1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Tetrahydrofolic acid

Tetrahydrofolic acid (Synonyms: L-5,6,7,8-Tetrahydrofolic acid; L-Tetrahydrofolic acid)

Cat. No.: HY-14520 Purity: 90.34%
Handling Instructions

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

For research use only. We do not sell to patients.

Tetrahydrofolic acid Chemical Structure

Tetrahydrofolic acid Chemical Structure

CAS No. : 135-16-0

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25 mg USD 50 In-stock
Estimated Time of Arrival: December 31
50 mg USD 80 In-stock
Estimated Time of Arrival: December 31
100 mg USD 130 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid[1].

IC50 & Target[1]

Human Endogenous Metabolite

 

In Vitro

Tetrahydrofolic acid (0-200 µM; 3 days; Adh5-/- DT40 cells) exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks[1].
Tetrahydrofolic acid (0-100 µM; 16 hours; Adh5-/- DT40 cells) treatment strongly promots FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner[1].
Tetrahydrofolic acid exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 3 days
Result: Viability of Adh5-/- DT40 cells rapidly dropped.

Western Blot Analysis[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 16 hours
Result: Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner.
In Vivo

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5-/- mice) treatment perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid could be mutagenic and genotoxic to bone marrow cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adh5-/- mice[1]
Dosage: 62.5 mg/kg
Administration: Intraperitoneal injection; daily
Result: Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).
Molecular Weight

445.43

Formula

C₁₉H₂₃N₇O₆

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (46.76 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2450 mL 11.2251 mL 22.4502 mL
5 mM 0.4490 mL 2.2450 mL 4.4900 mL
10 mM 0.2245 mL 1.1225 mL 2.2450 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Product Name:
Tetrahydrofolic acid
Cat. No.:
HY-14520
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