Indoximod (Synonyms: 1-Methyl-D-tryptophan; NLG-8189)

Name: Indoximod
Brand: MedChemExpress
SKU: HY-16724
Rating: 5.0 (11 reviews)

Cat. No.: HY-16724 Purity: 99.54%: Data Sheet
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Indoximod (1-Methyl-D-tryptophan) is an orally active indoleamine 2,3-dioxygenase (IDO) pathway inhibitor. Indoximod acts as a Trp mimetic in regulating mTOR. Indoximod is an immunometabolic adjuvant used for the research of cancer.

For research use only. We do not sell to patients.

CAS No. : 110117-83-4

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100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 11 publication(s) in Google Scholar

Other Forms of Indoximod:

(Rac)-Indoximod In-stock
(S)-Indoximod In-stock
Indoximod (Standard) Get quote

11 Publications Citing Use of MCE Indoximod

Bio/Physico-chemical Assay

In Vivo Efficacy Study

Flow Cytometry

Histological Imaging/Staining

RT-PCR

: Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269. [Abstract]; Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) resulted in significant weight gain in the BLM-induced mouse model.

: Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269. [Abstract]; Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) markedly improved overall survival compared with the BLM-treated group.

: Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269. [Abstract]; Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) reversed visual pulmonary injury and collagen deposition, attenuated the increase in lung coefficient, and prevented the reduction in terminal body weight in the BLM-induced mouse model.

: Indoximod purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Sep:237:251-269. [Abstract]; Indoximod (20 mg/kg, oral gavage, 14 days) or Varespladib (30 mg/kg, oral gavage, 14 days) reversed the BLM-induced upregulation of Pla2g2a, Ido1, Acta2, and Col1a1 gene expression.

: Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108. [Abstract]; Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) increased renal injury in IgAN mice.

: Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108. [Abstract]; Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) promoted mesangial proliferation and collagen fibrillogenesis.

: Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108. [Abstract]; Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) promoted glomerular IgA accumulation.

: Indoximod purchased from MedChemExpress. Usage Cited in: Folia Biol (Praha). 2019;65(2):101-108. [Abstract]; Indoximod (1-MT，injected intraperitoneally, 1 mg/day, from the 8th week to the 11th week) increased the proportion of Th17 cells and decreased the proportion of Treg cells in the kidney tissue of IgAN mice.

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IDO1 IDO2 IDO

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

IDO

19 μM (Ki)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: D-MT	Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 31954880]
HUVEC	IC₅₀	> 100 μM Compound: D-MT	Cytotoxicity against HUVEC cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Cytotoxicity against HUVEC cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 31954880]
HeLa	EC₅₀	110 μM Compound: L-1MT	Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells using L-tryptophan as substrate after 24 hrs Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells using L-tryptophan as substrate after 24 hrs	[PMID: 28526475]
HeLa	IC₅₀	> 100 μM Compound: D-MT	Anticancer activity against human HeLa cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human HeLa cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 31954880]
PC-3	IC₅₀	> 100 μM Compound: D-MT	Anticancer activity against human PC3 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human PC3 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 31954880]
U-87MG ATCC	IC₅₀	148.9 μM Compound: 1-D-MT	Inhibition of recombinant human IDO2 expressed in human U87MG cells assessed as reduction in kynurenine formation using L-tryptophan as substrate after 6 hrs by spectrophotometry Inhibition of recombinant human IDO2 expressed in human U87MG cells assessed as reduction in kynurenine formation using L-tryptophan as substrate after 6 hrs by spectrophotometry	[PMID: 27475108]

In Vitro

The IDO inhibitor 1-methyl-tryptophan exists in two stereoisomers with potentially different biological properties. The L isomer is the more potent inhibitor of IDO activity using the purified enzyme and in HeLa cell–based assays. However, the D isomer is significantly more effective in reversing the suppression of T cells created by IDO-expressing dendritic cells. The L isomer of 1-methyl-tryptophan functioned as a competitive inhibitor (K_i=19 μM), whereas the d isomer is much less effective. The DL mixture is intermediate, with a K_i of 35 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The D isomer is more efficacious as an anticancer agent in chemo-immunotherapy regimens using NSC-26271, NSC 125973, or LY 188011, when tested in mouse models of transplantable melanoma and transplantable and autochthonous breast cancer. The D isomer of 1-methyl-tryptophan specifically targets the IDO gene because the antitumor effect of d-1-methyl-tryptophan is completely lost in mice with a disruption of the IDO gene (IDO-knockout mice). Oral administration of dl-1-methyl-tryptophan in combination with NSC 125973 can elicit regression of autochthonous breast tumors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

218.26

Formula

C₁₂H₁₄N₂O₂

CAS No.

