 cells Compound was tested for its ability to inhibit purified dihydrofolate reductase(DHFR) from L1210/R81 cells	[PMID: 2871191]
L1210 ( R81)	IC₅₀	84 3 Compound: 10 (AMT)	Tested for cell-growth inhibition against mouse leukemic L1210/R81 cells Tested for cell-growth inhibition against mouse leukemic L1210/R81 cells	[PMID: 2898531]
L1210 ( R81)	IC₅₀	84 3 Compound: AMT	Compound was tested for its ability to inhibit growth of L1210/R81 cells Compound was tested for its ability to inhibit growth of L1210/R81 cells	[PMID: 2871191]
L1210 ( R81)	IC₅₀	84 3 Compound: AMT	Inhibitory concentration against growth of L1210/R81 cell line Inhibitory concentration against growth of L1210/R81 cell line	[PMID: 3385730]
L929	IC₅₀	0.0023 3 Compound: Aminopterin	Evaluated in vitro for ability to inhibit the L cell lines. Evaluated in vitro for ability to inhibit the L cell lines.	[PMID: 2299624]
PC-12	IC₅₀	> 5 3 Compound: 1, TKO-10-1	Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis	[PMID: 23916146]
SCC-25	IC₅₀	6.9 1 Compound: AMT	Tested for inhibitory concentration against human SCC25 cell line Tested for inhibitory concentration against human SCC25 cell line	[PMID: 8035423]
SCC-7	IC₅₀	4 1 Compound: AMT	Tested for inhibitory concentration against SCC-VII murine squamous carcinoma cell line Tested for inhibitory concentration against SCC-VII murine squamous carcinoma cell line	[PMID: 8035423]

In Vitro

The IC₅₀ value of Aminopterin (4-Aminofolic acid) against CCRF-CEM cells during 72 h of exposure is 4.4 nM^[2].
Aminopterin (4-Aminofolic acid) produces a marked inhibition of mitosis in low concentrations in human leukemic leukocytes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

440.41

Formula

C₁₉H₂₀N₈O₅

CAS No.

54-62-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

NC1=NC(N)=C2C(N=CC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)=N2)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

1 M NaOH : 100 mg/mL (227.06 mM; ultrasonic and warming and heat to 60°C)

DMSO : 31.25 mg/mL (70.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2706 mL	11.3531 mL	22.7061 mL
5 mM	0.4541 mL	2.2706 mL	4.5412 mL
10 mM	0.2271 mL	1.1353 mL	2.2706 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.67%

Select Batch:

Data Sheet (271 KB) SDS (644 KB)

COA (247 KB) HNMR (197 KB) RP-HPLC (277 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. MEYER LM, et al. Aminopterin, a folic acid antagonist, in the treatment of leukemia. Am J Clin Pathol. 1949 Feb;19(2):119-26. [Content Brief]

[2]. Rosowsky A, et al. Synthesis and in vitro antifolate activity of rotationally restricted aminopterin and methotrexate analogues. J Med Chem. 2004 Dec 30;47(27):6958-63. [Content Brief]

[3]. Gebhardt DO, et al. The influence of aminopterin on limb regeneration in Ambystoma mexicanum. J Embryol Exp Morphol. 1966 Aug;16(1):143-58. [Content Brief]

[4]. F. W Gtmrz, et al. THE EFFECT OF 4-AMINO-PTEROYLGLUTAMIC ACID (AMINOPTERIN) ON HUMAN LEUKEMIC LEUKOCYTES IN VITRO. Blood (1950) 5 (2): 161-166.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / 1 M NaOH	1 mM	2.2706 mL	11.3531 mL	22.7061 mL	56.7653 mL
	5 mM	0.4541 mL	2.2706 mL	4.5412 mL	11.3531 mL
	10 mM	0.2271 mL	1.1353 mL	2.2706 mL	5.6765 mL
	15 mM	0.1514 mL	0.7569 mL	1.5137 mL	3.7844 mL
	20 mM	0.1135 mL	0.5677 mL	1.1353 mL	2.8383 mL
	25 mM	0.0908 mL	0.4541 mL	0.9082 mL	2.2706 mL
	30 mM	0.0757 mL	0.3784 mL	0.7569 mL	1.8922 mL
	40 mM	0.0568 mL	0.2838 mL	0.5677 mL	1.4191 mL
	50 mM	0.0454 mL	0.2271 mL	0.4541 mL	1.1353 mL
	60 mM	0.0378 mL	0.1892 mL	0.3784 mL	0.9461 mL
1 M NaOH	80 mM	0.0284 mL	0.1419 mL	0.2838 mL	0.7096 mL
1 M NaOH	100 mM	0.0227 mL	0.1135 mL	0.2271 mL	0.5677 mL

Aminopterin Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Aminopterin54-62-64-Aminofolic acid APGAAntifolateleukemicleukocytesfolicacidtetrahydrofolicanticancerimmunosuppressiveInhibitorinhibitorinhibit

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Aminopterin (Synonyms: 4-Aminofolic acid; APGA)

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