2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Tetrahydrofolic acid trihydrochloride

Tetrahydrofolic acid trihydrochloride  (Synonyms: L-5,6,7,8-Tetrahydrofolic acid trihydrochloride; L-Tetrahydrofolic acid trihydrochloride)

Cat. No.: HY-14520B
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Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) trihydrochloride is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid trihydrochloride is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid trihydrochloride serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

For research use only. We do not sell to patients.

CAS No. : 150731-85-4

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Tetrahydrofolic acid trihydrochloride Related Antibodies

Top Publications Citing Use of Products

    Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233.  [Abstract]

    Micehepatocytes were pretreated with indicated concentrations of L-methionine (L-Met) or tetrahydrofolic acid (THF) for 2 h before adding 200 μM DCA for 4 h.

    Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    The effect of Tetrahydrofolic acid (25-75 μM) on the aggregation of αS and Aβ was monitored by ThT fluorescence.

    Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    αS, Aβ40, and Aβ42 were incubated for 340 h, 110 h, and 20 h, respectively, in the absence or presence of a specified proportion of Tetrahydrofolic acid for AFM analysis. Scale bars = 1 μm.

    Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    The effect of 50 μM αS, 25 μM Aβ40 and 25 μM Aβ42 aggregates on cell viability without or with different concentrations of Tetrahydrofolic acid (25-100 μM; 48 h) was determined by MTT assay.

    Tetrahydrofolic acid trihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    Tetrahydrofolic acid (75-100 μM; 72 h) accelerates amyloid aggregation in C. elegans.

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    Description

    Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) trihydrochloride is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid trihydrochloride is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid trihydrochloride serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Tetrahydrofolic acid (0-200 μM; 3 days; Adh5-/- DT40 cells) trihydrochloride exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks[1].
    Tetrahydrofolic acid (0-100 μM; 16 hours; Adh5-/- DT40 cells) trihydrochloride strongly promots FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner[1].
    Tetrahydrofolic acid trihydrochloride exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tetrahydrofolic acid trihydrochloride Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Adh5-/- DT40 cells
    Concentration: 0-200 µM
    Incubation Time: 3 days
    Result: Viability of Adh5-/-DT40 cells rapidly dropped.

    Western Blot Analysis[1]

    Cell Line: Adh5-/- DT40 cells
    Concentration: 0-200 µM
    Incubation Time: 16 hours
    Result: Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner.
    In Vivo

    Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5-/- mice) trihydrochloride perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid trihydrochloride could be mutagenic and genotoxic to bone marrow cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adh5-/- mice[1]
    Dosage: 62.5 mg/kg
    Administration: Intraperitoneal injection; daily
    Result: Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).
    Molecular Weight

    554.81

    Formula

    C19H26Cl3N7O6
    CAS No.
    SMILES

    NC(NC1=C2NC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O.Cl.Cl.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Tetrahydrofolic acid trihydrochloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tetrahydrofolic acid trihydrochloride150731-85-4L-5,6,7,8-Tetrahydrofolic acid trihydrochloride L-Tetrahydrofolic acid trihydrochlorideEndogenous MetaboliteFolatederivativewater-solublevitamin5,10-methylenetetrahydrofolatehematopoiesisFANCD2one-carbonDNAaminonucleiclipidsInhibitorinhibitorinhibit

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