1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Tetrahydrofolic acid

Tetrahydrofolic acid (Synonyms: L-5,6,7,8-Tetrahydrofolic acid; L-Tetrahydrofolic acid)

Cat. No.: HY-14520
Handling Instructions

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

For research use only. We do not sell to patients.

Tetrahydrofolic acid Chemical Structure

Tetrahydrofolic acid Chemical Structure

CAS No. : 135-16-0

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Based on 1 publication(s) in Google Scholar

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Description

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid[1].

IC50 & Target[1]

Human Endogenous Metabolite

 

In Vitro

Tetrahydrofolic acid (0-200 µM; 3 days; Adh5-/- DT40 cells) exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks[1].
Tetrahydrofolic acid (0-100 µM; 16 hours; Adh5-/- DT40 cells) treatment strongly promots FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner[1].
Tetrahydrofolic acid exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 3 days
Result: Viability of Adh5-/- DT40 cells rapidly dropped.

Western Blot Analysis[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 16 hours
Result: Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner.
In Vivo

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5-/- mice) treatment perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid could be mutagenic and genotoxic to bone marrow cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adh5-/- mice[1]
Dosage: 62.5 mg/kg
Administration: Intraperitoneal injection; daily
Result: Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).
Molecular Weight

445.43

Formula

C₁₉H₂₃N₇O₆

CAS No.

135-16-0

SMILES

NC(NC1=C2NC(CNC3=CC=C(C(N[[email protected]@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

Tetrahydrofolic acidL-5,6,7,8-Tetrahydrofolic acid L-Tetrahydrofolic acidEndogenous MetaboliteFolatederivativewater-solublevitamin5,10-methylenetetrahydrofolatehematopoiesisFANCD2one-carbonDNAaminonucleiclipidsInhibitorinhibitorinhibit

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Tetrahydrofolic acid
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HY-14520
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