110117-83-4

Appearance

Solid

Color

White to off-white

SMILES

N[C@H](CC1=CN(C)C2=CC=CC=C12)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

0.5 M NaOH : 66.67 mg/mL (305.46 mM; Need ultrasonic)

H₂O : 5 mg/mL (22.91 mM; ultrasonic and adjust pH to 2 with HCl)

DMSO : 0.55 mg/mL (2.52 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5817 mL	22.9085 mL	45.8169 mL
5 mM	0.9163 mL	4.5817 mL	9.1634 mL
10 mM	0.4582 mL	2.2908 mL	4.5817 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 1 mg/mL (4.58 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (279 KB) SDS (396 KB)

COA (252 KB) HNMR (157 KB) RP-HPLC (258 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Hou DY, et al. Inhibition of indoleamine 2,3-dioxygenase in dendritic cells by stereoisomers of 1-methyl-tryptophancorrelates with antitumor responses. Cancer Res. 2007 Jan 15;67(2):792-801. [Content Brief]

[2]. Metz R, et al. IDO inhibits a tryptophan sufficiency signal that stimulates mTOR: A novel IDO effector pathway targeted by D-1-methyl-tryptophan. Oncoimmunology. 2012;1(9):1460-1468. [Content Brief]

Kinase Assay
^[1]

1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 µM to wells containing the reaction mixture with tryptophan (0-200 µM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 µl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 µl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Hou DY, et al. Inhibition of indoleamine 2,3-dioxygenase in dendritic cells by stereoisomers of 1-methyl-tryptophancorrelates with antitumor responses. Cancer Res. 2007 Jan 15;67(2):792-801. [Content Brief]

[2]. Metz R, et al. IDO inhibits a tryptophan sufficiency signal that stimulates mTOR: A novel IDO effector pathway targeted by D-1-methyl-tryptophan. Oncoimmunology. 2012;1(9):1460-1468. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O / 0.5 M NaOH	1 mM	4.5817 mL	22.9085 mL	45.8169 mL	114.5423 mL
H₂O / 0.5 M NaOH	5 mM	0.9163 mL	4.5817 mL	9.1634 mL	22.9085 mL
	10 mM	0.4582 mL	2.2908 mL	4.5817 mL	11.4542 mL
	15 mM	0.3054 mL	1.5272 mL	3.0545 mL	7.6362 mL
	20 mM	0.2291 mL	1.1454 mL	2.2908 mL	5.7271 mL
0.5 M NaOH	25 mM	0.1833 mL	0.9163 mL	1.8327 mL	4.5817 mL
	30 mM	0.1527 mL	0.7636 mL	1.5272 mL	3.8181 mL
	40 mM	0.1145 mL	0.5727 mL	1.1454 mL	2.8636 mL
	50 mM	0.0916 mL	0.4582 mL	0.9163 mL	2.2908 mL
	60 mM	0.0764 mL	0.3818 mL	0.7636 mL	1.9090 mL
	80 mM	0.0573 mL	0.2864 mL	0.5727 mL	1.4318 mL
	100 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1454 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Indoximod Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indoximod110117-83-41-Methyl-D-tryptophan NLG-8189NLG8189NLG 8189NLG-8189Indoleamine 2,3-Dioxygenase (IDO)tryptophanTrp mimeticimmunotherapeutic vaccinesmTORC1cancerInhibitorinhibitorinhibit

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Indoximod (Synonyms: 1-Methyl-D-tryptophan; NLG-8189)

Customer Review

Other Forms of Indoximod:

Indoximod Related Antibodies

11 Publications Citing Use of MCE Indoximod

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Indoleamine 2,3-Dioxygenase (IDO) Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Indoximod Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Indoximod Related Classifications

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