Cat. No.: HY-14520

Tetrahydrofolic acid

Name: Tetrahydrofolic acid
Brand: MedChemExpress
SKU: HY-14520
Price: 32.00 USD
Availability: InStock
Rating: 5 (6 reviews)

(Synonyms: L-5,6,7,8-Tetrahydrofolic acid; L-Tetrahydrofolic acid)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

For research use only. We do not sell to patients.

Purity : 96.77%
CAS No.: 135-16-0
Formula: C₁₉H₂₃N₇O₆
Molecular Weight:445.43

Storage:

-80°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Tetrahydrofolic acid Chemical Structure

CAS No.: 135-16-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Tetrahydrofolic acid (0-200 μM; 3 days; Adh5^-/- DT40 cells) exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks^[1].
Tetrahydrofolic acid (0-100 μM; 16 hours; Adh5^-/- DT40 cells) treatment strongly promots FANCD2 and ser139-H2AX focus formation in Adh5^-/- cells in a dose-dependent manner^[1].
Tetrahydrofolic acid exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Adh5^-/- DT40 cells
Concentration:

0-200 µM
Incubation Time:

3 days
Result:

Viability of Adh5^-/- DT40 cells rapidly dropped.

Western Blot Analysis^[1]

Cell Line:

Adh5^-/- DT40 cells
Concentration:

0-200 µM
Incubation Time:

16 hours
Result:

Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5^-/- cells in a dose-dependent manner.

In Vivo

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5^-/- mice) treatment perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid could be mutagenic and genotoxic to bone marrow cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adh5^-/- mice^[1]
Dosage:

62.5 mg/kg
Administration:

Intraperitoneal injection; daily
Result:

Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).

Chemical Information

CAS No. 135-16-0
Appearance Solid
Molecular Weight 445.43
Formula C₁₉H₂₃N₇O₆
Color Light brown to brown
SMILES

NC(NC1=C2NC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O
Synonyms

L-5,6,7,8-Tetrahydrofolic acid; L-Tetrahydrofolic acid
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Shipping with dry ice.
Storage

-80°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (6)

All 2D/3D Cell Culture and DifferentiationBio/Physico-chemical AssayCell Proliferation/Viability AssayCell Imaging/Staining

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Journal Impact Factor

Nat Commun

Methylcobalamin protects against liver failure via engaging gasdermin E. [Abstract]

2025 Jan 31;16(1):1233. PMID: 39890804

Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]

Micehepatocytes were pretreated with indicated concentrations of L-methionine (L-Met) or tetrahydrofolic acid (THF) for 2 h before adding 200 μM DCA for 4 h.
J Adv Res

A novel zebrafish model unveils folate deficiency disrupts embryonic development via p53-driven cell cycle arrest. [Abstract]

2026 Mar 5:S2090-1232(26)00188-8. PMID: 41794221
Mol Cell

Microprotein SMIM26 drives oxidative metabolism via serine-responsive mitochondrial translation. [Abstract]

2025 Jul 17;85(14):2759-2775.e12. PMID: 40578345
Int J Biol Macromol

Tetrahydrofolic acid accelerates amyloid fibrillization, decreases cytotoxic oligomers and suppresses their toxicity. [Abstract]

2025 Feb:290:139041. PMID: 39708879

Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

The effect of Tetrahydrofolic acid (25-75 μM) on the aggregation of αS and Aβ was monitored by ThT fluorescence.

Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

αS, Aβ40, and Aβ42 were incubated for 340 h, 110 h, and 20 h, respectively, in the absence or presence of a specified proportion of Tetrahydrofolic acid for AFM analysis. Scale bars = 1 μm.

Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

The effect of 50 μM αS, 25 μM Aβ40 and 25 μM Aβ42 aggregates on cell viability without or with different concentrations of Tetrahydrofolic acid (25-100 μM; 48 h) was determined by MTT assay.

Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

Tetrahydrofolic acid (75-100 μM; 72 h) accelerates amyloid aggregation in C. elegans.
J Biol Chem

Elucidation of the substrate of tRNA-modifying enzymes MnmEG leads to in vitro reconstitution of an evolutionarily conserved uridine hypermodification. [Abstract]

2022 Nov;298(11):102548. PMID: 36181794
J Pharm Biomed Anal

A rapid LC-MS/MS method for the simultaneous quantification of 10 key folate cycle intermediates in human plasma. [Abstract]

2026 Jan 15:268:117178. PMID: 41061512

Solvent & Solubility

In Vitro:

DMSO : 116.67 mg/mL (261.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2450 mL

11.2251 mL

22.4502 mL

5 mM

0.4490 mL

2.2450 mL

4.4900 mL

10 mM

0.2245 mL

1.1225 mL

2.2450 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Clara B García-Calderón, et al. Genotoxicity of Tetrahydrofolic Acid to Hematopoietic Stem and Progenitor Cells. Cell Death Differ. 2018 Nov;25(11):1967-1979. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2450 mL	11.2251 mL	22.4502 mL	56.1255 mL
	5 mM	0.4490 mL	2.2450 mL	4.4900 mL	11.2251 mL
	10 mM	0.2245 mL	1.1225 mL	2.2450 mL	5.6126 mL
	15 mM	0.1497 mL	0.7483 mL	1.4967 mL	3.7417 mL
	20 mM	0.1123 mL	0.5613 mL	1.1225 mL	2.8063 mL
	25 mM	0.0898 mL	0.4490 mL	0.8980 mL	2.2450 mL
	30 mM	0.0748 mL	0.3742 mL	0.7483 mL	1.8709 mL
	40 mM	0.0561 mL	0.2806 mL	0.5613 mL	1.4031 mL
	50 mM	0.0449 mL	0.2245 mL	0.4490 mL	1.1225 mL
	60 mM	0.0374 mL	0.1871 mL	0.3742 mL	0.9354 mL
	80 mM	0.0281 mL	0.1403 mL	0.2806 mL	0.7016 mL
	100 mM	0.0225 mL	0.1123 mL	0.2245 mL	0.5613 mL

Bioz

Tetrahydrofolic acid

6 Cited Publications

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Tetrahydrofolic acid

Publications Citing Use of MedChemExpress (MCE) Tetrahydrofolic acid

All Endogenous Metabolite Isoforms