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Cat. No.: HY-14520B

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Tetrahydrofolic acid trihydrochloride

Name: Tetrahydrofolic acid trihydrochloride
Brand: MedChemExpress
SKU: HY-14520B
Availability: PreOrder

(Synonyms: L-5,6,7,8-Tetrahydrofolic acid trihydrochloride; L-Tetrahydrofolic acid trihydrochloride)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) trihydrochloride is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid trihydrochloride is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid trihydrochloride serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

For research use only. We do not sell to patients.

CAS No.: 150731-85-4
Formula: C₁₉H₂₆Cl₃N₇O₆
Molecular Weight:554.81

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

Tetrahydrofolic acid trihydrochloride Chemical Structure

CAS No.: 150731-85-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Tetrahydrofolic acid (0-200 μM; 3 days; Adh5^-/- DT40 cells) trihydrochloride exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks^[1].
Tetrahydrofolic acid (0-100 μM; 16 hours; Adh5^-/- DT40 cells) trihydrochloride strongly promots FANCD2 and ser139-H2AX focus formation in Adh5^-/- cells in a dose-dependent manner^[1].
Tetrahydrofolic acid trihydrochloride exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Adh5^-/- DT40 cells
Concentration:

0-200 µM
Incubation Time:

3 days
Result:

Viability of Adh5^-/-DT40 cells rapidly dropped.

Western Blot Analysis^[1]

Cell Line:

Adh5^-/- DT40 cells
Concentration:

0-200 µM
Incubation Time:

16 hours
Result:

Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5^-/- cells in a dose-dependent manner.

In Vivo

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5^-/- mice) trihydrochloride perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid trihydrochloride could be mutagenic and genotoxic to bone marrow cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adh5^-/- mice^[1]
Dosage:

62.5 mg/kg
Administration:

Intraperitoneal injection; daily
Result:

Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).

Chemical Information

CAS No. 150731-85-4
Molecular Weight 554.81
Formula C₁₉H₂₆Cl₃N₇O₆
SMILES

NC(NC1=C2NC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O.Cl.Cl.Cl
Synonyms

L-5,6,7,8-Tetrahydrofolic acid trihydrochloride; L-Tetrahydrofolic acid trihydrochloride
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (6)

All 2D/3D Cell Culture and DifferentiationBio/Physico-chemical AssayCell Proliferation/Viability AssayCell Imaging/Staining

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Journal Impact Factor

Nat Commun

Methylcobalamin protects against liver failure via engaging gasdermin E. [Abstract]

2025 Jan 31;16(1):1233. PMID: 39890804

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]

Micehepatocytes were pretreated with indicated concentrations of L-methionine (L-Met) or tetrahydrofolic acid (THF) for 2 h before adding 200 μM DCA for 4 h.
J Adv Res

A novel zebrafish model unveils folate deficiency disrupts embryonic development via p53-driven cell cycle arrest. [Abstract]

2026 Mar 5:S2090-1232(26)00188-8. PMID: 41794221
Mol Cell

Microprotein SMIM26 drives oxidative metabolism via serine-responsive mitochondrial translation. [Abstract]

2025 Jul 17;85(14):2759-2775.e12. PMID: 40578345
Int J Biol Macromol

Tetrahydrofolic acid accelerates amyloid fibrillization, decreases cytotoxic oligomers and suppresses their toxicity. [Abstract]

2025 Feb:290:139041. PMID: 39708879

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

The effect of Tetrahydrofolic acid (25-75 μM) on the aggregation of αS and Aβ was monitored by ThT fluorescence.

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

αS, Aβ40, and Aβ42 were incubated for 340 h, 110 h, and 20 h, respectively, in the absence or presence of a specified proportion of Tetrahydrofolic acid for AFM analysis. Scale bars = 1 μm.

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

The effect of 50 μM αS, 25 μM Aβ40 and 25 μM Aβ42 aggregates on cell viability without or with different concentrations of Tetrahydrofolic acid (25-100 μM; 48 h) was determined by MTT assay.

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

Tetrahydrofolic acid (75-100 μM; 72 h) accelerates amyloid aggregation in C. elegans.
J Biol Chem

Elucidation of the substrate of tRNA-modifying enzymes MnmEG leads to in vitro reconstitution of an evolutionarily conserved uridine hypermodification. [Abstract]

2022 Nov;298(11):102548. PMID: 36181794
J Pharm Biomed Anal

A rapid LC-MS/MS method for the simultaneous quantification of 10 key folate cycle intermediates in human plasma. [Abstract]

2026 Jan 15:268:117178. PMID: 41061512

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Steven W. Bailey, et al. Synthesis of Tetrahydropteridine C6-Stereoisomers, Including N6-Formyl-(68)-tetrahydrofolic Acid. The Journal of Organic Chemistry, 57(16), 4470-4477. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Tetrahydrofolic acid trihydrochloride

6 Cited Publications

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Purity & Documentation
References

1/16
1/16

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]

Customer Validation
Micehepatocytes were pretreated with indicated concentrations of L-methionine (L-Met) or tetrahydrofolic acid (THF) for 2 h before adding 200 μM DCA for 4 h.
1/16

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

Customer Validation
The effect of Tetrahydrofolic acid (25-75 μM) on the aggregation of αS and Aβ was monitored by ThT fluorescence.
1/16

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

Customer Validation
αS, Aβ40, and Aβ42 were incubated for 340 h, 110 h, and 20 h, respectively, in the absence or presence of a specified proportion of Tetrahydrofolic acid for AFM analysis. Scale bars = 1 μm.
1/16

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

Customer Validation
The effect of 50 μM αS, 25 μM Aβ40 and 25 μM Aβ42 aggregates on cell viability without or with different concentrations of Tetrahydrofolic acid (25-100 μM; 48 h) was determined by MTT assay.
1/16

Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Feb:290:139041. [Abstract]

Customer Validation
Tetrahydrofolic acid (75-100 μM; 72 h) accelerates amyloid aggregation in C. elegans.

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