Tetrahydrofolic acid trihydrochloride  (Synonyms: L-5,6,7,8-Tetrahydrofolic acid trihydrochloride; L-Tetrahydrofolic acid trihydrochloride)

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) trihydrochloride is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid trihydrochloride is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid trihydrochloride serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid^[1].

Tetrahydrofolic acid (0-200 μM; 3 days; Adh5^-/- DT40 cells) trihydrochloride exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks^[1].
Tetrahydrofolic acid (0-100 μM; 16 hours; Adh5^-/- DT40 cells) trihydrochloride strongly promots FANCD2 and ser139-H2AX focus formation in Adh5^-/- cells in a dose-dependent manner^[1].
Tetrahydrofolic acid trihydrochloride exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5^-/- mice) trihydrochloride perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid trihydrochloride could be mutagenic and genotoxic to bone marrow cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

554.81

C₁₉H₂₆Cl₃N₇O₆

150731-85-4

NC(NC1=C2NC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O.Cl.Cl.Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Steven W. Bailey, et al. Synthesis of Tetrahydropteridine C6-Stereoisomers, Including N6-Formyl-(68)-tetrahydrofolic Acid. The Journal of Organic Chemistry, 57(16), 4470-4477.  [Content Brief]