2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Tetrahydrofolic acid

Tetrahydrofolic acid  (Synonyms: L-5,6,7,8-Tetrahydrofolic acid; L-Tetrahydrofolic acid)

Cat. No.: HY-14520 Purity: 96.35%
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Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

For research use only. We do not sell to patients.

Tetrahydrofolic acid Chemical Structure

Tetrahydrofolic acid Chemical Structure

CAS No. : 135-16-0

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Based on 1 publication(s) in Google Scholar

Other Forms of Tetrahydrofolic acid:

Tetrahydrofolic acid Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Tetrahydrofolic acid

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid[1].

IC50 & Target[1]

Human Endogenous Metabolite

 

In Vitro

Tetrahydrofolic acid (0-200 μM; 3 days; Adh5-/- DT40 cells) exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks[1].
Tetrahydrofolic acid (0-100 μM; 16 hours; Adh5-/- DT40 cells) treatment strongly promots FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner[1].
Tetrahydrofolic acid exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tetrahydrofolic acid Related Antibodies

Cell Viability Assay[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 3 days
Result: Viability of Adh5-/- DT40 cells rapidly dropped.

Western Blot Analysis[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 16 hours
Result: Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner.
In Vivo

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5-/- mice) treatment perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid could be mutagenic and genotoxic to bone marrow cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adh5-/- mice[1]
Dosage: 62.5 mg/kg
Administration: Intraperitoneal injection; daily
Result: Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).
Molecular Weight

445.43

Formula

C19H23N7O6
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

NC(NC1=C2NC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O
Structure Classification
Initial Source
Shipping

Shipping with dry ice.
Storage

-80°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (46.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2450 mL 11.2251 mL 22.4502 mL
5 mM 0.4490 mL 2.2450 mL 4.4900 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 96.35%

COA (247 KB) RP-HPLC (253 KB) MS (182 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2450 mL 11.2251 mL 22.4502 mL 56.1255 mL
5 mM 0.4490 mL 2.2450 mL 4.4900 mL 11.2251 mL
10 mM 0.2245 mL 1.1225 mL 2.2450 mL 5.6126 mL
15 mM 0.1497 mL 0.7483 mL 1.4967 mL 3.7417 mL
20 mM 0.1123 mL 0.5613 mL 1.1225 mL 2.8063 mL
25 mM 0.0898 mL 0.4490 mL 0.8980 mL 2.2450 mL
30 mM 0.0748 mL 0.3742 mL 0.7483 mL 1.8709 mL
40 mM 0.0561 mL 0.2806 mL 0.5613 mL 1.4031 mL
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Tetrahydrofolic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetrahydrofolic acid135-16-0L-5,6,7,8-Tetrahydrofolic acid L-Tetrahydrofolic acidEndogenous MetaboliteFolatederivativewater-solublevitamin5,10-methylenetetrahydrofolatehematopoiesisFANCD2one-carbonDNAaminonucleiclipidsInhibitorinhibitorinhibit

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